CAS: 69254-37-1; 5,6,8,9,11,12,14,15-Octadeuterioicosa-5,8,11,14-Tetraenoic Acid

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82-arachidonoyl glycerol-d8

📜2-Arachidonoyl Glycerol-D8置于mouse Brain Extracts Containing Membrane Bound Monoacrylglycerol Lipase (Magl)体系中,化学反应 0.5H,反应生成 1-Arachidonoylglycerol-D8,氘代花生四烯酸-5,6,8,9,11,12,14,15-D8
参考文献:Simultaneous Determination Of 2-Arachidonoylglycerol,1-Arachidonoylglycerol And Arachidonic Acid In Mouse Brain Tissue Using Liquid Chromatography/tandem Mass Spectrometry
标题:Simultaneous Determination Of 2-Arachidonoylglycerol,1-Arachidonoylglycerol And Arachidonic Acid In Mouse Brain Tissue Using Liquid Chromatography/tandem Mass Spectrometry
摘要:内源性大麻素(ecs),如阿奈酰胺(aea)和2-花生四烯酸甘油酯(2-Ag),调节多种生理过程,包括疼痛,食欲和情绪状态.ecs的水平在体内通过酶的生物合成和降解被严格控制.然而,关于终止主要脑内ec(2-Ag)信号传导的酶的知识仍然有限.鉴定和定量2-Ag,1-Ag和花生四烯酸(aa)对于研究2-Ag的酶水解非常重要.我们开发了一种灵敏且特异的定量方法,通过液相色谱/串联质谱(lc/ms/ms)利用简单的脑样本制备方法,能够同时测定小鼠脑和脂肪组织中的2-Ag,1-Ag和aa.分离是基于反相色谱进行的.电喷雾离子化条件的优化确定了检测限(信噪比=3),2-Ag,1-Ag和aa的检测限分别为50,25和65 Fmol.这些方法在2-Ag,1-Ag和aa的范围内具有选择性,精确性(相对标准偏差<10%)和灵敏性,分别为0.02-20,0.01-10和0.05-50 Ng/mg组织.定量方法经过考虑基质效应和分析物及相对氘标记内部标准(is)的质谱响应进行了验证.这些方法应用于研究小鼠脑提取物中含膜结合单酰基甘油脂肪酶(magl)的2-Ag水解,并测定小鼠脑和脂肪组织中2-Ag,1-Ag和aa的基础水平.版权 © 2009 John Wiley & Sons,Ltd.
DOI:10.1002/jms.1701

专利信息

专利号:US-2010303887-A1
优先权日:2007-07-30
标题:DHA and PEDF, a Therapeutic Composition for Nerve and Retinal Pigment Epithelial Cells
发明人：BAZAN NICOLAS G
权利人:BAZAN NICOLAS G
摘要:The combination of pigment epithelium-derived factor (PEDF) and docosahexaenoic acid (DHA) has been discovered to act synergistically to enhance cell survival and decrease apoptosis in retinal pigment epithelial (RPE) cells. PEDF and DHA synergistically protected RPE cells by confronted with oxidative stress by blocking apoptotic cell death and increasing the synthesis of the important mediator neuroprotectin D 1. Administering a composition comprising PEDF and DHA will halt or slow down the initiation and progression of macular degeneration, retinitis pigmentosa and retinal degeneration. In addition, the topical application of the combination of PEDF and DHA was found to promote cornea nerve regeneration after refractive surgery, and thus this combination could be used to prevent the complications of refractive surgery and certain diseases, e.g., neurotrophic keratitis due to Herpes virus.