专利号:US-2007174933-A1 优先权日:2006-01-25 标题 :Altering levels of anti-nutrient factors in plants 发明人:HANNOUFA ABDELALI; SCHAFER ULRIKE A 权利人:HANNOUFA ABDELALI; SCHAFER ULRIKE A 摘要:A method for reducing the level of one or more than one protein in a plant or a tissue within the plant, and plants with reduced levels of target proteins are provided. The method involves introducing a nucleic acid sequence into the plant, where the nucleic acid sequence comprises a regulatory region operatively associated with a silencing nucleotide sequence. The expression of the silencing nucleotide sequence reduces or eliminates the expression of two or more than two enzymes involved in the synthesis of the one or more than one protein. The reduced level of the one or more than one protein may be determine by comparing the level of the one or more than one protein in the plant, or a tissue of the plant, with a level of the one ore more than one protein in a second plant, or the tissue from the second plant, that does not express the nucleic acid sequence. Plants and seeds obtained from plants with reduced levels of target proteins, and reduced anti-nutrient factors are also provided
专利号:WO-2004101755-A3 优先权日:2003-05-07 标 题:Ref1 modified plants and plant seeds 发明人:CHAPPLE CLINT; NAIR RAMESH 权利人:PURDUE RESEARCH FOUNDATION; CHAPPLE CLINT; NAIR RAMESH 摘要:The present invention relates to plant cell wall synthesis, and to the synthesis of soluble compounds in plants commonly known as secondary metabolites or natural products. In particular, the present invention provides plant aldehyde dehydrogenases with a novel capacity of oxidizing hydroxycinnamaldehydes to hydroxycinnamic acids, and genes encoding the enzyme, as well as variants of the gene, and methods of using the genes and variants of the gene to modify plant chemistry. The methods of the present invention include reducing sinapine content of a seed, decreasing hydroxycinnamic acid content in a plant cell, and modifying lignin property in a plant cell wall by manipulating the activity of the novel plant aldehyde dehydrogenase in a plant cell.
专利号:US-8614369-B2 优先权日:2007-06-25 标 题:Enzymes involved in triterpene synthesis 发明人:OSBOURN ANNE; QI XIAOQUAN 权利人:PLANT BIOSCIENCE LTD 摘要:This invention relates to isolated polynucleotides encoding enzymes consisting of a carboxypeptidase-like protein, a methyltransferase and a glucosyltransferase, involved in the biosynthesis of β-amyrin-derived triterpenes in plants and seeds. The invention also relates to the construction of recombinant DNA constructs comprising all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to at least one regulatory sequence.
专利号:EP-4100388-A1 优先权日:2020-02-07 标题 :New pathway for the synthesis of sinapoyl malate, of sinapine and of analogues thereof, and use thereof as antimicrobial molecules
专利号:WO-2021156578-A1 优先权日:2020-02-07 标题:New pathway for the synthesis of sinapoyl malate, of sinapine and of analogues thereof, and use thereof as antimicrobial molecules
专利号:US-2011296543-A1 优先权日:2006-06-01 标题:Nucleic acids and proteins and methods for making and using them 发明人:CHANG HWAI; MATHUR ERIC J; CAYOUETTE MICHELLE; ROBERTSON DAN E; HUGENHOLTZ PHILIP; WARNECKE FALK; LEADBETTER JARED R; IVANOVA NATALIA; LUGINBUHL PETER; HUTCHISON DON 权利人:CHANG HWAI; MATHUR ERIC J; CAYOUETTE MICHELLE; ROBERTSON DAN E; HUGENHOLTZ PHILIP; WARNECKE FALK; LEADBETTER JARED R; IVANOVA NATALIA; LUGINBUHL PETER; HUTCHISON DON; UNIV CALIFORNIA; CALIFORNIA INST OF TECHN; VERENIUM CORP 摘要:The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. Polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to a biofuel (e.g., a bioethanol, biomethanol, biopropanol or biobutanol, a biodiesel, etc.), biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.
1: Guo Y, An H, Feng L, Liu Q, Wang S, Zhang T. Sinapine as an active compound for inhibiting the proliferation of Caco-2 cells via downregulation of P-glycoprotein. Food Chem Toxicol. 2014 May;67:187-92. doi: 10.1016/j.fct.2014.02.035. Epub 2014 Mar 5. doi: 10.1371/journal.pone.0116470. eCollection 2015. 3: Zhou H, Huang Y, Hoshi T, Kashiwagi Y, Anzai J, Li G. Electrochemistry of sinapine and its detection in medicinal plants. Anal Bioanal Chem. 2005 Jun;382(4):1196-201. Epub 2005 May 20. Epub 2007 Nov 23. doi: 10.1007/s11248-008-9194-3. Epub 2008 Jul 9. doi: 10.1104/pp.110.169821. Epub 2011 Jan 19. 8: Yang M, Zheng C, Zhou Q, Liu C, Li W, Huang F. Influence of microwaves treatment of rapeseed on phenolic compounds and canolol content. J Agric Food Chem. 2014 Feb 26;62(8):1956-63. doi: 10.1021/jf4054287. Epub 2014 Feb 11. doi: 10.1007/s00122-011-1760-z. Epub 2011 Dec 24. doi: 10.1371/journal.pone.0182747. eCollection 2017.
合成参考文献
参考文献:10.1038/s41467-021-24482-1 摘要:Wu P, Chen D, Ding W, Wu P, Hou H, Bai Y, Zhou Y, Li K, Xiang S, Liu P, Ju J, Guo E, Liu J, Yang B, Fan J, He L, Sun Z, Feng L, Wang J, Wu T, Wang H, Cheng J, Xing H, Meng Y, Li Y, Zhang Y, Luo H, Xie G, Lan X, Tao Y, Li J, Yuan H, Huang K, Sun W, Qian X, Li Z, Huang M, Ding P, Wang H, Qiu J, Wang F, Wang S, Zhu J, Ding X, Chai C, Liang L, Wang X, Luo L, Sun Y, Yang Y, Zhuang Z, Li T, Tian L, Zhang S, Zhu L, Chang A, Chen L, Wu Y, Ma X, Chen F, Ren Y, Xu X, Liu S, Wang J, Yang H, Wang L, Sun C, Ma D, Jin X, Chen G. The trans-omics landscape of COVID-19. Nature Communications. 2021 Jul 27;12(1):4543. doi: 10.1038/s41467-021-24482-1. 参考文献:10.1016/j.plaphy.2008.04.014 摘要:Huang J, Rozwadowski K, Bhinu VS, Schäfer U, Hannoufa A. Manipulation of sinapine, choline and betaine accumulation in Arabidopsis seed: towards improving the nutritional value of the meal and enhancing the seedling performance under environmental stresses in oilseed crops. Plant Physiol Biochem. 2008 Jul;46(7):647–54. doi: 10.1016/j.plaphy.2008.04.014.