📜N-苄氧羰基-L-酪氨酸置于1,4-二氧六环,三正丁胺,氯甲酸乙酯体系中,化学反应生成 N-苄氧羰基-L-酪氨酸对硝基苯酯 参考文献:Martin Et Al.,Journal Of Biological Chemistry,1959,Vol. 234,P. 294,295 标题:Martin Et Al.,Journal Of Biological Chemistry,1959,Vol. 234,P. 294,295
专利号:US-5116741-A 优先权日:1988-04-12 标题 :Biosynthetic uses of thermostable proteases 发明人:BRYAN PHILIP N; PANTOLIANO MICHAEL W; ROLLENCE MICHELE L; WONG CHI H 权利人:GENEX CORP 摘要:The invention relates to the novel use of mutants of subtilisin in organic syntheses reactions in non-native environments. Especially the invention relates to methods for the use of mutant subtilisins in organic solvents for the catalysis of reactions involving ester formation and cleavage, including acylations and deacylations, and amidations and deamidations. The methods provide novel strategies which are useful in the synthesis of deoxynucleosides, dideoxynucleosides, peptides, sugars and the like.
专利号:US-5304631-A 优先权日:1990-01-16 标题:Synthetic helizyme enzymes 发明人:STEWART JOHN M; HAHN KARL W; KLIS WIESLAW A 权利人:UNIV COLORADO FOUNDATION 摘要:The design, and synthesis of peptide-based molecules termed helizymes which possess catalytic activity are described herein The catalytic molecules of the invention comprise a number of amphiphilic helical peptides, which interact via hydrophobic interactions and which are bonded at their carboxyl ends to a multifunctional base. Active site residues functional for catalysis are positioned within or at the N-termini of the helical peptides. The helizyme molecule adopts a conformation in aqueous medium such that a substrate binding pocket is formed and such that functional active site geometry results from the association of the helical peptides Specifically exemplified helizymes possess specificities and at least one catalytic activity of chymotrypsin, trypsin and acetylcholine esterase.