专利号:US-6960645-B2 优先权日:2004-03-26 标 题:Synthesis of ion imprinted polymer particles 发明人:RAMAKRISHNAN KALA; JOSEPH MARY G; RAO TALASILA P 权利人:COUNCIL SCIENT IND RES 摘要:Ion imprinted polymer materials are synthesized containing metal ion recognition sites. These particles are synthesized by copolymerizing with functional and cross linking monomers in presence of at least one imprint metal ion in the form of ternary complex. The polymerization was carried out by â–¡-irradiation (in the absence of initiator) or photochemical and thermal polymerization (in presence of initiator, AIBN). These materials were ground and sieved after drying to obtain erbium ion imprinted polymer particles. The erbium ion was removed from the polymer particles by leaching with mineral acid which leaves cavities/binding sites in the polymer particles. The resultant polymer particles can be used as solid phase extractants for selective enrichment of erbium ions from dilute aqueous solutions.
专利号:WO-0026406-A1 优先权日:1998-11-04 标题:METHOD FOR INDEXING AND DETERMINING THE RELATIVE CONCENTRATION OF EXPRESSED MESSENGER RNAs 发明人:HASEL KARL W; HILBUSH BRIAN S 权利人:DIGITAL GENE TECH INC 摘要:An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA and performing two sequence specific PCR amplifications of the cDNA. In preferred embodiments, the method comprises comparing the length and at least part of the nucleotide sequence of the PCR products to expected values determined from a database of nucleotide sequences. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions. Also provided are vectors and primers useful for the practice of the improved method.
专利号:CN-101418062-A 优先权日:2003-12-31 标题:Synthesis of Ionically Imprinted Polymer Particles
专利号:US-2005215762-A1 优先权日:2004-03-26 标题:Synthesis of ion imprinted polymer particles
专利号:WO-2005063382-A1 优先权日:2003-12-31 标题:Synthesis of ion imprinted polymer particles
专利号:JP-2007518545-A 优先权日:2003-12-31 标 题 :Synthesis of ion-imprinted polymer particles
1: Johnson ML. Fluorescence with chloroxine shampoo. Arch Dermatol. 1979 May;115(5):641. Italian. Italian.
合成参考文献
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