专利号:US-11447760-B2 优先权日:2020-09-29 标 题 :Special enzyme for galactooligosaccharide production as well as preparation and application thereof 发明人:WANG ZHENGXIANG; NIU DANDAN; TIAN KANGMING 权利人:TIANJIN UNIV OF SCIENCE AND TECHNOLOGY 摘要:A method of using lactase for generating galactooligosaccharide as well as the preparation and an application of the lactase are provided. Lactase (BglD305 derived from Bacillus circulans B2301 and BglD derived from Bacillus circulans ATCC 31382) molecules from two sources are taken as the basis for molecular evolution, so as to obtain new lactase enzyme molecules with high galactooligosaccharide synthesis efficiency and good expression performance. The high-producing strain lactase is further constructed, the lactase can be efficiently synthesized during the submerged fermentation, and the enzyme molecule is secreted into the culture medium, the high-activity enzyme preparation is directly prepared from the fermentation supernatant, and the lactase expression level can achieve 2208 U/mL. As the result, the fermentation manufacturing cost of lactase is reduced, the fermentation manufacturing process is simplified, and the quality of the lactase preparation is improved.
专利号:EP-1694637-A4 优先权日:2003-12-17 标题 :PROCESS FOR SYNTHESIS OF DISULFIDENE
专利号:CN-115193475-B 优先权日:2022-08-11 标题 :Preparation and Application of Catalyst for Synthesis of Fatty Acyl Sulfonate Surfactant
专利号:CN-115193475-A 优先权日:2022-08-11 标 题:Preparation and application of catalyst for fatty acyl sulfonate surfactant synthesis
参考文献:10.1007/bf01871104 摘要:Berry CA, Verkman AS. Osmotic gradient dependence of osmotic water permeability in rabbit proximal convoluted tubule. The Journal of Membrane Biology. 1988 Oct;105(1):33–43. doi: 10.1007/bf01871104. 参考文献:10.1007/bf01869008 摘要:Jirounek P, Rouiller M, Ferrero JD, Straub RW. Role of potassium in the phosphate efflux from mammalian nerve fibers. The Journal of Membrane Biology. 1980 Feb;52(1):75–82. doi: 10.1007/bf01869008.