CAS: 35599-02-1; (2R,3S,4S,5S,6S)-2-(Hydroxymethyl)-6-(4-Nitrophenoxy)Tetrahydro-2H-Pyran-3,4,5-Triol

该化合物是一种合成甘蓝衍生物,广泛用作色素基质,用于检测和检测β-mannosidase活动.化合物在酶水解过程中释放出黄色的p-nicontenalol,在405纳米时允许光谱光谱测量量化.其高纯度和稳定性使其适合用于酶学,遗传生物学和诊断学的研究应用.基质对β-mannosidas的特性确保了酶动力学和抑制研究定性的可靠结果.此外,其水溶解性便于直接准备实验性解决办法.该产品通常用于生物化学和临床研究,以研究碳水化合物活性酶和涉及含甘蔗的代谢物的代谢途径.

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合成工艺路线路线简述

    📜4-Nitrophenol Sodium Salt置于吡啶,磷酸酐,Calcium Sulfate,Sodium Tetrahydroborate,Sodium Methylate,Silver Carbonate体系中,用 甲醇,丙酮 作为反应溶剂,化学反应 21.5H,反应生成 4-硝基苯基-Beta-D-吡喃甘露糖苷
    参考文献:Synthesis Of 4-Nitrophenyl 2,3,6-Tri-O-Acetyl-4-Deoxy-β-D-Lyxo-Hexopyranoside And A New Synthesis Of 4-Nitrophenyl β-D-Mannopyranoside
    标题:Synthesis Of 4-Nitrophenyl 2,3,6-Tri-O-Acetyl-4-Deoxy-β-D-Lyxo-Hexopyranoside And A New Synthesis Of 4-Nitrophenyl β-D-Mannopyranoside
    摘要:
    DOI:10.1016/0008-6215(83)84067-1

    海关参考信息

    专利信息


    专利号:WO-2019234295-A1
    优先权日:2018-06-05
    标 题 :Beta glucosidase with high glucose tolerance, high thermal stability and broad ph activity spectrum
    发明人:MARJAMAA KAISA; KOIVULA ANU; ARO NINA; PAKULA TIINA
    权利人:TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
    摘要:The present invention relates to polypeptide having beta-glucosidase activity. Said activity is retained also in high glucose concentration. The invention also relates to an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing said polypeptide. Further the invention relates to compositions comprising said polypeptide and method of using the polypeptide in hydrolysis or synthesis.

    专利号:US-11371032-B2
    优先权日:2018-06-05
    标题 :Beta glucosidase with high glucose tolerance, high thermal stability and broad PH activity spectrum
    发明人:MARJAMAA KAISA; KOIVULA ANU; ARO NINA; PAKULA TIINA
    权利人:TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
    摘要:The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.

    供应商参考报价(招募中)

    品牌试剂参考报价(招募中)

    📌 第三方产品分析报告

    ✅ COA系统入驻 | 共享模式

    合成参考文献


    参考文献:10.1038/nchembio.81|10.2210/pdb2vjx/pdb|10.2210/pdb2vl4/pdb|10.2210/pdb2vmf/pdb|10.2210/pdb2vo5/pdb|10.2210/pdb2vot/pdb|10.2210/pdb2vqt/pdb|10.2210/pdb2vqu/pdb
    摘要:Tailford LE, Offen WA, Smith NL, Dumon C, Morland C, Gratien J, Heck MP, Stick RV, Blériot Y, Vasella A, Gilbert HJ, Davies GJ. Structural and biochemical evidence for a boat-like transition state in beta-mannosidases. Nat Chem Biol. 2008 May;4(5):306–12. doi: 10.1038/nchembio.81.
    参考文献:10.1016/j.nbt.2011.05.002
    摘要:Park SH, Park KH, Oh BC, Alli I, Lee BH. Expression and characterization of an extremely thermostable β-glycosidase (mannosidase) from the hyperthermophilic archaeon Pyrococcus furiosus DSM3638. N Biotechnol. 2011 Oct;28(6):639–48. doi: 10.1016/j.nbt.2011.05.002.
    参考文献:10.1007/s00253-012-4656-1
    摘要:Lu H, Zhang H, Shi P, Luo H, Wang Y, Yang P, Yao B. A family 5 β-mannanase from the thermophilic fungus Thielavia arenaria XZ7 with typical thermophilic enzyme features. Applied Microbiology and Biotechnology. 2013 Jan 09;97(18):8121–8. doi: 10.1007/s00253-012-4656-1.
    参考文献:10.1007/s10295-014-1459-7
    摘要:Malherbe AR, Rose SH, Viljoen-Bloom M, van Zyl WH. Expression and evaluation of enzymes required for the hydrolysis of galactomannan. Journal of Industrial Microbiology & Biotechnology. 2014 Jun 03;41(8):1201–9. doi: 10.1007/s10295-014-1459-7.
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