CAS: 6014-42-2; (2R,3R,4R,5R,6S)-6-Methyltetrahydro-2H-Pyran-2,3,4,5-Tetraol

上下游产品

CAS号513-86-0 3-羟基-2-丁酮 | CAS号513-85-9 2,3-丁二醇 | CAS号78-98-8 丙酮醛 | CAS号822-36-6 4-甲基咪唑 | CAS号930-62-1 2,4-二甲基咪唑 | CAS号6748-70-5 L-鼠李糖缩二乙硫醇 | CAS号620-02-0 5-甲基-2-糠醛 | CAS号14133-63-2 甲基2,3-O-异丙叉-α-L... | CAS号110-15-6 琥珀酸； 丁二酸

📜芦丁置于hesperidin-α-1,6-Rhamnosidase,水体系中,化学反应 30.0H,反应生成 L-(+)-鼠李糖
参考文献:Selective Hydrolysis By Commercially Available Hesperidinase For Isoquercitrin Production
标题:Selective Hydrolysis By Commercially Available Hesperidinase For Isoquercitrin Production
摘要:Isoquercitrin Is A Rare Flavonol Glycoside With Various Biological Activities,And It Is A Primary Synthetic Precursor For Enzymatically Modified Isoquercitrin (Emiq),Which Was Recently Approved As A Multiple Food Additive. The Enzymatic Method Has A Great Potential To Produce Isoquercitrin From Hydrolysis Of Rutin Via Selectively Removing A Terminal Rhamnose. Commercially Available Hesperidinase Was Investigated And Found That Alpha-L-Rhamnosidase Achieves Good Catalytic Capacity While Beta-D-Glucosidase Loses Its Activity At Ph 7.0. The Increased Conversion Of Rutin To Isoquercitrin Was Found By The Addition Of Some Metal Ions (K+,Li+,Mg2+,Zn2+ And Al3+). Electrospray Ionization Mass Spectrometry (Esi-Ms) And Nuclear Magnetic Resonance (NMR) Were Used To Identify Isoquercitrin From The Transformed Products. The Enzymatic Reaction Conditions (Temperature,Substrate Concentration,And Reaction Time) Of Isoquercitrin From Rutin Were Optimized,And The Apparent Kinetic Parameters V-M/k-M At Different Temperatures Were Also Measured. The Results Suggest That The Commercially Available Hesperidinase Can Be Applied Into Isoquercitrin Production Via Highly Selective Hydrolysis. (C) 2012 Elsevier B.V. All Rights Reserved.
DOI:10.1016/j.Molcatb.2012.05.005