CAS: 696-04-8; 5-Methyldihydropyrimidine-2,4(1H,3H)-Dione

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CAS号65-71-4 胸腺嘧啶 | CAS号4433-40-3 5-羟甲基脲嘧啶 | CAS号80-62-6 甲基丙烯酸甲酯 | CAS号57-13-6 尿素 | CAS号79-41-4 甲基丙烯酸 | CAS号2905-86-4 3-(carbamoylami... | CAS号73-22-3 L-色氨酸 | CAS号1195-73-9 5-bromo-6-hydro... | CAS号1124-84-1 cis-thymineglycol | CAS号77928-77-9 2-methylbutaned... | CAS号65-71-4 胸腺嘧啶 | CAS号2417-22-3 5-甲基嘧啶-2,4,6(1H... | CAS号28100-77-8 5-thyminyl-5,6-... | CAS号2905-86-4 3-(carbamoylami... | CAS号22560-81-2 3-氨基-2-甲基丙酸乙酯 | CAS号10569-72-9 DL-3-氨基异丁酸水合物

胸腺嘧啶置于dihydropyrimidine Dehydrogenase From Escherichia Coli,还原型辅酶ⅰ体系中,化学反应生成 5,6-二氢-5-甲基尿嘧啶
参考文献:来自大肠杆菌的二氢嘧啶脱氢酶:瞬态分析揭示了相对于哺乳动物形式,转换前的还原激活和底物效应子作用的减弱
标题:来自大肠杆菌的二氢嘧啶脱氢酶:瞬态分析揭示了相对于哺乳动物形式,转换前的还原激活和底物效应子作用的减弱
摘要:二氢嘧啶脱氢酶 (Dpd) 是一种酶,它使用复杂的结构来催化简单的净反应:尿嘧啶和胸腺嘧啶的乙烯基键的还原.已知的 Dpd 具有两个相距约 60 å 的活性位点.一个活性位点具有 Fad 辅因子并结合 Nad(P),另一个活性位点具有 Fmn 辅因子并结合嘧啶.中间距离由四个充当电子导管的 Fes 中心跨越.猪 Dpd 的最新进展揭示了意想不到的化学序列,其中酶通过 Fad 将两个电子从 Nadph 转移到 Fmn 进行还原激活,从而活性形式具有辅因子组 Fad•4(Fes)•fmnh.在这里,我们描述了对细菌形式 Dpd 的首次全面动力学研究.主要使用瞬态方法,来自(ecdpd)的dpd被证明具有与在哺乳动物形式中观测到的相似机制,因为观测到ecdpd在嘧啶还原之前经历还原激活并显示出半位点活性.然而,观测到与哺乳动物酶相关的 Ecdpd 反应的两个不同方面,这些方面与底物的效应子作用有关:(I)
DOI:10.1016/j.Abb.2023.109772

专利信息

专利号:US-12421628-B2
优先权日:2018-07-23
标题:Massively parallel enzymatic synthesis of nucleic acid strands
发明人：HORGAN ADRIAN; GODRON XAVIER; YBERT THOMAS; NICOL ROBERT
权利人:DNA SCRIPT
摘要:The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.

专利号:US-11858953-B2
优先权日:2018-04-04
标 题:Compositions and methods for synthesis of phosphorylated molecules
发明人：CHAPUT JOHN; LIAO JEN-YU; BALA SAIKAT
权利人:UNIV CALIFORNIA
摘要:The invention provides compositions and methods for synthesis of phosphorylated organic compounds, including nucleoside triphosphates.

专利号:US-2024052391-A1
优先权日:2020-10-29
标 题 :Enzymatic Synthesis of Polynucleotide Probes
发明人：GODRON XAVIER; NIYOMCHON SUPAPORN; CHAMPION ELISE
权利人:DNA SCRIPT
摘要:The present invention is directed to methods and kits for enzymatic synthesis of labeled polynucleotide probes using template-free DNA polymerases.

专利号:WO-2024073349-A9
优先权日:2022-09-26
标 题:Cleavable linkers for the tethering of polymerases to nucleotides
发明人：FELDMAN AARON; PALLUK SEBASTIAN; ARLOW DANIEL; BERTRAM JEFFREY GEORGE; RALISKI BEN; RAINES RONALD T; BORNSCHEUER UWE THEO; FESSNER NICO DENNIS; BADENHORST CHRISTOFFEL PETRUS STEPHANUS
权利人:ANSA BIOTECHNOLOGIES INC
摘要:The present disclosure provides methods of polynucleotide synthesis and compounds, compositions useful for synthesis of polynucleotides. The chemical compounds include nucleotides and their analogs that are attached to polymerase though a cleavable linker comprising an enzymatically cleavable amino acid ester.

专利号:US-9751909-B2
优先权日:2011-09-07
标 题 :Synthesis and uses of nucleic acid compounds with conformationally restricted monomers
发明人：MATRAY TRACY J; MACIAGIEWICZ IWONA M; HOUSTON JR MICHAEL E
权利人:MATRAY TRACY J; MACIAGIEWICZ IWONA M; HOUSTON JR MICHAEL E; MARINA BIOTECH INC
摘要:Processes for synthesis of conformationally restricted nucleomonomers (CRN) by ozonolysis or oxidation of a 2′-vinyl substituted nucleoside precursor with protected 3′-hydroxyl, 4′-hydroxymethyl and 5′-hydroxyl positions. The CRN nucleomonomers can be used to prepare nucleic acid compounds. CRN nucleomonomers for nucleic acid compounds can be prepared in high yields and in multi-gram scale. The nucleic acid compounds can be of various regulatory classes such as antisense RNA for therapeutic modalities useful for treating or preventing diseases or disorders by up- or down-regulating the expression of genes and other nucleic acid based regulatory systems in a cell.

专利号:EP-1419247-B1
优先权日:2001-07-31
标 题 :Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
发明人：KOLTERMANN ANDRE; KETTLING ULRICH; GREINER-STOEFFELE THOMAS; SPANGENBERG OLIVER
权利人:DIREVO BIOTECH AG
摘要:Methods and kit for producing recombinant chimeric polynucleotides comprising: forming heteroduplices with single-stranded, partially heterologous source nucleic acids which contain at least one marker nucleotide, introducing single-stranded nicks at sites of the marker nucleotides, and starting synthesis of single-stranded nucleic acids at the nick sites, the non-nicked strand serving as template.