CAS: 122341-56-4; 3-(4'-Methoxyspiro[adamantane-2,3'-[1,2]Dioxetan]-4'-yl)Phenyl Dihydrogen Phosphate

专利信息

专利号:WO-2004113530-A1
优先权日:2003-06-18
标题:Polynucleotide for synthesis of labeled protein
发明人：NAKA DAIJI; NAKANO HIROSHI; SHIRATORI MIWA; KOBAYASHI TERUAKI; SUZUKI KATSUHIKO; HASHIMOTO HIDEMI; SASAKI TOORU
权利人:MITSUBISHI CHEM CORP; NAKA DAIJI; NAKANO HIROSHI; SHIRATORI MIWA; KOBAYASHI TERUAKI; SUZUKI KATSUHIKO; HASHIMOTO HIDEMI; SASAKI TOORU
摘要:A process for producing a labeled protein, comprising translating a gene template in the presence of a labeled compound having a label portion consisting of a labeled substance and an acceptor portion consisting of a compound capable of binding to the C-terminus of protein synthesized by a translation system. In particular, there are provided a polynucleotide for use in synthesis of labeled protein characterized by having the capability of enhancing labeling efficiency through addition to the 3’ end of a base sequence coding for target protein within the gene template and provided a process for producing a labeled protein that is carried out with the use of the polynucleotide.

专利号:US-6033881-A
优先权日:1995-06-13
标题:Method for one step isothermal non-transcription based amplification of nucleic acids
发明人：HIMMLER GOTTFRIED; SCHLEDERER THOMAS
摘要:PCT No. PCT/AT96/00106 Sec. 371 Date Apr. 6, 1998 Sec. 102(e) Date Apr. 6, 1998 PCT Filed Jun. 13, 1996 PCT Pub. No. WO97/00330 PCT Pub. Date Jan. 3, 1997The present invention relates to a process for the transcriptionless amplification of nucleic acids or nucleic acid sequences by means of enzymes in which the nucleic acids or sequences are divided at least partially into their individual strands before amplification and/or transcribed and the reaction mixture contains oligonucleotides with a base sequence essentially complementary to those of the ends of the nucleic acids or sequences to be amplified, where said oligonucleotides can, in certain conditions, form starting points and/or chemical components for the synthesis of nucleic acids and are built into the amplification product to be formed. The product of the components itself again corresponds to the nucleic acid or sequence to be amplified. The reaction product formed from the oligonucleotide components, possibly from the templates and other chemical components, reacts again in one step with the oligonucleotide components, the reaction being conducted substantially isothermally.

专利号:WO-2004083431-A1
优先权日:2003-03-19
标题:Method of screening protein capable of controlling transcription reaction and method of assaying activity thereof
发明人：KAWAI KUNJI; NAKA DAIJI; SUZUKI KATSUHIKO; NAKANO HIROFUMI; KONDO JUN
权利人:ZOEGENE CORP; KAWAI KUNJI; NAKA DAIJI; SUZUKI KATSUHIKO; NAKANO HIROFUMI; KONDO JUN
摘要:A method of screening a transcription associated factor characterized in that the synthesis of test protein is carried out in a cell-free protein synthetic system and the function of transcription associated factor is analyzed on the basis of, as an indicator, an interaction or change of interaction between the transcription associated factor and a DNA having a base sequence for transcription control region.

专利号:US-6124478-A
优先权日:1987-12-31
标题 :Methods of using 1,2-dioxetanes and kits therefore
发明人：BRONSTEIN IRENA Y; EDWARDS BROOKS
权利人:TROPIX INC
摘要:Compounds having the formula: ##STR1## wherein T is a polycycloalkylidene group (e.g., adamant-2-ylidene); R is a C 1-20 alkyl, aralkyl or cycloalkyl group; and Y is a fluorescent chromophore (e.g., m-phenylene), produced by reacting a compound having the formula: ##STR2## with an R-ylating agent (e.g., R 2 SO 4 ) in the presence of an alkali metal alkoxide in a polar aprotic solvent. Also, compounds having the formula: ##STR3## are produced by reacting a compound having the formula: ##STR4## with ##STR5## wherein X is an electronegative leaving group (e.g., a halogen anion such as chloride ion) in the presence of a Lewis base (e.g., a trialkyl-amine) dissolved in an aprotic organic solvent (e.g., benzene or toluene). Also, compounds having the formula ##STR6## are produced by reacting a compound of the formula ##STR7## with a tetra-O-acylated-O-hexopyranoside halide, then hydrolyzing off the protective acyl groups. The aforementioned compounds and procedures are useful in the synthesis of enzyme-cleavable 1,2-dioxetane ring systems that can serve as members of a binding pair employed, for example, in chemiluminescent immunoassays, DNA probe assays, and direct assays for an enzyme.

专利号:US-5362916-A
优先权日:1989-09-06
标题 :Synthesis of mercaptaryl or hydroxyaryl enol ether alkali metal salts
发明人：EDWARDS BROOKS; JUO ROUH-RONG
权利人:TROPIX INC
摘要:Hydroxyaryl enol ether alkali metal salts having the formula: ##STR1## in which T is a fused, substituted or unsubstituted polycycloalkylidene group, OR 3 is an ether group Y is a light-emitting fluorophore-forming group which will be part of a luminescent substance formed by decomposition of a 1,2-dioxetane subsequently formed from the hydroxyaryl enol ether alkali metal salt, capable of absorbing energy to form an excited state from which it emits optically detectable energy to return to its ground state, and AM 30 is an alkali metal cation, processes for the preparation of these intermediate salts, and their use as starting materials for acylation, phosphorylation and glycosylation reactions to give intermediates reactable to give stable, water-soluble chemiluminescent 1,2-dioketames, particularly ones that are enzymatically cleavable, are disclosed.

专利号:US-7964360-B2
优先权日:2002-11-08
标 题 :Uncoupled collagen synthesis and degradation assays
发明人：SANDELL LINDA J; GARNERO PATRICK
权利人:BARNES JEWISH HOSPITAL
摘要:The invention relates to methods for determining the progression of cartilage degeneration diseases, such as osteoarthritis and rheumatoid arthritis, by quantitating collagen synthesis and degradation markers in patient samples. One can determine whether a cartilage degeneration condition is progressing, regressing, or remaining stable by quantitating collagen synthesis and degradation markers in patient samples and comparing the value obtained to a reference value. When a joint affected by cartilage degeneration in question expresses collagen synthesis and degradation markers, a change in this value is indicative of a change in the progression of the cartilage degeneration condition. The methods and apparatus of the invention allow accurate determination of the therapeutic effects certain cartilage degeneration drug treatments, including osteoarthritis and rheumatoid arthritis drug treatments, so are also useful for pharmaceutical efficacy studies in mammals.