专利号:US-2007004010-A1 优先权日:2002-09-12 标题 :Engineered Enzymes and Their Use for Synthesis of Thioglycosides 发明人:WITHERS STEPHEN G; JAHN MICHAEL 权利人:UNIV BRITISH COLUMBIA 摘要:Mutant glycosidases in which the amino acid in the active site that serves as the acid, base or acid/base-catalyst is converted from a carboxylic acid to some other amino acid (for example to a simple alkyl, as in alanine or glycine) can catalyze the reaction of a thiosugar acceptor and an activated donor to form a thioglycoside. The “thioglycoligasesâ€? represent a novel class of mutant enzymes, and represent a first aspect of the invention. Thioglycoligases can be used in accordance with the method of the invention to couple a thiosugar acceptor and an activated donor to form a thioglycoside. By selection of the donor and acceptor species, as well as the specific enzyme employed, thioglycosides of different structure and stereochemistry can be obtained.
专利号:US-2006035342-A1 优先权日:2002-09-12 标 题:Engineered enzymes and their use for synthesis of thioglycosides
参考标题:Synthesis Of Novel Di- And Trisaccharide Mimetics With Non-Glycosidic Amino Bridges 作者:Janna Neumann,Saskia Weingarten,Joachim Thiem |发布日期:2007.3 摘要:Synthesis Of Novel Di- And Trisaccharides Using Enzymatic Glycosylation, Dess-Martin Oxidation And Reductive Amination Allows Rapid Access To The Target Structures. Thus, A Novel Class Of Glycomimetics Was Obtained Having Nitrogen Inserted As Bridging Atom Between Two Non-Anomeric Positions. Novel Di- And Trisaccharide Mimetics Were Designed Using N-Acetylglucosamine As A Basis Structure. A Third Monosaccharide
合成参考文献
参考文献:10.1007/s00253-020-10806-6 摘要:Velasco J, Oliva B, Gonçalves AL, Lima AS, Ferreira G, França BA, Mulinari EJ, Gonçalves TA, Squina FM, Kadowaki MAS, Maiorano A, Polikarpov I, Oliveira LCD, Segato F. Functional characterization of a novel thermophilic exo-arabinanase from Thermothielavioides terrestris. Applied Microbiology and Biotechnology. 2020 Aug 19;104(19):8309–26. doi: 10.1007/s00253-020-10806-6.