CAS: 114041-00-8; Diasp

专利信息

专利号:US-2025115956-A1
优先权日:2021-06-23
标题 :Methods and compositions for nucleic acid sequencing
发明人：WHITEFORD NAVA EDMOND
权利人:Nanogami GmbH
摘要:The present disclosure in some aspects relates to real-time nucleic acid sequencing comprising one or more detectable label deactivation steps, such as stochastic photobleaching that occurs during sequential nucleotide incorporation in a sequencing-by-synthesis reaction. Deactivation of the detectable label(s) of a particular labeled nucleotide may occur prior to, during, and/or after the incorporation of the next nucleotide, for instance, a labeled nucleotide that forms a phosphodiester bond with the particular labeled nucleotide. Also described herein are methods of analyzing sequencing data obtained from the sequencing methods.

专利号:US-2025109436-A1
优先权日:2022-02-08
标 题 :Methods, compositions, and systems for long read single molecule sequencing
发明人：WHITEFORD NAVA EDMOND
权利人:RETICULA INC
摘要:The present disclosure in some aspects relates to real-time nucleic acid sequencing comprising i) cluster decimation by deactivating some strands in a cluster, ii) bringing the non-deactivated strands in the cluster out of sync with each other during sequencing, and/or iii) one or more detectable label deactivation steps, such as stochastic photobleaching that occurs during sequential nucleotide incorporation in a sequencing-by-synthesis reaction. Deactivation of the detectable label(s) of a particular labeled nucleotide may occur prior to, during, and/or after the incorporation of the next nucleotide, for instance, a labeled nucleotide that forms a phosphodiester bond with the particular labeled nucleotide. Also described herein are methods of analyzing sequencing data obtained from the sequencing methods.