专利号:US-2025019732-A1 优先权日:2023-07-07 标 题:Method for chemical-biological cascade synthesis of l-phosphinothricin and mutants therefor 发明人:XUE YAPING; CHENG FENG; ZOU SHUPING; XU JIANMIAO; ZHENG YUGUO 权利人:UNIV ZHEJIANG TECHNOLOGY 摘要:A method for chemical-biological cascade synthesis of L-phosphinothricin is carried out as follows: 3-(methylethoxyphosphinyl) ethyl propionate is synthesized by addition reaction from diethoxymethylphosphine and acrylic acid, then a condensation reaction is carried out with 3-(methylethoxyphosphinyl) ethyl propionate and sodium ethoxide as reactants, then the product is subjected to a hydrolysis reaction with diethyl oxalate to synthesize 4-(hydroxymethylphosphinyl)-2-oxobutyric acid, and finally, L-phosphinothricin is catalytically synthesized by taking 4-(hydroxymethylphosphinyl)-2-oxobutyric acid as a raw material, and using highly active and stable wet cells co-expressing phsophinothricin dehydrogenase and alcohol dehydrogenase or co-expressing a phsophinothricin dehydrogenase mutant and alcohol dehydrogenase as a biocatalyst, thereby solving the problems of existing L-phosphinothricin synthesis being tedious, low asymmetric amination reduction activity and poor stability.
专利号:WO-2021125500-A1 优先权日:2019-12-17 标题 :Os12bglu38 gene for induction of male sterility in rice and use thereof 发明人:JEON JONG-SEONG; MAHONG BANCHA; LEE SANG-KYU; SHIM SU-HYEON 权利人:UNIV INDUSTRY COOPERATION GROUP KYUNG HEE UNIV 摘要:The present invention relates to Oryza sativa beta-glucosidase 38 (Os12BGlu38) gene, which is predominantly expressed in the late phase of pollen development to regulate the synthesis of intine in pollen cell walls, and a use thereof. The present invention relates to Os12BGlu38 gene, which regulates the synthesis of intine in pollen cell walls to ultimately induce male sterility in rice, a protein thereof, a dysfunctional mutant, a transgenic plant and a seed thereof, a method for regulating synthesis of intine in pollen cell walls of plants by expressing the gene in the plants, a method for inducing male sterility, and a pharmaceutical composition for induction of male sterility in plants.
专利号:US-7247456-B2 优先权日:1998-01-15 标题:Preparation of fucosylated oligosaccharides 发明人:SJOBERG ERIC R 权利人:NEOSE TECHNOLOGIES INC 摘要:This invention provides methods for practical enzymatic conversion of GDP-mannose to GDP-fucose. These methods are useful for efficient synthesis of reactants used in the synthesis of fucosylated oligosaccharides.
专利号:US-9181559-B2 优先权日:2009-09-15 标题 :Generation of high polyhydroxybutyrate producing oilseeds 发明人:PATTERSON NII; TANG JIHONG; HAN JIXIANG; TAVVA VENKATA; HERTIG ANDREW; ZHANG ZHIGANG; RAMSEIER THOMAS MARTIN; BOHMERT-TATAREV KAREN; PEOPLES OLIVER P; SNELL KRISTI D 权利人:PATTERSON NII; TANG JIHONG; HAN JIXIANG; TAVVA VENKATA; HERTIG ANDREW; ZHANG ZHIGANG; RAMSEIER THOMAS MARTIN; BOHMERT-TATAREV KAREN; PEOPLES OLIVER P; SNELL KRISTI D; METABOLIX INC 摘要:Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates (“PHAâ€?) are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate (“PHBâ€?). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.
专利号:US-12031160-B2 优先权日:2018-12-28 标 题:Method for the production of glufosinate-ammonium 发明人:XUE YAPING; CHENG FENG; LI QINGHUA; ZHENG YUGUO; XU JIANMIAO 权利人:UNIV ZHEJIANG TECHNOLOGY 摘要:The present invention provides a glufosinate-ammonium dehydrogenase mutant and application in synthesis of L-glufosinate-ammonium thereof, the method uses 2-carbonyl-4-[(hydroxy)(methyl)phosphinoyl]-butyric acid or its salts as a substrate and the glufosinate-ammonium dehydrogenase or cells containing the glufosinate-ammonium dehydrogenase as a biocatalyst to carry out reductive amination, thereby obtaining L-glufosinate-ammonium. The method has features of high conversion rate of raw materials, high yield, easy separation and purification of the product, and high chiral purity; compared with other catalytic processes, the method in the present invention has features of relatively simple process and a conversion rate of raw materials up to 100%.
专利号:WO-2024256370-A1 优先权日:2023-06-13 标 题 :Synthesis of glufosinate using an amidase-based process 发明人:ZIMMERMANN GUNTHER; POTT MORITZ STEFAN 权利人:BASF SE 摘要:The present invention relates to a method of preparing glufosinate, comprising the steps of hydrolysing an N-carbamoyl glufosinate amide with an Amidase enzyme to form an N- carbamoyl amino acid compound followed by cleaving off the carbamoyl moiety of said N- carbamoyl amino acid compound.
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合成参考文献
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