专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-8871926-B1 优先权日:2010-09-28 标题:Synthesis of porphyrin nanostructures 发明人:FAN HONGYOU; BAI FENG 权利人:FAN HONGYOU; BAI FENG; SANDIA CORP 摘要:The present disclosure generally relates to self-assembly methods for generating porphyrin nanostructures. For example, in one embodiment a method is provided that includes preparing a porphyrin solution and a surfactant solution. The porphyrin solution is then mixed with the surfactant solution at a concentration sufficient for confinement of the porphyrin molecules by the surfactant molecules. In some embodiments, the concentration of the surfactant is at or above its critical micelle concentration (CMC), which allows the surfactant to template the growth of the nanostructure over time. The size and morphology of the nanostructures may be affected by the type of porphyrin molecules used, the type of surfactant used, the concentration of the porphyrin and surfactant the pH of the mixture of the solutions, and the order of adding the reagents to the mixture, to name a few variables.
专利号:US-4303627-A 优先权日:1976-11-11 标题:Crystalline zeolite powder of type A (VI) 发明人:STRACK HANS; ROEBKE WOLFGANG; KNEITEL DIETER; PARR EHRFRIED 权利人:DEGUSSA; HENKEL KGAA 摘要:There are produced crystalline zeolite powders of Type A with the composition 1.0+/-0.2 M2/nO:Al2O3:1.85+/-0.5 SiO2xy H2O where M is a metal cation, n is its valence and y has a value up to 6 with 50 weight % of the particles not over 5.9 mu and with a particle spectrum -Fraction ( mu ) Portion (weight %) -<3 15 to 25 -<5 35 to 55 -<10 80 to 94 -<15 96 to 100 - by hydrothermally crystallizing a SiO2, Al2O3, Na2O and water containing alkali aluminate, water, silicate synthesis mixture with an optional tempering step wherein in a given case during the crystallization or tempering step instead of stirring shearing forces are employed. The process comprises providing water in a container, simultaneously feeding into the water at a temperature of 30 DEG to 100 DEG C. an aqueous sodium aluminate liquor containing 100 to 200 grams/l of Na2O and 50 to 150 grams/l of Al2O3 and alkali silicate solution, diluting with water to obtain a gel containing synthesis premixture having the composition SiO2/Al2O3=2 to 50:1, Na2O/SiO2=0.2 to 20:1 and H2O/Na2O=4 to 300:1 adding further sodium aluminate liquor of the same composition and water having a temperature of 10 DEG to 100 DEG C. with stirring and allowing the thus obtained synthesis mixture to crystallize at a temperature within between 20 DEG and 175 DEG C. in at least 15 minutes.
专利号:US-4305916-A 优先权日:1976-11-11 标 题:Crystalline zeolite powder of type A (III) 发明人:STRACK HANS; ROEBKE WOLFGANG; KNEITEL DIETER; PARR EHRFRIED 权利人:DEGUSSA; HENKEL KGAA 摘要:There are produced crystalline zeolite powders of Type A with the composition 1.0+/-0.2M2/nO:Al2O3:1.85+/-0.5SiO2.yH2O where M is a metal cation, n is it valence and y has a value up to 6 with 50 weight % of the particles not over 4.3 mu and with a particle spectrum: -Fraction ( mu ) Portion (weight %) -<3 18 to 38 -<5 70 to 82 -<10 93 to 99 -<15 96 to 100 - by hydrothermally crystallizing a SiO2, Al2O3, Na2O and water containing alkali aluminate, water, silicate synthesis mixture with an optional tempering step wherein in a given case during the crystallization or tempering step instead of stirring shearing forces are employed. The process comprises providing an aqueous alkali aluminate liquor having a temperature of 30 DEG to 100 DEG C. and containing 0.1 to 100 grams of Al2O3/l and 1 to 200 grams of Na2O/l, feeding in an alkali silicate solution within 10 to 200 minutes to obtain a clear reaction solution having the composition SiO2/Al2O3=50 to 1000:1, Na2O/SiO2=0.2 to 20:1 and H2O/Na2O=4 to 300:1, adding with stirring to the synthesis mixture an aqueous sodium aluminate which contains 10 to 200 grams/l of Al2O3 and 10 to 250 grams/l of Na2O having a temperature of 30 DEG to 100 DEG C. and allowing the thus obtained synthesis mixture to crystallize at a temperature within between 20 DEG and 175 DEG C. in at least 15 minutes.
专利号:US-4303626-A 优先权日:1976-11-11 标 题 :Crystalline zeolite powder of type A (V) 发明人:STRACK HANS; ROEBKE WOLFGANG; KNEITEL DIETER; PARR EHRFRIED 权利人:DEGUSSA; HENKEL KGAA 摘要:There are produced crystalline zeolite powders of Type A with the composition 1.0+/-0.2 M2/nO:Al2O3:1.85+/-0.5 SiO2xy H2O where M is a metal cation, n is its valence and y has a value up to 6 with 50 weight % of the particles not over 6.2 mu and with a particle spectrum: -Fraction ( mu ) Portion (weight %) -<3 10 to 60 -<5 25 to 95 -<10 80 to 99 -<15 92 to 100 - by hydrothermally crystallizing a SiO2, Al2O3, Na2O and water containing alkali aluminate, water, silicate synthesis mixture with an optional tempering step wherein in a given case during the crystallization or tempering step instead of stirring shearing forces are employed. The process comprises providing an aqueous alkali aluminate liquor having a temperature of 50 DEG to 90 DEG C. and containing 0.1 to 100 grams of Al2O3/l and 1 to 200 grams of Na2O/l, feeding in with stirring an alkali silicate solution, to the turbid reaction mixture having the composition SiO2/Al2O3=2 to 50:1, Na2O/SiO2=0.2 to 20:1 and H2O/NaO=4 to 300:1, adding with stirring in two steps an aqueous sodium aluminate which contains 10 to 200 grams/l ot Al2O3 and 10 to 250 grams/l of Na2O and having a temperature of 10 DEG to 100 DEG C. and allowing the thus obtained synthesis mixture to crystallize at a temperature within between 20 DEG and 175 DEG inside at least 15 minutes.
专利号:US-4303629-A 优先权日:1976-11-11 标题 :Crystalline zeolite powder of type A (I) 发明人:STRACK HANS; ROEBKE WOLFGANG; KNEITEL DIETER; PARR EHRFRIED 权利人:DEGUSSA; HENKEL KGAA 摘要:There are produced crystalline zeolite powders of Type A with the composition 1.0+/-0.2 M2/nO:Al2O3:1.85+/-0.5 SiO2xy H2O where M is a metal cation, n is its valence and y has a value up to 6 with 50 weight % of the particles not over 4.0 mu and with a particle spectrum -Fraction ( mu ) Portion (weight %) -<3 35 to 60 -<5 82 to 95 -<10 93 to 99 -<15 96 to 100 - by hydrothermally crystallizing a SiO2, Al2O3, Na2O and water containing alkali aluminate, water, silicate synthesis mixture with optionally a tempering step wherein in a given case during the crystallization or tempering step instead of stirring shearing forces are employed. The process comprises providing an aqueous alkali silicate soluton, heating it to a temperature between 30 DEG and 80 DEG C., adding to the alkali silicate solution with stirring aqueous sodium aluminate having a content of 0.1 to 100 grams Al2O3/l and 1 to 200 grams Na2O/l and which had been pre-warmed to from 30 DEG to 100 DEG C. until the turbidity point of the reaction mixture within 10 to 60 minutes, adding with stirring to the turbid reaction mixture which had the composition SiO2/Al2O3=2 to 50:1, Na2O/SiO2=0.2 to 20:1 and H2O/Na2O=4 to 300:1 an aqueous sodium aluminate which contains 10 to 200 grams/l of Al2O3 and 10 to 250 grams/l of Na2O having a temperature of 10 DEG to 100 DEG C. and allowing the thus obtained synthesis mixture to crystallize at a temperature within between 20 DEG and 175 DEG C. in at least 15 minutes.
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合成参考文献
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