CAS: 130304-80-2; Fmoc-Asp(Ochex)-Oh

相似化合物

73821-95-1 150047-85-1 1799418-06-6

📜在 Sodium Thiosulfate,Magnesium Iodide体系中,用 2-甲基四氢呋喃 用作溶剂,化学反应 1.0H,以97%的收率获得芴甲氧羰基-天冬氨酸-4环己脂
参考文献:Mgi2-化学选择性切割可去除氨基酸保护基:肽合成的新视野
标题:Mgi2-化学选择性切割可去除氨基酸保护基:肽合成的新视野
摘要:在肽合成领域,成功接近合成靶标的关键在于保护基团的相关组合.他们的选择取决于他们的选择性去除条件.我们在这里介绍了肽化学中一些最常用的保护基团在实验裂解条件下的行为,结合了mgi 2和mw辐射,并使用2-Me-thf作为绿色溶剂.在这些实验条件下,可以在肽化学中重新考虑苄氧羰基保护基以及merrifield树脂.
Doi:10.1002/bip.22908

专利信息

专利号:US-10253061-B2
优先权日:2014-10-10
标 题:Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
发明人：QUAEDFLIEG PETER JAN LEONARD MARIO; NUIJENS TIMO; VAN DER LAAN JAN METSKE; JANSSEN DIRK BAREND; TOPLAK ANA; WU BIAN
权利人:ENZYPEP B V
摘要:The invention relates to a method for enzymatically synthesizing an (oligo)peptide, comprising coupling (a) an (oligo)peptide C-terminal ester or thioester and (b) an (oligo)peptide nucleophile having an N-terminally unprotected amine,n wherein the coupling is carried out in a fluid comprising water, and wherein the coupling is catalyzed by a subtilisin BPN′ variant or a homolog thereof, which comprises the following mutations compared to subtilisin BPN′ represented by SEQUENCE ID NO: 2 or a homolog sequence thereof:n a deletion of the amino acids corresponding to positions 75-83; a mutation at the amino acid position corresponding to S221, the mutation being S221C or S221 selenocysteine; n preferably a mutation at the amino acid position corresponding to P225 wherein the amino acid positions are defined according to the sequence of subtilisin BPN′ represented by SEQUENCE ID NO: 2. nn Further, the invention relates to an enzyme suitable for use as a catalyst in a method of the invention.