CAS: 5536-30-1; 5-Amino-2'-Deoxyuridine

上下游产品

5-bromo-2'-deoxyuridine 5-Iodo-2'-deoxyuridine 5-Aminouracil thymidine

📜溴脲苷置于氨体系中,化学反应 44.0H,以65%的收率获得产物5-氨基-2'-脱氧尿苷
参考文献:5-氨基-2'-脱氧尿苷,一种新型的胸苷类似物,用于蛋白质-Dna复合物的高分辨率印迹.
标题:5-氨基-2'-脱氧尿苷,一种新型的胸苷类似物,用于蛋白质-Dna复合物的高分辨率印迹.
摘要:〔公式:见正文〕合成了脱氧胸苷三磷酸的类似物5-氨基-2'-脱氧尿苷5'-三磷酸,发现它是taq Dna聚合酶的底物.dna反应生成的类似物与高锰酸盐发生选择性化学反应.使用ada蛋白/ Ada启动子复合物验证了5-氨基-Du作为干扰探针的使用.5-氨基-Du在干扰印迹中的性能类似于先前描述的类似物5-羟基-Du,但在酶促聚合过程中,前者更容易掺入dna中.
DOI:10.1021/ol026735L

专利信息

专利号:US-4672111-A
优先权日:1985-04-23
标 题:5-azido-deoxyuridine compounds and method of synthesis of the same
发明人：HALEY BOYD E; EVANS ROBERT K
权利人:UNIV WYOMING
摘要:5-azido-2-'-deoxyuridine nucleoside and nucleotide compounds and a method of producing them. The method involves reacting a 5'-deoxyuridine compound with nitrosonium tetrafluoroborate to produce a 5-nitro-deoxyuridine compound. The 5-nitro-deoxyuridine compound is reduced to a 5-amino-deoxyuridine compound in the presence of metallic zinc. The 5-amino-deoxyuridine compound is acidified and reacted with sodium nitrite to produce a 5-diazo-deoxyuridine compound. The diazo-deoxyuridine compound is then reacted with sodium azide to produce a 5-azido-deoxyuridine compound. The latter, in the monophosphate form, is further reacted with diphenylchlorophosphate to produce the photoactive triphosphate, 5-azido-2'-deoxyuridine-5'-triphosphate or 5-azido-dUTP. The photoactive compound, when added to DNA in an in vitro synthesis reaction, produces photoactive DNA.

专利号:EP-1600451-A2
优先权日:1999-11-12
标题 :Synthesis of 2'-deoxy-l-nucleosides

专利号:EP-1600452-A2
优先权日:1999-11-12
标题 :Synthesis of 2'-deoxy-L-nucleosides

专利号:EP-1634888-A2
优先权日:1999-11-12
标题 :Synthesis of 2'-deoxy-L-nucleosides

专利号:WO-8606253-A1
优先权日:1985-04-23
标题:5-azido deoxyuridine compounds and method of synthesis of the same

专利号:EP-0218701-B1
优先权日:1985-04-23
标题 :5-azido deoxyuridine compounds and method of synthesis of the same