专利号:US-7344863-B2 优先权日:1998-03-13 标题:Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules 发明人:LI WU-BO; JESSEE JOEL A; SCHUSTER DAVID; XIA JIULIN; GEBEYEHU GULILAT 权利人:INVITROGEN CORP 摘要:The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.
专利号:US-6030814-A 优先权日:1997-04-21 标题:Reverse transcription method 发明人:JENDRISAK JEROME J 权利人:EPICENTRE TECHNOLOGIES CORP 摘要:A method of improving the synthesis of full-length cDNA transcripts by Mn ++ -dependent reverse transcriptases, preferably DNA-dependent DNA polymerases, is disclosed.
专利号:WO-9946400-A1 优先权日:1998-03-13 标题:Compositions and methods for enhanced synthesis of nucleic acid molecules
专利号:EP-1062360-A1 优先权日:1998-03-13 标 题 :Compositions and methods for enhanced synthesis of nucleic acid molecules
专利号:US-2004265969-A1 优先权日:1998-03-13 标 题 :Compositions and methods for enhanced synthesis of nucleic acid molecules
专利号:EP-1062360-B1 优先权日:1998-03-13 标 题:Compositions and methods for enhanced synthesis of nucleic acid molecules
[参考文献]: Vladimir V Dynnik, Et Al. To Break Or To Brake Neuronal Network Accelerated By Ammonium Ions. Plos One. 2015 Jul 28;10(7):E0134145. [参考文献]: Yulia Kaluzhny, Et Al. The Epiocular Eye Irritation Test (Eit) For Hazard Identification And Labelling Of Eye Irritating Chemicals: Protocol Optimisation For Solid Materials And The Results After Extended Shipment. Altern Lab Anim. 2015 May;43(2):101-27.
合成参考文献
参考文献:10.1385/1-59745-005-7:285 摘要:Tagoh H, Cockerill PN, Bonifer C. In vivo genomic footprinting using LM-PCR methods. Methods Mol Biol. 2006;325():285–314. doi: 10.1385/1-59745-005-7:285. 参考文献:10.1023/b:bmmd.0000031750.37323.dd 摘要:Gutmann O, Kuehlewein R, Reinbold S, Niekrawietz R, Steinert CP, de Heij B, Zengerle R, Daub M. A highly parallel nanoliter dispenser for microarray fabrication. Biomed Microdevices. 2004 Jun;6(2):131–7. doi: 10.1023/b:bmmd.0000031750.37323.dd.