专利号:EP-1167524-B1 优先权日:1999-03-19 标题 :Method for amplifying a nucleic acid sequence employing a chimeric primer 发明人:MUKAI HIROYUKI; YAMAMOTO JUNKO; TAKEDA OSAMU; MIYAKE KAZUE; UEMORI TAKASHI; SATO YOSHIMI; MORIYAMA MARIKO; SAWARAGI HARUHISA; HAGIYA MICHIO; ASADA KIYOZO; KATO IKUNOSHIN 权利人:TAKARA BIO INC 摘要:A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
专利号:US-6951722-B2 优先权日:1999-03-19 标题:Method for amplifying nucleic acid sequence 发明人:MUKAI HIROYUKI; SAGAWA HIROAKI; UEMORI TAKASHI; YAMAMOTO JUNKO; TOMONO JUN; KOBAYASHI EIJI; ENOKI TATSUJI; TAKEDA OSAMU; MIYAKE KAZUE; SATO YOSHIMI; MORIYAMA MARIKO; SAWARAGI HARUHISA; HAGIYA MICHIO; ASADA KIYOZO; KATO IKUNOSHIN 权利人:TAKARA BIO INC 摘要:A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
[参考文献]: G Galaverna, Et Al. Diaminomethane Dihydrochloride, A Novel Reagent For The Synthesis Of Primary Amides Of Amino Acids And Peptides From Active Esters. Int J Pept Protein Res. 1993 Jul;42(1):53-7.
合成参考文献
摘要:National Toxicology Program, Institute of Environmental Health Sciences, National Institutes of Health (NTP). 1992. National Toxicology Program Chemical Repository Database. Research Triangle Park, North Carolina.