CAS: 6920-38-3; 5,7-Dihydroxy-2-(3-Hydroxy-4-(((2S,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Oxy)Phenyl)-4H-Chromen-4-One

上下游产品

luteolin 4'-O-β-D-glucopyranoside 7-O-β-D-glucopyranoside UDP-glucose 2-(3,4-Dihydroxy-phenyl)-5,7-dihydroxy-chromen-4-on

📜尿苷(5')二氢二磷酰(1)-Alpha-D-葡萄糖,木犀草素置于populus Deltoids Marsh Pgt-3体系中,化学反应 0.5H,反应生成 藤黄菌素-4'-O-葡糖苷
参考文献:Biological Synthesis Of Isorhamnetin 3-O-Glucoside Using Engineered Glucosyltransferase
标题:Biological Synthesis Of Isorhamnetin 3-O-Glucoside Using Engineered Glucosyltransferase
摘要:The Gene For One Of The Glycosyltransferases From Populus Deltoids,Pgt-3,Was Cloned And Was Expressed As A Glutathione S-Transferase Fusion Protein In Escherichia Coli. Various Flavonoids Were Used As Potential Substrates Of The Purified Recombinant Pgt-3. Flavones Having Two Adjacent Hydroxyl Groups Were Served As Substrate. The Regioselectivity Of Pgt-3 Depends On The Hydroxyl Groups Of The Substrate. Flavones Having Two Adjacent Hydroxyl Groups In The B Ring Were Glucosylated At The 4'-Hydroxyl Group. However. Pgt-3 Transferred A Glucose Group To The 3-Hydroxyl Group Of Isorhamnetin. Molecular Modeling And Docking And Site-Directed Mutagenesis Were Carried Out To Engineer A Pgt-3 Having A Specificity For Isorhamnetin But Not For Quercetin. Glu82Leu Turned Out To Display This Activity. Using The Glu82Leu Mutant And A Quercetin 3'-O-Methyltransferase,Isorhamnetin 3-O-Glucoside Was Synthesized. (C) 2010 Elsevier B.V. All Rights Reserved.
DOI:10.1016/j.Molcatb.2010.01.012

专利信息

专利号:CN-117431285-B
优先权日:2023-10-20
标 题:Application of corn glycosyltransferase ZmUGT84A1 and ZmUGT84A2 in the synthesis of flavonoid glycoside derivatives

专利号:CN-117431285-A
优先权日:2023-10-20
标题:Application of corn glycosyltransferase ZmUGT84A1 and ZmUGT84A2 in synthesis of flavone glycoside derivatives