专利号:US-9458471-B2 优先权日:2006-11-03 标题 :Engineered cellular pathways for programmed autoregulation of differentiation 发明人:WEISS RON; LEMISCHKA IHOR; PURNICK PRISCILLA; SCHANIEL CHRISTOPH; MILLER MILES; GUYE PATRICK 权利人:UNIV PRINCETON 摘要:The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.
专利号:WO-2024256370-A1 优先权日:2023-06-13 标 题 :Synthesis of glufosinate using an amidase-based process 发明人:ZIMMERMANN GUNTHER; POTT MORITZ STEFAN 权利人:BASF SE 摘要:The present invention relates to a method of preparing glufosinate, comprising the steps of hydrolysing an N-carbamoyl glufosinate amide with an Amidase enzyme to form an N- carbamoyl amino acid compound followed by cleaving off the carbamoyl moiety of said N- carbamoyl amino acid compound.
专利号:US-2022145335-A1 优先权日:2022-01-27 标 题 :Bio-catalyzed Synthesis of Potent Anti-inflammatory Agents from Medroxyprogesterone Acetate 发明人:CHOUDHARY MUHAMMAD IQBAL; BANO SAIRA; WAHAB ATIA-TUL; UL-HAQ ZAHEER; YOUSUF SAMMER; JABEEN ALMAS; RAHMAN ATTA-UR 权利人:CHOUDHARY MUHAMMAD IQBAL; BANO SAIRA; WAHAB ATIA TUL; UL HAQ ZAHEER; YOUSUF SAMMER; JABEEN ALMAS; RAHMAN ATTA UR 摘要:Biotransformation of medroxyprogesterone acetate (MPA) (1) with Cunninghamella blakesleeana (ATCC 8688) yielded five new analogues, i.e. 17α-acetoxy-6α-methylpregn-4-ene-3,11,20-trione (2), 17α-acetoxy-15β-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (3), 17α-acetoxy-6β-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (4), 17α-acetoxy-11β,15β-dihydroxy-6α-methylpregn-4-ene-3,20-dione (5), and 17α-acetoxy-6β,11β-dihydroxy-6α-methylpregn-4-ene-3,20-dione (6). In T-cell proliferation assay, metabolites 2, and 5 were found to be potent inhibitors with IC50<0.5 μM, metabolite 6 showed a significant activity with IC50=8.64±0.02 μM, while metabolites 3 and 4 were found to be moderately active with IC50=41.59±8.14, and 40.14±0.12 μM, as compared to substrate 1 (IC50=6.48±5.18 μM) and standard prednisolone (IC50=9.75±0.03 μM) in in vitro assay. To establish the binding mode of medroxyprogesterone acetate (MPA) and the bio-transformed derivatives, molecular docking simulations were carried out using Vina.
专利号:US-2025120400-A1 优先权日:2021-12-10 标题:Synthesis of glufosinate using a hydantoinase-based process 发明人:DITRICH KLAUS; BREUER MICHAEL; POTT MORITZ STEFAN; ZIMMERMANN GUNTHER; SEEMAYER STEFAN 权利人:BASF SE 摘要:The present invention relates to a method of manufacturing glufosinate, comprising the steps of hydrolysing a hydantoin with a Hydantoinase enzyme to form a N-carbamoyl amino acid compound followed by cleaving off the carbamoyl moiety of said N-carbamoyl amino acid compound.
专利号:US-11919928-B2 优先权日:2016-03-16 标题 :Method for producing dihydrotentoxin 发明人:KUBO TAKASHI; MACHIDA MASAYUKI; FUJIOKA TOMONORI; YAMAGUCHI Shigenari; KAWAI KIYOSHI 权利人:KUMIAI CHEMICAL INDUSTRY CO 摘要:An object of the present invention is to identify an enzyme having activity of synthesizing dihydrotentoxin that is a tentoxin precursor and an enzyme having activity of synthesizing tentoxin using dihydrotentoxin as a substrate. The present invention concerns a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 16 and having activity of nonribosomal peptide synthesis of dihydrotentoxin and a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 and having activity of converting dihydrotentoxin to tentoxin.
专利号:US-2021386068-A1 优先权日:2018-11-07 标题 :Compositions comprising pyridine carboxylate herbicides and very long chain fatty acid (vlcfa) synthesis inhibitor herbicides 发明人:KISTER JEREMY; SATCHIVI NORBERT M 权利人:CORTEVA AGRISCIENCE LLC 摘要:Disclosed herein are compositions comprising (a) a pyridine carboxylate herbicide or an agriculturally acceptable N-oxide, salt, or ester thereof and (b) a VLCFA synthesis inhibitor herbicide. Also disclosed herein are methods of controlling undesirable vegetation, comprising applying to vegetation or an area adjacent the vegetation or applying in soil or water to control the emergence or growth of vegetation (a) a pyridine carboxylate herbicide or an agriculturally acceptable N-oxide, salt, or ester thereof, and (b) a VLCFA synthesis inhibitor herbicide.
1: Torabizadeh M, Tabar-Heydar K, Ahmadi SH. Online Solid-Phase Extraction of Prometon and Prometryne Using MIL-101(Cr) as Sorbent before Gas Chromatographic Analysis: A Computational and Experimental Study and Comparison between Splitless and PTV Inlets. J Chromatogr Sci. 2022 Nov 5;60(9):887-896. doi: 10.1093/chromsci/bmab140. 497-498:459-466. doi: 10.1016/j.scitotenv.2014.07.123. Epub 2014 Aug 20. 25(8):2143-53. doi: 10.1897/05-604r.1. 33(4):1260-6. Chinese. 322:138158. doi: 10.1016/j.chemosphere.2023.138158. Epub 2023 Feb 18. 52 Suppl 1:112-25. doi: 10.1111/gwat.12176. Epub 2014 Mar 5. 71(1):97-106. doi: 10.1016/j.chemosphere.2007.10.006. Epub 2007 Nov 26. 15(6):5483-5491. doi: 10.1007/s12274-022-4164-2. Epub 2022 Mar 15.
合成参考文献
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