CAS: 38136-75-3; L-Tryptophyl-L-Proline

相似化合物

251316-94-6 475101-70-3 1432793-75-3

📜Fmoc-Trp-Pro-Oh置于哌啶体系中,用 N,N-二甲基甲酰胺 作为反应溶剂,化学反应 0.5H,反应生成 色氨酰-脯氨酸
参考文献:Functional Identification And Structure Determination Of Two Novel Prolidases From Cog1228 In The Amidohydrolase Superfamily,
标题:Functional Identification And Structure Determination Of Two Novel Prolidases From Cog1228 In The Amidohydrolase Superfamily,
摘要:Two Uncharacterized Enzymes From The Amidohydrolase Superfamily Belonging To Cog1228 Were Cloned,Expressed,And Purified To Homogeneity. The Two Proteins,Sgx9260C (Gi|44242006) And Sgx9260B (Gi|44479596),Were Derived From Environmental Dna Samples Originating From The Sargasso Sea. The Catalytic Function And Substrate Profiles For Sgx9260C And Sgx9260B Were Determined Using A Comprehensive Library Of Dipeptides And N-Acyl Derivative Of L-Amino Acids. Sgx9260C Catalyzes The Hydrolysis Of Gly-L-Pro,L-Ala-L-Pro,And N-Acyl Derivatives Of L-Pro. The Best Substrate Identified To Date Is N-Acetyl-L-Pro With A Value Of K(Cat)/k-M Of 3 X 10(5) M-1 S(-1). Sgx9260B Catalyzes The Hydrolysis Of L-Hydrophobic L-Pro Dipeptides And N-Acyl Derivatives Of L-Pro. The Best Substrate Identified To Date Is N-Propionyl-L-Pro With A Value Of K(Cat)/k-M Of 1 X 10(5) M-1 S(-1). Three-Dimensional Structures Of Both Proteins Were Determined By X-Ray Diffraction Methods (Pdb Codes 3Mkv And 3Feq). These Proteins Fold As Distorted (Beta/alpha)(8)-Barrels With Two Divalent Cations In The Active Site. The Structure Of Sgx9260C Was Also Determined As A Complex With The N-Methylphosphonate Derivative Of L-Pro (Pdb Code 3N2C). In This Structure The Phosphonate Moiety Bridges The Binuclear Metal Center,And One Oxygen Atom Interacts With His-140. The A-Carboxylate Of The Inhibitor Interacts With Tyr-231. The Proline Side Chain occupies A Small Substrate Binding Cavity Formed By Residues Contributed From The Loop That Follows Beta-Strand 7 Within The (Beta/alpha)(8)-Barrel. A Total Of 38 Other Proteins From Cog1228 Are Predicted To Have The Same Substrate Profile Based On Conservation Of The Substrate Binding Residues. The Structure Of An Evolutionarily Related Protein,Cc2672 From Caulobacter Crecentus,Was Determined As A Complex With The N-Methylphosphonate Derivative Of L-Arginine (Pdb Code 3Mtw).
DOI:10.1021/bi100897U

专利信息

专利号:EP-2588602-A1
优先权日:2010-07-01
标题:Variants of glucoamylase
发明人：DEGN PETER EDVARD; BOTT RICHARD R; VROEMEN CASPER WILLEM; SCHEFFERS MARTIJN SILVAN; AEHLE WOLFGANG; PETERSEN ELIN
权利人:DUPONT NUTRITION BIOSCI APS; DANISCO US INC
摘要:The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DMA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

专利号:US-6383738-B1
优先权日:1996-08-30
标 题:Herpes simplex virus ORF P is a repressor of viral protein synthesis
发明人：BRUNI RENATO; ROIZMAN BERNARD
权利人:ARCH DEV CORP
摘要:The present invention is directed to methods and compositions relating to the treatment of herpes simplex virus infections and the screening of compounds for activity that inhibit or promote viral latency. The previously identified ORF P gene product now has been shown to interact with certain eukaryotic splicing factors and, in a cell infected with a herpesvirus containing a derepressed ORF P gene, ORF P can limit the splicing of at least two viral products. Given this function, it now is possible to screen for inhibitors and inducers of ORF P and, further, provide methods for maintaining and preventing viral latency.

专利号:CA-2394414-A1
优先权日:1999-12-16
标 题 :Moss genes from physcomitrella patens encoding proteins involved in the synthesis of tocopherols carotenoids

专利号:AT-512453-B1
优先权日:2010-10-22
标 题 :Improved synthesis of a biosynthetic product having an assembled composition of the biosynthetic enzymes on a nucleotide sequence motif

专利号:CA-2513289-A1
优先权日:2003-01-20
标 题:Expression cassette with promotors of starch synthesis 3 for the expression of nucleic acids in plant tissue containing starch

专利号:KR-102238183-B1
优先权日:2018-05-04
标 题 :Recombinant pangenotype complex NS1 antigen pool of dengue virus and endemic flaviviruses designed by gene synthesis