CAS: 132523-91-2; [2-[2-[2-[bis(Carboxymethyl)Amino]-5-Methylphenoxy]Ethoxy]-4-[3,6-Bis(Dimethylamino)Xanthen-9-Ylidene]Cyclohexa-2,5-Dien-1-Ylidene]-Bis(Carboxymethyl)Azanium,Chloride

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      专利号:WO-2025007089-A1
      优先权日:2023-06-30
      标 题:Compositions, methods and kits for nucleic acids synthesis and amplification
      发明人:BERKMAN JENNIFER; URBONAS IGNAS; ABREU DANIELA CRUZ; LIU CHENGJING; YEN CHIA AN; ONG KIMBERLIE SOOHOO; WEI WEI; GOMEZ SILVAN MARIA CINTA
      权利人:LIFE TECHNOLOGIES CORP; THERMO FISHER SCIENTIFIC BALTICS UAB
      摘要:The present disclosure is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in an one-step qPCR or RT-qPCR procedure comprising a reverse transcriptase, a DNA polymerase, at least one dNTP, and at least one stabilizing agent, wherein said at least one stabilizing agent increases stability of an assembled polymerase chain reaction.

      专利号:US-12006540-B2
      优先权日:2015-09-28
      标题:Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis
      发明人:JU JINGYUE; LI XIAOXU; CHEN XIN; LI ZENGMIN; KUMAR SHIV; SHI SHUNDI; GUO CHENG; REN JIANYI; HSIEH MIN-KANG; CHIEN MINCHEN; TAO CHUANJUAN; ERTURK ECE; KALACHIKOV SERGEY; RUSSO JAMES J
      权利人:UNIV COLUMBIA
      摘要:Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.

      专利号:US-9005895-B2
      优先权日:2010-06-21
      标题:Compositions, methods and kits for nucleic acid synthesis and amplification
      发明人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W
      权利人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W; LIFE TECHNOLOGIES CORP
      摘要:The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.

      专利号:US-11959137-B2
      优先权日:2015-09-28
      标题:Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis

      专利号:US-2024376536-A1
      优先权日:2015-09-28
      标题 :Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis

      专利号:US-2022411860-A1
      优先权日:2015-09-28
      标题:Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis

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      合成参考文献


      参考文献:10.1054/ceca.2001.0264
      摘要:Pitter JG, Maechler P, Wollheim CB, Spät A. Mitochondria respond to Ca2+ already in the submicromolar range: correlation with redox state. Cell Calcium. 2002 Feb;31(2):97–104. doi: 10.1054/ceca.2001.0264.
      参考文献:10.1046/j.1365-2982.2002.00319.x
      摘要:Rich A, Hanani M, Ermilov LG, Malysz J, Belzer V, Szurszewski JH, Farrugia G. Physiological study of interstitial cells of Cajal identified by vital staining. Neurogastroenterol Motil. 2002 Apr;14(2):189–96. doi: 10.1046/j.1365-2982.2002.00319.x.
      参考文献:10.1152/ajpheart.00123.2003
      摘要:Omichi C, Lamp ST, Lin S, Yang J, Baher A, Zhou S, Attin M, Lee M, Karagueuzian HS, Kogan B, Qu Z, Garfinkel A, Chen P, Weiss JN. Intracellular Ca dynamics in ventricular fibrillation. American Journal of Physiology-Heart and Circulatory Physiology. 2004 May;286(5):H1836–44. doi: 10.1152/ajpheart.00123.2003.
      参考文献:10.1002/mrd.1080320108
      摘要:Storey BT, Hourani CL, Kim JB. A transient rise in intracellular Ca2+ is a precursor reaction to the zona pellucida‐induced acrosome reaction in mouse sperm and is blocked by the induced acrosome reaction inhibitor 3‐quinuclidinyl benzilate. Molecular Reproduction Devel. 1992 May;32(1):41–50. doi: 10.1002/mrd.1080320108.
      参考文献:10.1016/s0006-3495(02)75194-1
      摘要:Brandes R, Bers DM. Simultaneous Measurements of Mitochondrial NADH and Ca2+ during Increased Work in Intact Rat Heart Trabeculae. Biophysical Journal. 2002 Aug;83(2):587–604. doi: 10.1016/s0006-3495(02)75194-1.
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