CAS: 15981-91-6; 2-Amino-5-Methylpyrimidin-4(3H)-One

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1402401-43-7 108-53-2 1292200-55-5

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CAS号107-31-3 甲酸甲酯 | CAS号50-01-1 盐酸胍 | CAS号105-37-3 丙酸乙酯 | CAS号4401-71-2 1,3,5-三甲基嘧啶-2,4... | CAS号113-00-8 guanidine | CAS号608-41-3 3-methylmalic acid | CAS号65-71-4 胸腺嘧啶 | CAS号20090-58-8 4-氯-5-甲基嘧啶-2-胺

📜2'-脱氧-5-甲基异胞苷置于盐酸体系中,用 水 用作溶剂,化学反应生成2-氨基-5-甲基-4-嘧啶醇
参考文献:含有5-甲基异胞嘧啶-鸟嘌呤和异鸟嘌呤-胞嘧啶碱基对的平行链寡核苷酸双链体
标题:含有5-甲基异胞嘧啶-鸟嘌呤和异鸟嘌呤-胞嘧啶碱基对的平行链寡核苷酸双链体
摘要:制备包含5-甲基异胞嘧啶-鸟嘌呤和/或异鸟嘌呤-胞嘧啶碱基对的平行链寡核苷酸.当2'-脱氧-5-甲基异胞苷(2A)和2'-脱氧异鸟苷(3)的亚磷酰胺用于固相合成时,偶联效率高于99%.在am衍生物4A,B的情况下,2'-脱氧-5-甲基异胞苷(1A)的糖基键对酸稳定.长时间暴露于氨中会发生脱嘧啶作用.使用the准分子荧光明确确定了平行链(ps)双链体的取向.反平行(aps)双链体d(a 12)•d(t 12)(25•26当两个中心的腺嘌呤-胸腺嘧啶碱基对被异鸟嘌呤-胞嘧啶对取代时,变为平行方向.发现包含5-甲基异胞嘧啶-鸟嘌呤碱基对的ps-双链体的热稳定性显着低于具有异鸟嘌呤-胞嘧啶对的ps-双链体的热稳定性.根据cd光谱,与aps对应物相比,Ps-Dna-Dna或dna-Rna杂种在二级结构上表现出差异.
Doi:10.1016/s0040-4020(99)00511-6

专利信息

专利号:US-12018251-B2
优先权日:2017-10-13
标 题:Method for synthesis of polynucleotides using a diverse library of oligonucleotides
发明人：VLADAR HAROLD PAUL; FERNANDES REDONDO RODRIGO APARECIDO
权利人:RIBBON BIOLABS GMBH
摘要:A method for synthesizing a target double stranded (ds) polynucleotide byproviding an oligonucleotide library within an array device that has a diversity of oligonucleotide library members, each of which has a different nucleotide sequence and is contained in a separate library containment in an aqueous solution. The library includes single stranded oligonucleotides and double stranded oligonucleotides with at least one overhang and covers at least 10,000 pairs of matching oligonucleotides. In a first step, at least a first pair of matching oligonucleotides are transferred transferred from the library into a first reaction containment using a liquid handler and the matching oligonucleotides are assembled, thereby obtaining a first reaction product comprising at least one overhang. Further reaction products are then likewise obtained and are assembled in a predetermined workflow using an algorithm, thereby producing said target ds polynucleotide with an overhang, optionally followed by a finalization step to prepare blunt ends.

专利号:US-2021171437-A1
优先权日:2017-07-17
标题 :Peptide nucleic acid (pna) monomers with an orthogonally protected ester moiety and novel intermediates and methods related thereto
发明人：COULL JAMES M; GILDEA BRIAN D
权利人:VERA THERAPEUTICS INC
摘要:The present disclosure pertains to peptide nucleic acid (PNA) monomers and oligomers, as well as methods and compositions useful for the preparation of PNA monomer precursors (e.g. PNA Monomer Esters, Backbone Esters and Backbone Ester Acid Salts, as described below) that can be used to prepare PNA monomers wherein said PNA monomers can be used to prepare said PNA oligomers. In some embodiments, the disclosure features sulfonic acid salts of Backbone Ester compounds, which sulfonic acid salts generally tend to be crystalline and can be obtained in reasonably good yield, often without requiring any chromatographic purification of the reaction product of the Backbone Ester synthesis reaction. This disclosure also pertains to novel methods for the synthesis of said Backbone Ester compounds and novel methods for the formation of the related sulfonic acid salts. Exemplary ester groups include, but are not limited to, 2,2,2-trichloroethy-(TCE), 2,2,2-tribromoethyl-(TBE), 2-iodoethyl-groups (2-IE) and 2-bromoethyl-(2-BrE) as the ester group. These particular ester groups can be removed under conditions where both Boc and Fmoc protected amine groups are stable.

专利号:US-2023317208-A1
优先权日:2022-03-31
标 题 :Biopolymer synthesis
发明人：VLADAR HAROLD P; AGOZZINO LUCA
权利人:RIBBON BIOLABS GMBH
摘要:The invention provides methods for synthesizing large biopolymers such as genome-scale polynucleotide molecules. Where genome-scale polynucleotides are to be made by attaching together a number of oligos, assembly of the desired polynucleotide from the oligos is represented as a tree, in which the branches represent nucleotide sequences and the nodes represent attachments between pairs of oligos. Numerous assembly trees are each generated and scored in silico according to how successful that tree will be in directing assembly of the desired polynucleotide, given biochemical properties of the oligos and proposed ligations. Systems and methods of the invention select a suitable tree and operate liquid handling systems to perform operations as shown by the selected tree to make the desired polynucleotide.

专利号:US-7211668-B2
优先权日:2003-07-28
标 题 :PNA monomer and precursor
发明人：KIM SUNG KEE; LEE HYUNIL; LIM JONG CHAN; LEE SUNG HEE
权利人:PANAGENE INC
摘要:This application relates to monomers of the general formula (I) for the preparation of PNA (peptide nucleic acid) oligomers and provides method for the synthesis of both predefined sequence PNA oligomers and random sequence PNA oligomers: n nwhereinn E is sulfur or oxygen; J is nitrogen or C—R′; R1, R′ is independently H, halogen, alkyl, nitro, nitrile, alkoxy, halogenated alkyl, halogenated alkoxy or a functional group having one of following general formulae: n nwhereinn A 1 , A 2 , A 3 , A 4 , A 5 , is independently selected from H, halogen such as F, Cl, Br or I, CF 3 , alkyl, preferably C 1 –C 4 alkyl, nitro, nitrile, alkoxy, preferably C 1 –C 4 alkoxy, halogenated (such as F, Cl, Br and I) alkyl, preferably halogenated C 1 –C 4 alkyl, halogenated (such as F, Cl, Br and I) alkoxy, preferably halogenated C 1 –C 4 alkoxy, phenyl, and halogenated (such as F, Cl, Br and I phenyl; R2 is H or protected or unprotected side chain of natural or unnatural α-amino acid; and B is a natural or unnatural nucleobase, wherein when said nucleobase has an exocyclic amino function, said function is protected by protecting group which is labile to acids but stable to weak to medium bases in the presence of thiol.

专利号:US-2007264694-A1
优先权日:2006-04-07
标题 :Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
发明人：PRUDENT JAMES R
权利人:ERAGEN BIOSCIENCES INC
摘要:The present methods relate to generating nucleic acid molecules using non-natural nucleotides. In some methods, the nucleic acid molecules may be generated by hybridizing a plurality of oligonucleotides comprising one or more non-natural nucleotides and using a polymerase and/or a coupling agent to link the hybridized oligonucleotides. The methods also relate to the use of proximity effects to generate nucleic acid molecules using non-natural nucleotides. Furthermore, the methods relate to the use of at least one non-natural base in a DNA template in order to generate a replicate of the DNA template in which the non-natural base has been replaced with a natural base.

专利号:US-7411065-B2
优先权日:2002-04-26
标 题 :PNA monomer and precursor
发明人：KIM SUNG KEE; LEE HYUNIL; LIM JONG CHAN; CHOI HOON; JEON JAE HOON; AHN SANG YOUL; LEE SUNG HEE; YOON WON JUN
权利人:PANAGENE INC
摘要:This application relates to monomers of the general formula (I) for the preparation of PNA (peptide nucleic acid) oglimers and provides method for the synthesis of both predefined sequence PNA oligomers and random sequence PNA oligomers: n nwhereinn E is nitrogen or C—R′; J is sulfur or oxygen; R′, R1, R2, R3, R4 is independently H, halogen, alkyl, nitro, nitrile, alkoxy, halogenated alkyl, halogenated alkoxy, phenyl or halogenated phenyl, R5 is H or protected or unprotected side chain of natural or unnatural α-amino acid; and B is a natural or unnatural nucleobase, wherein when said nucleobase has an exocyclic amino function, said function is protected by protecting group which is labile to acids but stable to weak to medium bases in the presence of thiol.