专利号:US-6228574-B1 优先权日:1998-11-17 标题 :Analytical system 发明人:ROTMAN M BORIS 摘要:An analytical system for rapid detection and identification of different analytes directly from a test sample by mixing test material with a germinogenic source and enzyme-free spores, allowing the mixture to stand for a time to allow analyte-induced spore germination and subsequent de novo synthesis of an enzyme capable of producing a germinant in the presence of the germinogenic source and detecting the presence of a germination-derived product. The germinant which is formed promotes further spore germination with concomitant additional de novo enzyme synthesis which results in a propagating cascade of analyte-independent germination after which a germination-derived product can be easily detected. The technique is particularly efficient to conduct thousands of parallel assays in an array of microscopic wells.
专利号:US-11293060-B2 优先权日:2017-04-12 标 题 :Method for labeling of aldehyde containing target molecules 发明人:BERGMANN FRANK; CHAU IAN QUANG; CRISALLI PETER; KALLEWAARD-LUM HANNAH MARIE; KUCHELMEISTER HANNES; POMPLUN SEBASTIAN JOHANNES; SAQIB HASHAM; WELLNER CHRISTIAN 权利人:ROCHE SEQUENCING SOLUTIONS INC 摘要:The present invention relates to improving the processing rate of a sequencing reaction, for example in a nanopore sequencing reaction, by means of using improved nucleoside-tags. The tags are linked to the nucleoside phosphate via a Pictet Spengler reaction. Exemplary sequencing reactions that are improved by the present methods include nanopore-based nucleic acid sequencing-by-synthesis reactions.
专利号:US-6872539-B2 优先权日:1998-11-17 标 题:Analytical system based upon spore germination 发明人:ROTMAN M BORIS 摘要:An analytical system for rapid detection and identification of different analytes directly from a test sample by mixing test material with a germinogenic source and enzyme-free spores, allowing the mixture to stand for a time to allow analyte-induced spore germination and subsequent de novo activity of an enzyme capable of producing a germinant in the presence of the germinogenic source and detecting the presence of a germination-derived product. The germinant which is formed promotes further spore germination with concomitant additional de novo enzyme synthesis or activation which results in a propagating cascade of analyte-independent germination after which a germination-derived product can be easily detected. The technique is particularly efficient to conduct thousands of parallel assays in an array of microscopic wells.
参考文献:10.1111/j.1399-3011.1989.tb00689.x 摘要:RAMASUBBU N, PARTHASARATHY R. Crystal structure and conformation of l‐pyroglutamyl‐l‐alanine. International Journal of Peptide and Protein Research. 1989 May;33(5):328–34. doi: 10.1111/j.1399-3011.1989.tb00689.x.