CAS: 993-13-5; Methylphosphonic Acid

上下游产品

methylphosphonic acid dichloroanhydride methylphosphine dibutyl methylphosphonate chloroacetic acid ethyl ester5,9]d°Cosane 3,7,11,15,18,21-hexaoxide1,5,9,13-Tetrahexyl-3,7,11,15,18,21-hexamethyl-2,4,6,8,10,12,14,16,17,19,20,22-dodecaoxa-3,7,11,15,18,21-hexaphospha-1,5,9,13-tetrasila-tricyclo[11.3.3.35,9]d°Cosane 3,7,11,15,18,21-hexaoxide Methylphosphonsaeure-bis-(methyl-diphenyl-silylester) Methylphosphonsaeure-bis-(aethyl-diphenyl-silylester) methylphosphonic acid bis(triphenylsilyl) ester

沙林置于水体系中,化学反应 672.0H,反应生成 甲基膦酸
参考文献:Mass Spectrometric Identification Of Methyl Phosphonic Acid: The Hydrolysis Product Of Isopropyl Methyl Phosphonofluoridate And Pinacolyl Methyl Phosphonofluoridate
标题:Mass Spectrometric Identification Of Methyl Phosphonic Acid: The Hydrolysis Product Of Isopropyl Methyl Phosphonofluoridate And Pinacolyl Methyl Phosphonofluoridate
摘要:
DOI:10.1021/ac00031A023

专利信息

专利号:US-6180347-B1
优先权日:1997-02-03
标 题:Method for monitoring transcriptional synthesis of RNA
发明人：IIDA YUKARI; KOSHIMOTO HIROYUKI; KONDO SATOSHI; TSUJI AKIHIKO
权利人:LAB MOLECULAR BIOPHOTONICS
摘要:This invention provides a method for monitoring the transcriptional synthesis of RNA, and to an apparatus therefor. Specifically, the invention resides in monitoring the initiation and termination of a transcription reaction for RNA synthesis, as well as the synthesis of full-length RNA by measuring the fluorescence of a pair of oligonucleotide probes of two types having a base sequence that continuously hybridizes to a part of a base sequence of the RNA synthesized by transcription, the pair of oligonucleotide probes comprising a donor probe labeled with an energy donor fluorescent molecule and an acceptor probe labeled with an energy acceptor fluorescent molecule.

专利号:US-8138330-B2
优先权日:2006-09-11
标 题 :Process for the synthesis of oligonucleotides
发明人：LEUCK MICHAEL; WOLTER ANDREAS; STUMPE ALFRED
权利人:LEUCK MICHAEL; WOLTER ANDREAS; STUMPE ALFRED; SIGMA ALDRICH CO LLC
摘要:The present invention discloses novel methods for the synthesis of oligonucleotides with nucleoside phosphoramidites on solid supports. The methods comprise the stepwise chain assembly of oligonucleotides on supports with 5′-acyl phosphoramidites. The synthesis cycles consist of a front end deprotection step which is conducted with a solution of a primary amine or a phenolate, a phosphoramidite coupling step with a 5′-acyl nucleoside phosphoramidite in the presence of an activator, a phosphite oxidation step and an optional capping step. The novel methods improve the quality of synthetic oligonucleotides due to the irreversibility of the front end deprotection step, which prevents the formation of deletion sequences, and due to the avoidance of acidic reagents in the synthesis cycles, which prevent the formation of depurination side products. The invention further discloses novel nucleoside phosphoramidite compositions wherein the phosphoramidites carry acyl front end protective groups which are cleavable with primary amines or phenolates. The invention is applicable to the synthesis of oligodeoxyribonucleotides, oligoribonucleotides and oligonucleotides with modifications in their sugar or phosphate groups.

专利号:US-7329515-B2
优先权日:2003-04-21
标题 :Solid support for the synthesis of 3′-amino oligonucleotides
发明人：LEUCK MICHAEL; WOLTER ANDREAS
权利人:SIGMA ALDRICH CO
摘要:The present invention discloses novel methods and solid supports for the synthesis of 3′-amino oligonucleotides. The novel supports are based on an unsubstituted or ring-substituted hydroxymethylbenzoyl linker element wherein the hydroxymethyl group is esterified to a solid phase bound carboxylic acid and the carbonyl group is linked to an amino alcohol as an amide. Oligonucleotides are conveniently synthesized on the novel supports with no modifications in the standard phosphoramidite synthesis scheme. The ester function of the support is cleaved under the alkaline deprotection conditions for oligonucleotides to provide a free hydroxymethyl group that aids in the release of the 3′-amino oligonucleotide products with a free amino group through neighbor group participation. The free amino group of the oligonucleotides is available for further conjugation reactions to haptens, reporter groups, surfaces or other small molecules or biomolecules. The methods provided are particularly mild, do not require any modifications in standard protocols for the synthesis and deprotection of oligonucleotides, provide the 3′-amino oligonucleotides free of side products and do not introduce chiral centers to the oligonucleotides.

专利号:US-5936080-A
优先权日:1996-05-24
标题 :Compositions and methods for the synthesis of organophosphorus derivatives
发明人：STEC WOJCIECH J; WOZNIAK LUCYNA
权利人:GENTA INC; POLSKA AKADEMIA NAUK INSTYTUT
摘要:New organophosphorus mononucleoside derivatives, methods of their synthesis and methods for the synthesis of organophosphorus dinucleotide derivatives utilizing the organophosphorus mononucleosides are provided. Synthesized organophosphorus mononucleoside derivatives of the present invention are characterized by the formula; ##STR1## Organophosphorus dinucleoside derivatives prepared from the synthesis methods provided in the present invention are characterized by the formula; ##STR2##

专利号:US-8193384-B2
优先权日:2005-07-29
标 题 :Polymer-bound phosphitylating reagents for the synthesis of organophosphorus compounds
发明人：PARANG KEYKAVOUS; AHAMDIBENI YOUSEF
权利人:PARANG KEYKAVOUS; AHAMDIBENI YOUSEF; RHODE ISLAND EDUCATION
摘要:The synthesis and biochemical utility of modified oligonucleotides containing diphosphodiester internucleotide linkages. The synthesis of these compounds was carried out using diphosphitylating reagents. Oligonucleotides containing diphosphate diester bridges wherein said oligonucleotides are synthesized via a solid-phase synthesis strategy to form modified oligonucleotides. Diphosphitylating, triphosphitylating, tetraphosphitylating, β-triphosphitylating, bifunctional diphosphitylating, bifunctional triphosphitylating, and bifunctional tetraphosphitylating reagents wherein, the phosphorus atoms are linked together through oxygen, sulfur, amino, or methylene groups and/or are substituted with chlorine, diisopropylamine and cyanoethoxy groups.

专利号:US-6531591-B1
优先权日:1999-07-07
标 题 :Synthesis of stable quinone and photoreactive ketone phosphoramidite reagents for solid phase synthesis of photoreactive-oligomer conjugates
发明人：FENSHOLDT JEF
权利人:EXIQON AS
摘要:Quinone phosphoramidite reagents as well as photoreactive ketone phosphoramidite reagents, such as anthraquinone phosphoramidite reagents and benzophenone phosphoramidite reagents were synthesized and used for the solid phase synthesis of photoreactive-oligonucleotide conjugates. These phosphoramidite reagents are stable, suitable for large-scale synthesis and designed for automated solid phase synthesis of oligomers terminating in a photoreactive moiety.