CAS: 3326-35-0; 3',6'-Dihydroxy-5-Nitro-3H-Spiro[isobenzofuran-1,9'-Xanthen]-3-One

上下游产品

CAS号610-27-5 4-硝基邻苯二甲酸 | CAS号108-46-3 间苯二酚 | CAS号5466-84-2 4-硝基邻苯二甲酸酐 | CAS号14926-29-5 5-硝基-二乙酸荧光素 | CAS号3326-34-9 5-氨基荧光素 | CAS号3326-32-7 异硫氰酸荧光素酯

📜4-硝基邻苯二甲酸置于sodium Hydroxide体系中,用 甲醇 作为反应溶剂,化学反应 2.0H,反应生成 4-硝基荧光素
参考文献:Fluorescent Human Ep3 Receptor Antagonists
标题:Fluorescent Human Ep3 Receptor Antagonists
摘要:Exchange Of The Lipophilc Part Of Ortho-Substituted Cinnamic Acid Lead Structures With Different Small Molecule Fluorophoric Moieties Via A Dimethylene Spacer Resulted In Hep(3)R Ligands With Affinities In The Nanomolar Concentration Range. Synthesized Compounds Emit Fluorescence In The Blue,Green,And Red Range Of Light And Have Been Tested Concerning Their Potential As A Pharmacological Tool. Hep(3)Rs Were Visualized By Confocal Laser Scanning Microscopy On Ht-29 Cells,On Murine Kidney Tissues,And On Human Brain Tissues And Functionally Were Characterized As Antagonists On Human Platelets. Inhibition Of Pge(2) And Collagen-Induced Platelet Aggregation Was Measured After Preincubation With Novel Hep(3)R Ligands. The Pyryllium-Labeled Ligand 8 Has Been Shown As One Of The Most Promising Structures,Displaying A Useful Fluorescence And Highly Affine Hep(3)R Antagonists.
DOI:10.1021/ml300191G

专利信息

专利号:WO-9509170-A1
优先权日:1993-09-28
标 题:Chemical synthesis of rhodamine derivatives
发明人：CORRIE JOHN EDGAR THOMAS; CRAIK JAMES STANLEY
权利人:MEDICAL RES COUNCIL; CORRIE JOHN EDGAR THOMAS; CRAIK JAMES STANLEY
摘要:A method for preparing rhodamine derivatives of formulae (a) or (b) or a mixture of such compounds, where X is bromo, chloro- or iodoacetamido, maleimido, or amino. These compounds are the 5 and 6 (or 5' and 6') isomers of the amine, haloacetamide and maleimide derivatives of tetramethylrhodamine. The method enables for the first time the production of isomerically pure compounds on a relatively large scale. The products are fluorescent and are useful for the labelling of proteins.