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📜二碳酸二叔丁酯,Dl-7-氮杂色氨酸置于三乙胺体系中,用 1,4-二氧六环,水 用作溶剂,化学反应 5.5H,以72%的收率获得boc-Dl-7-氮杂色氨酸
参考文献:In Vitro Site-Specific Incorporation Of Fluorescent Probes Into β-Galactosidase
标题:In Vitro Site-Specific Incorporation Of Fluorescent Probes Into β-Galactosidase
摘要:Fluorescence Spectroscopy Is A Powerful Biophysical Technique For Studying Protein Structure,Function,Dynamics,And Intermolecular Interactions. Such Studies Are Often Conducted Using Intrinsic Probes,Such As Tryptophan Residues,Or Extrinsic Probes Introduced By Post-Translational Modification,Such As Dansyl. Specificity,However,Is Often A Concern Since Many Proteins Contain More Than One Tryptophan And Chemical Modification Often Will occur At More Than One Site. Herein We Report The In Vitro,Site-Specific Incorporation Of Three Fluorescent Amino Acid Analogues,5-Hydroxytryptophan,7-Azatryptophan,And Epsilon-Dansyllysine,Each Of Which Was Incorporated Into Beta-Galactosidase At A Single Designated Site.
Doi:10.1021/ja963023F