专利号:WO-2023008461-A1 优先权日:2021-07-27 标题:Medicament for treatment and/or prevention of cancer 发明人:KUME MASAHIKO; OKANO FUMIYOSHI; SAITO TAKANORI 权利人:TORAY INDUSTRIES 摘要:The present invention relates to a medicament for the treatment and/or prevention of cancer, the medicament being characterized by comprising an antibody having an immunological reactivity with CAPRIN-1 protein or a fragment of the antibody and a drug capable of inhibiting the N-glycoside-linked sugar chain synthesis of a glycoprotein which are combined with each other in a blended or separated form.
专利号:US-9850473-B2 优先权日:2011-03-03 标 题:Transglycosylation activity of glycosynthase mutants of an endo-beta-N-acetylglucosaminidase (endo-D) from Streptococcus pneumoniae 发明人:WANG LAI-XI 权利人:UNIV MARYLAND 摘要:The present invention provides for recombinant Endo-D and selected mutants that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sugar chain is added to a core fucosylated or nonfucosylated GlcNAc-protein acceptor by transglycosylation. Such recombinant Endo-D and selected mutants are useful for efficient glycosylation remodeling of IgG1-Fc domain.
专利号:US-6271442-B1 优先权日:1990-12-20 标 题 :Method of producing pathogen-resistant plants 发明人:SCHELL JEFF; LOGEMANN JUERGEN; JACH GUIDO; MUNDY JOHN 权利人:MAX PLANCK GESELLSCHAFT 摘要:Described are a method of producing pathogen-resistant plants in which a protein-synthesis inhibitor gene or a fusion product of the protein-synthesis inhibitor gene or of the protein-synthesis inhibitor protein with ligands permitting specific attachment to cells is introduced into the genotype of plants under the control of an active promotor, and the use of the protein-synthesis inhibitor protein obtained by introducing the protein-synthesis inhibitor gene into the bacterial overproducers for making pharmaceutical preparations.
专利号:US-10851174-B2 优先权日:2011-03-03 标 题:Core fucosylated glycopeptides and glycoproteins: chemoenzymatic synthesis and uses thereof 发明人:WANG LAI-XI; HUANG WEI; GIDDENS JOHN 权利人:WANG Lai Xi; HUANG WEI; GIDDENS JOHN; UNIV MARYLAND 摘要:A chemoenzymatic method for the preparation of a core-fucoslyated glycoprotein or glycopeptide, including (a) providing an acceptor selected from the group consisting of a fucosylated GlcNAc-protein and fucosylated GlcNAc-peptide; and (b) reacting the acceptor with a donor substrate including an activated oligosaccharide moiety, in the presence of an endoglycosidase (ENGase) selected from Endo;F1, Endo-F2, Endo-F3, Endo-D and related glycosynthase mutants to transfer the oligosaccharide moiety to the acceptor and yield the structure defined core-fucosylated glycoprotein or glycopeptide. The donor substrate includes, in a specific implementation, a synthetic oligosaccharide oxazoline. A related method of fucosylated glycoprotein or fucosylated glycopeptide remodeling with a predetermined natural N-glycan or a tailor-made oligosaccharide moiety, and a method of remodeling an antibody to include a predetermined sugar chain to replace a heterogeneous sugar chain, are also described.
专利号:US-2024287564-A1 优先权日:2021-06-07 标题 :Acholetin biopolymers and methods for enzymatic synthesis 发明人:WITHERS STEPHEN G; MACDONALD SPENCER S S 权利人:UNIV BRITISH COLUMBIA 摘要:Provided herein are β-1,3-linked biopolymers (acholetin polysaccharides). Furthermore, provided herein are enzymatic methods and systems for producing β-1,3-linked oligosaccharides and polysaccharides using β-1,3-N-acetylglucosaminide phosphorylase (Acholetin phosphorylase (AchP)). The AchP was sourced from the genome of the cell wall-less Mollicute bacterium, Acholeplasma laidlawii and was found to synthesize β-1,3-linked N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc) oligomers using the donor. α-N-acetylglucosamine 1-phosphate (GlcNAc1-P) or N-acetylgalactosamine 1-phosphate (GalNAc1-P).
专利号:US-2023011163-A1 优先权日:2020-05-09 标题:Synthesis of human milk oligosaccharides by a Beta-N-acetylhexosaminidase from Haloferula sp.
参考文献:10.1080/09168451.2020.1805584 摘要:Ohno M, Miyazaki M, Kimura M, Minowa Y, Sakaguchi M, Oyama F, Yamashita T. Characterization of mouse di-N-acetylchitobiase that can degrade chitin-oligosaccharides. Biosci Biotechnol Biochem. 2020 Dec;84(12):2499–507. doi: 10.1080/09168451.2020.1805584. 参考文献:10.1074/jbc.m310614200 摘要:Hiruma T, Togayachi A, Okamura K, Sato T, Kikuchi N, Kwon YD, Nakamura A, Fujimura K, Gotoh M, Tachibana K, Ishizuka Y, Noce T, Nakanishi H, Narimatsu H. A novel human beta1,3-N-acetylgalactosaminyltransferase that synthesizes a unique carbohydrate structure, GalNAcbeta1-3GlcNAc. J Biol Chem. 2004 Apr 02;279(14):14087–95. doi: 10.1074/jbc.m310614200. 参考文献:10.1007/s00438-002-0640-2 摘要:Xiao X, Wang F, Saito A, Majka J, Schlösser A, Schrempf H. The novel Streptomyces olivaceoviridis ABC transporter Ngc mediates uptake of N-acetylglucosamine and N,N'-diacetylchitobiose. Molecular Genetics and Genomics. 2002 Apr 09;267(4):429–39. doi: 10.1007/s00438-002-0640-2.