CAS: 3326-34-9; 5-Amino-3',6'-Dihydroxy-3H-Spiro[isobenzofuran-1,9'-Xanthen]-3-One

上下游产品

CAS号3326-35-0 4-硝基荧光素 | CAS号610-27-5 4-硝基邻苯二甲酸 | CAS号108-46-3 间苯二酚 | CAS号14926-29-5 5-硝基-二乙酸荧光素 | CAS号2307-00-8 4-氨基-N-甲基邻苯二甲酰亚胺 | CAS号3326-32-7 异硫氰酸荧光素酯 | CAS号209540-56-7 5-(PENTAFLUOROB... | CAS号75350-46-8 荧光素-5-马来酰亚胺 | CAS号2321-07-5 荧光素 | CAS号77162-08-4 azidofluorescein

📜硝基环己烷置于sodium Sulfide,Sodium Hydrogen Sulfate,Cobalt(II) Chloride,Zinc(II) Chloride体系中,用 水 作为反应溶剂,化学反应 14.0H,反应生成 5-氨基荧光素
参考文献:一种具有特异选择性的荧光素探针的制备方法
标题:一种具有特异选择性的荧光素探针的制备方法
摘要:本申请涉及具有特异选择性的荧光素探针的制备领域,具体公开了一种具有特异选择性的荧光素探针的制备方法.所述方法包括:(1)将4‑硝基邻苯二甲酸酐,间苯二酚,硝基环己烷和无水氯化钴混合,加热熔融,加入氯化锌,反应,之后加盐酸回流,过滤洗涤,干燥;(2)将包含na2S和na2So3的还原溶液与步骤(1)的所得物混合,反应,之后滴加冰醋酸至沉淀完全,过滤洗涤,干燥;(3)将包含甲醇和三乙胺的混合溶剂与步骤(2)的所得物混合,滴加cs2,反应,之后滴加氯化铜水溶液,滴完继续反应,之后用盐酸调节体系ph值为1~2,减压蒸馏,洗涤,干燥,柱色谱纯化.所述方法极大提高了5‑异硫氰酸荧光素的转化率和产率.

专利信息

专利号:EP-1749870-B1
优先权日:2005-08-04
标 题:Fluorescein-based compounds and their use for peptide synthesis
发明人：BALAKIREV MAXIM; BURCHAK OLGA
权利人:COMMISSARIAT ENERGIE ATOMIQUE
摘要:The present invention is related to new fluorescein derivatives, the method for producing such derivatives and their use for the synthesis of fluorogenic peptides and in particular protease substrates and peptide ligands.

专利号:US-6229055-B1
优先权日:1996-04-12
标题 :Synthesis of fluorinated xanthene derivatives
发明人：KLAUBERT DIETER H; GEE KYLE R
权利人:MOLECULAR PROBES INC
摘要:Facile syntheses for fluorinated resorcinol and aminophenol derivatives are provided that yield isomer-free products in good yield. These novel methods use generally available precursors and standard laboratory reagents and equipment to reproducibly produce these synthetically useful reagents in relatively large quantities. The resulting fluorinated resorcinols and aniinophenols possess utility in the preparation of fluorinated fluorescein and rhodol dyes.

专利号:WO-9518971-A1
优先权日:1994-01-11
标 题 :Methods for the solid phase synthesis of glycoconjugates
发明人：VETTER DIRK; TUMELTY DAVID A; ANTONENKO VALERY
权利人:AFFYMAX TECH NV; VETTER DIRK; TUMELTY DAVID A; ANTONENKO VALERY
摘要:An efficient and versatile method of forming N-linked glycoconjugates is described wherein a glycosyl acceptor, typically comprising an activated carboxyl group, is reacted with a glycosylating agent, typically a glycosylamine, in the presence of a coupling catalyst and optionally an exogenous base. Depending on the choice of reactive site, this method can be utilized to form N-linked glycoconjugates, in either a soluble or substrate-bound, linear or branched format.

专利号:US-5691456-A
优先权日:1992-03-30
标题 :Reagents and methods for the detection and quantification of thyroxine in fluid samples
发明人：ADAMCZYK MACIEJ; JOHNSON DONALD D; MATTINGLY PHILLIP G; CLARISSE DIANA E; TYNER JOAN D; PERKOWITZ MARY M
权利人:ABBOTT LAB
摘要:Immunoassay methods and reagents for the specific quantification of thyroxine in a test sample are disclosed employing antibodies prepared with thyroxine derivatives of the formula: wherein P is an immunogenic carrier material and X is a linking moiety. The present invention also describes the synthesis of unique labelled reagent of the formula: wherein Q is a detectable moiety and W is a linking moiety, preferably fluorescein or a fluorescein derivative.

专利号:US-5128419-A
优先权日:1990-08-20
标 题:Synthesis of tagged polymers by post-polymerization (trans) amidation reaction
发明人：FONG DODD W; HOOTS JOHN E
权利人:NALCO CHEMICAL CO
摘要:Polymers tagged with pendant fluorescent groups are prepared by the (trans)amidation derivatization of pre-existing polymers having carbonyl-type pendant groups. Polymers having pendant amide groups wherein the amide nitrogen is substituted with fluorescent moieties, prepared by (trans)amidation drrivatization, are provided. Polymers having pendant (sulfonated) napthalene moieties substituted to amide nitrogen are provided.

专利号:US-5260386-A
优先权日:1990-08-20
标 题 :Synthesis of tagged polymers by post-polymerization (trans) amidation reaction
发明人：FONG DODD W; HOOTS JOHN E
权利人:NALCO CHEMICAL CO
摘要:A polymer having pendant fluorescent groups is prepared by (trans)amidation of a preformed polymer by reaction with an amine-containing organic fluorescent composition of the Formula III Formula III wherein one of R5 and R6 may be hydrogen, and wherein within at least one of R5 and R6, or within R5 and R6 taken together, is an organic fluorescent group wherein the organic fluorescent group includes a polynuclear aromatic ring system. A degree of (trans)amidation derivatization of the polymer is accomplished by heating the admixture of polymer and fluorescent agent for a sufficient period of time. A second amine-containing post-polymerization derivatization agent other than the organic fluorescent composition of Formula III is added to the admixture or to at least a portion of the reaction product of the (trans)amidation derivatization, and is heated therewith to accomplish a degree of sequential or simultaneous post-polymerization derivatization with the second amine-containing post-polymerization derivatization agent.