CAS: 21414-41-5; (((5-(Methylsulfinyl)-1-(((2S,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Thio)Pentylidene)Amino)Oxy)Sulfonic Acid

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CAS号4478-93-7 萝卜硫素

芝麻菜苷置于双氧水体系中,用 甲醇 用作溶剂,以100%的收率获得萝卜硫苷
参考文献:Cytotoxic And Antioxidant Activity Of 4-Methylthio-3-Butenyl Isothiocyanate From Raphanus Sativus L. (Kaiware Daikon) Sprouts
标题:Cytotoxic And Antioxidant Activity Of 4-Methylthio-3-Butenyl Isothiocyanate From Raphanus Sativus L. (Kaiware Daikon) Sprouts
摘要:There Is High Current Interest In The Chemopreventive Potential Of Brassica Vegetables (Cruciferae),Particularly Due To Their Content In Glucosinolates (Gl),Which Upon Myrosinase Hydrolysis,Release The Corresponding Isythiocyanates (Itc). Some Itcs,Such As Sulforaphane (Sfn) From Broccoli (Brassica Oleacea Italica),Have Been Found To Possess Anticancer Activity Through Induction Of Apoptosis In Selected Cell Lines,As Well As Indirect Antioxidant Activity Through Induction Of Phase 11 Detoxifying Enzymes. Japanese Daikon (Raphanus Sativus L.) Is Possibly The Vegetable With The Highest Per Capita Consumption Within The Brassicaceae Family. Thanks To A Recently Improved Gram Scale Production Process,It Was Possible To Prepare Sufficient Amounts Of The Gl Glucoraphasatin (Grh) As Well As The Corresponding Itc 4-Methylthio-3-Butenyl Isothiocyanate (Grh-Itc) From Its Sprouts. This Paper Reports A Study On The Cytotoxic And Apoptotic Activities Of Grh-Itc Compared With The Oxidized Counterpart 4-Methylsulfinyl-3-Butenyl Isothiocyanate (Gre-Itc) On Three Human Colon Carcinoma Cell Lines (Lovo,Hct-116,And Ht-29) Together With A Detailed Kinetic Investigation Of The Direct Antioxidant/radical Scavenging Ability Of Grh And Grh-Itc. Both Grh-Itc And Gre-Itc Reduced Cell Proliferation In A Dose-Dependent Manner And Induced Apoptosis In The Three Cancer Cell Lines. The Compounds Significantly (P < 0 05) Increased Bax And Decreased Bcl2 Protein Expression,As Well As Producing Caspase-9 And Parp-1 Cleavage After 3 Days Of Exposure In The Three Cancer Cell Lines. Grh-Itc Treatment Was Shown To Have No Toxicity With Regard To Normal Human Lymphocytes (-15 +/-5%) In Comparison With Sfn (Complete Growth Inhibition). Grh And Grh-Itc Were Able To Quench The 2,2-Diphenyl-1-Picrylhydrazyl Radical,With Second-Order Rate Constants Of 14.0 +/-2.8 And 43.1 +/-9.5 M-1 S(-1),Respectively (At 298 K In Methanol),Whereas The Corresponding Value Measured Here For The Reference Antioxidant Alpha-Tocopherol Was 425 +/-40 M-1 S(-1). Grh Reacted With H2O2 And Tert-Butyl Hydroperoxide In Water (Ph 7.4) At 37 Degrees C,With Rate Constants Of 1.9 +/-0.3 X 10(-2) And 9.5 +/-0.3 X 10(-4) M-1 S(-1) (Paralleling Recently Developed Synthetic Antioxidants) Being Quantitatively (>97%) Converted To Gre. It Is Demonstrated That Grh-Itc Has Interesting Antioxidant/radical Scavenging Properties,Associated With A Selective Cylotoxic/apoptotic Activity Toward Three Human Colon Carcinoma Cell Lines,And Very Limited Toxicity On Normal Human T-Lymphocytes.
Doi:10.1021/jf073123C

专利信息

专利号:US-7250559-B2
优先权日:2001-07-05
标 题:Nucleic acids encoding glucosinolate biosynthesis enzymes and methods of their use
发明人：QUIROS CARLOS; LI GENYI
权利人:UNIV CALIFORNIA
摘要:Provided are nucleic acid sequences from Brassica oleracea plants that encode enzymes involved in the synthesis of a glucosinolate molecule, including BoGSL-ALK, and BoGSL-ELONG, and methods of their use. The sequences find particular use in modifying the glucosinolate content of a plant. Also provided are primers for these two genes and a third key gene in the glucosinolate pathway, BoGSL-PRO, that can be used for developing molecular markers for assisted selection of plants with specific glucosinolate compositions. Additionally, compositions and methods for a simple, reliable and efficient PCR-based marker system, named sequence-related amplification polymorphism (SRAP), that finds use in the identification of coding sequences in the genome of a plant are provided.

专利号:CN-120209105-A
优先权日:2025-03-18
标 题 :Application of BoHY gene or coded protein thereof in promoting synthesis of broccoli radish thioglycoside

专利号:US-2021317497-A1
优先权日:2018-06-22
标题:Monbretin a (mba) synthesis using a heterologous nucleic acid(s) encoding a mba pathway enzyme
发明人：BOHLMANN CARL JOERG; IRMISCH SANDRA; JO SEOHYUN
权利人:UNIV BRITISH COLUMBIA
摘要:Provided are transgenic organisms, such as plants and plant parts, cells, and related compositions and methods for producing and monitoring the genotype for enhanced production of montbretin A and/or its precursors. For example, provided is a transgenic organism comprising at least one heterologous nucleic acid operatively linked to a promoter, wherein the heterologous nucleic acid encodes at least one enzyme in a montbretin A (MbA) metabolic pathway. The organisms can be a plant, plant part, or plant cell, or a microorganism such as a yeast. Also provided is a method for producing at least one montbretin A (MbA) precursor and/or MbA, comprising permitting the expression of the at least one heterologous nucleic acid in the transgenic organism. The disclosure also provides isolated nucleic acid molecules that comprise sequence encoding at least one enzyme in a montbretin A (MbA) metabolic pathway and vectors comprising the nucleic acids.

专利号:CN-117551673-A
优先权日:2023-11-10
标 题:Application of glycosyltransferase RrUGT3 gene in the synthesis of N-glycosides and S-glycosides

专利号:CN-111034616-A
优先权日:2019-12-25
标题 :A method for the synthesis and release of glucoraphanin and sulforaphane in hairy roots of broccoli

专利号:CN-111034616-B
优先权日:2019-12-25
标 题:A method for the synthesis and release of glucoraphanin and sulforaphane in hairy roots of broccoli