CAS: 52571-71-8; (2R,3R,4S,5R,6R)-2-(Hydroxymethyl)-6-(3-Nitrophenoxy)Tetrahydro-2H-Pyran-3,4,5-Triol

该化合物是一种合成的晶质衍生物,通常用作酶性实验的铬诱导基质,特别是用于检测α-甲状腺异构体活动.该化合物的特点是与伽勒阳性表面运动有关的一个硝基苯组,该组在酶裂变后释放出黄色3-硝基甲酸盐,能够进行光谱光谱测量测量.在水温缓冲和稳定条件下,它具有较高的溶性,在标准的测量条件下,为研究和诊断应用提供了可靠的选择.硝化蛋白产品的独特吸收峰值(400纳米)允许进行精确和敏感的测量.该亚基体在微生物学,生物化学学和临床研究中广泛使用,以评价酶动力学或代谢紊乱的屏幕.

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D-galactose pentaacetate 2,3,4,6-tetra-O-acetyl-D-galactopyranosyl trichloroacetimidate Tetra-O-acetyl-m-nitrophenyl-α-D-galactopyranosid

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    专利信息


    专利号:US-5362625-A
    优先权日:1991-05-15
    标 题:Methods and compositions for enzyme complementation assays using the omega region of β-galactosidase
    发明人:KREVOLIN MARK; KATES DAVID
    权利人:MICROGENICS CORP
    摘要:The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the beta -galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypeptide synthesis techniques, which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase, is described along with improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample.

    专利号:US-5643734-A
    优先权日:1984-03-01
    标题 :Methods for protein binding enzyme complementation assays
    发明人:HENDERSON DANIEL ROBERT
    权利人:MICROGENICS CORP
    摘要:This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

    专利号:US-5120653-A
    优先权日:1985-04-08
    标 题:Vector comprising DNA sequence coding for enzyme-donor polypeptide
    发明人:HENDERSON DANIEL R
    权利人:MICROGENICS CORP
    摘要:This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

    专利号:EP-0199801-B1
    优先权日:1984-10-29
    标 题:Methods for protein binding enzyme complementation assays
    摘要:Improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristics of beta-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

    专利号:US-4708929-A
    优先权日:1984-10-29
    标 题:Methods for protein binding enzyme complementation assays
    发明人:HENDERSON DANIEL R
    权利人:MICROGENICS CORP
    摘要:This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of beta -galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

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    摘要:S55 | ZINC15PHARMA | Pharmaceuticals from ZINC15 | DOI:10.5281/zenodo.3247749
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