专利号:US-2004171822-A1 优先权日:2001-01-12 标题:Methods for determining ring number in carotenoids by lycopene epsilon-cyclasses and uses thereof 发明人:CUNNINGHAM JR FRANCIS X; GANTT ELISABETH 摘要:The invention relates to methods for mapping and characterizing catalytic domains in enzymes, preferably plant enzymes and those enzymes within the carotene synthesis family and more specifically ε-cyclase enzymes regulating formation of ε,ε-carotene. The methods include reverse PCR and site-directed mutagenesis for generating chimera and truncations or site-directed mutations of enzymes, respectively. These chimera, truncations or site directed mutants of ε-cyclase enzymes are useful in the characterization of the sequence residues conferring catalytic domains for the enzymes, and more specifically, the identification of single residues regulating catalytic activity for enzymes that are important in plant growth and photosynthesis. Chimeric enzymes generated by the methods of the invention can also be used to create transgenci hosts which are augmentated in their expression of specific carotene products.
专利号:CN-114196560-A 优先权日:2021-12-21 标题 :Genetic engineering bacterium for multi-stage and multi-level regulation and control of heterologous synthesis and construction method