CAS: 578-76-7; 2-Amino-7-Methyl-1H-Purin-6(7H)-One

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CAS号22164-16-5 Guanosine, 7-me... | CAS号66224-66-6 6H-Purin-6-imin... | CAS号81100-62-1 7-methylguanosi... | CAS号10310-21-1 2-氨基-6-氯嘌呤 | CAS号1198-47-6 2-氨基-6-巯嘌呤 | CAS号1074-89-1 6-甲氧基嘌呤 | CAS号2004-03-7 6-甲基嘌呤 | CAS号74-87-3 氯甲烷 | CAS号73-40-5 鸟嘌呤 | CAS号2181-42-2 三甲基碘代磺酸 | CAS号113-00-8 guanidine

📜2'-Deoxy-7-Methylguanosinium Iodide置于水体系中,化学反应 168.0H,反应生成 7-甲基鸟嘌呤
参考文献:使用核苷磷酸化酶制备嘌呤和嘧啶2'-脱氧核苷
标题:使用核苷磷酸化酶制备嘌呤和嘧啶2'-脱氧核苷
摘要:酶促糖基化反应(碳水化合物部分从一个杂环碱基到另一个杂环碱基的转移)正在积极开发中,并用于合成实际上重要的核苷.该反应由核苷磷酸化酶(nps)催化,核苷磷酸化酶负责核苷的可逆磷酸分解,以产生相应的杂环碱基和单糖1-磷酸.我们发现7-甲基-2'-脱氧鸟苷(7-Me-Dguo)是酶促糖基化反应中2-脱氧核糖部分的高效且新颖的供体,用于嘌呤和嘧啶2'-脱氧核糖核苷的合成,收率很高.与7-甲基鸟苷不同,其2'-脱氧衍生物的稳定性要差得多.幸运的是,我们发现7-甲基-2'-脱氧鸟苷氢碘酸盐可以在tris-Hcl缓冲液(ph 7)中保存24小时.5)在室温下无明显分解.为了优化试剂比例,在环境温度下进行了一系列分析性转糖基化反应.根据转糖基化反应的HPLC分析,使用少量过量(1.5和2.0当量)的五氯乙烷(1.5和2.0当量)可得到高产率(84-93%)的产物5-乙基-2'-脱氧尿苷(5-Et-Durd).
DOI:10.1002/adsc.201701005

专利信息

专利号:US-9920084-B2
优先权日:2011-08-23
标题 :Ionic tags for synthesis of oligoribonucleotides
发明人：DAMHA MASAD J; HASSLER MATTHEW; CHAN TAK-HANG; NANDYALA MALLIKARJUNA REDDY; DONGA ROBERT ALEXANDER
权利人:DAMHA MASAD J; HASSLER MATTHEW; CHAN TAK HANG; NANDYALA MALLIKARJUNA REDDY; DONGA ROBERT ALEXANDER; THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIV; HONG KONG POLYTECHNIC UNIV
摘要:The invention relates to the chemical synthesis of oligonucleotides, e.g., oligoribonucleotides. In another aspect, the invention relates to compounds of formula (II) processes for making these compounds, and the use thereof in the chemical synthesis of oligonucleotides, e.g., oligoribonucleotides. The invention also relates to methods of synthesis of oligomers, including but not limited to oligopeptides, oligosaccharides and oligonucleotides, particularly oligoribonucleotides and also oligodeoxyribonucleotides, in solution systems, and ionic tag linkers for use in methods provided herein.

专利号:US-12188072-B2
优先权日:2018-03-19
标题 :Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
发明人：JEWETT MICHAEL CHRISTOPHER; STARK JESSICA CAROL; DELISA MATTHEW P; JAROENTOMEECHAI THAPAKORN
权利人:UNIV NORTHWESTERN; UNIV CORNELL
摘要:Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated carrier proteins. The glycosylated carrier proteins may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated carrier proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. The glycosylated carrier proteins may be synthesized in cell-free glycoprotein synthesis (CFGpS) systems using prokaryote cell lysates that are enriched in components for glycoprotein synthesis such as oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs) including OSTs and LLOs associated with synthesis of bacterial O antigens.

专利号:US-12365930-B2
优先权日:2016-07-14
标题:Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
发明人：JEWETT MICHAEL CHRISTOPHER; STARK JESSICA CAROL; DELISA MATTHEW P; JAROENTOMEECHAI THAPAKORN
权利人:UNIV NORTHWESTERN; UNIV CORNELL
摘要:Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins. The glycosylated proteins may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. The glycosylated proteins may be synthesized in cell-free glycoprotein synthesis (CFGpS) systems using prokaryote cell lysates that are enriched in components for glycoprotein synthesis such as oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs) including OSTs and LLOs associated with synthesis of bacterial O antigens.

专利号:US-2024287520-A1
优先权日:2021-06-30
标题:Method for synthesis of linkage modified oligomeric compounds
发明人：RODRIGUEZ ANDREW A
权利人:IONIS PHARMACEUTICALS INC
摘要:The present disclosure provides methods of synthesizing modified oligonucletodies and oligomeric compounds (including oligomeric compounds that are antisense agents or portions thereof) comprising a modified oligonucleotide having at least one modified internucleoside linking group. In certain embodiments, the present disclosure provides stabilized formulations of certain sulfonyl azides for use in the synthesis of olignonucleotides comprising one or more sulfonyl phosphoramidate linkages. Some embodiments provide stabilized compositions of high energy reagents that may be used in the synthesis of modified oligonucleotides, allowing for safe process scale preparation thereof.

专利号:US-2004152905-A1
优先权日:2003-01-31
标题:Universal building blocks and support media for synthesis of oligonucleotides and their analogs
发明人：GUZAEV ANDREI P; MANOHARAN MUTHIAH
摘要:Compounds for the synthesis of oligomeric compounds, particularly oligonucleotides and chemically modified oligonucleotide analogs, are provided. In addition, methods for functionalization of a support medium with a first monomeric subunit and methods for the synthesis of oligomeric compounds utilizing the novel compounds bound to support media are provided.

专利号:US-2004242897-A1
优先权日:2002-07-31
标题 :Universal support media for synthesis of oligomeric compounds
发明人：GUZAEV ANDREI P; MANOHARAN MUTHIAH
摘要:Compounds for the synthesis of oligomeric compounds, particularly oligonucleotides and oligonucleotide mimetics, are provided. In addition, methods for functionalizing a support medium with a first monomeric subunit and methods for the synthesis of oligomeric compounds utilizing the novel compounds bound to support media are provided.