CAS: 9001-77-8; Phosphatase Acid

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丙炔基缩水甘油醚 Prop-2-Ynyloxymethyl-Oxirane 18180-30-8

📜1,2-丙二醇,3-溴丙炔置于sodium Iodide Sodium Hydride,盐酸体系中,用 N,N-二甲基甲酰胺,水 作为反应溶剂,化学反应 7.0H,以42%的收率获得产物磷酸酯酶,酸性的,从麦芽所得
参考文献:Wo2006/43654
标题:Wo2006/43654

专利信息

专利号:WO-9516049-A1
优先权日:1993-12-08
标题 :Catalytic chemo-enzymatic asymmetric synthesis of carbohydrates
发明人：WONG CHI-HUEY; SHARPLESS BARRY K
权利人:SCRIPPS RESEARCH INST; WONG CHI HUEY; SHARPLESS BARRY K
摘要:A stereoselective synthesis of carbohydrates employs two key stereoselective steps, viz. an osmium-catalyzed asymmetric dihydroxylation (AD) of an alkene to form an aldol intermediate and an aldolase-catalyzed aldol addition reaction wherein the aldol intermediate is elongated by the addition of a ketone. Moreover, ketoses having four new stereocenters can be synthesized without the use of chiral starting materials. The stereoselectivity of the asymmetric dihydroxylation (AD) reaction is determined by the cis or trans (Z or E) stereoisomeric configuration of the alkene and by the choice of AD-mix, i.e., an AD-mix-α or AD-mix-β. The stereoselectivity of the aldolase-catalyzed addition reaction is determined by the choice of aldolase and by the stereochemistry of the aldol intermediate. Four aldolases with broad substrate specificity are sufficient to produce all possible enantiomeric combinations of the two hydroxymethylene stereocenters formed during the aldol addition reaction. Accordingly, a synthetic scheme combining an osmium-catalyzed asymmetric dihydroxylation (AD) reaction and an aldolase-catalyzed aldol addition reaction enables direct access to a wide range of carbohydrate derivatives containing up to four new hydroxymethylene stereocenters.

专利号:US-8822187-B1
优先权日:2011-04-27
标题:Polypeptides, nucleic acid molecules, and methods for synthesis of triterpenes
发明人：CHAPPELL JOE; NIEHAUS THOMAS D; OKADA SHIGERU; DEVARENNE TIMOTHY P; WATT DAVID S
权利人:CHAPPELL JOE; NIEHAUS THOMAS D; OKADA SHIGERU; DEVARENNE TIMOTHY P; WATT DAVID S; UNIV KENTUCKY RES FOUND
摘要:This application relates to the polypeptides, nucleic acid molecules, vectors, transfected cells, and methods for synthesis of triterpenes, including botryococcene.

专利号:US-5714323-A
优先权日:1991-08-30
标题:Over expression of single-stranded molecules
发明人：OHSHIMA ATUSHI; INOUYE SUMIKO; INOUYE MASAYORI
权利人:UNIV MEDECINE AND DENTISTRY OF
摘要:A method of synthesis of new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or an in vitro synthesis. Replicating vehicles which produce these ss-slDNAs. The ss-slDNAs are described. Uses for these slDNAs are disclosed. They can be used for introducing random mutations, they lend themselves for replication by a variant of the PCR method. They can also be used for regulating gene function. Other uses are disclosed.

专利号:US-5436143-A
优先权日:1992-12-23
标题:Method for enzymatic synthesis of oligonucleotides
发明人：HYMAN EDWARD D
摘要:Enzymatic synthesis of oligonucleotides may be performed in a single vessel without intermediate purification, by the steps of: n (a) combining a nucleotide primer sequence and a blocked nucleotide in the presence of a chain extending enzyme whereby a reaction mixture is formed containing the blocked nucleotide coupled to the nucleotide primer sequence at its 3' end; n (b) inactivating the chain extending enzyme; n (c) removing the blocking group from the primer-blocked nucleotide to form a primer-nucleotide product; and converting any unreacted blocked nucleotide to an unreactive form which is substantially less active as a substrate for the chain extending enzyme than the blocked nucleotide.

专利号:US-5576426-A
优先权日:1988-08-30
标 题:Aldolase catalyzed stereoselective synthesis of arabinohexulose, xyloheptulose, threohexulose and xylohexulose
发明人：WONG CHI-HUEY; DURRWACHTER JOHN R; PEDERSON RICHARD L
权利人:SEARLE & CO
摘要:The present invention relates to aldolase catalyzed stereo-selective synthesis of sugars and compositions of matter comprising arabinohexulose, xyloheptulose, threohexulose, and xylohexulose.

专利号:US-2001049117-A1
优先权日:1998-08-20
标 题 :Analogs of udp-murnac peptides, assays, kits and related methods of their use
发明人：AXELROD HELENA R; BRANSTROM ARTHUR A
摘要:General compositions and methods for detecting Lipid I, Lipid II and peptidoglycan synthesis is disclosed. A method of screening for potential antibacterial agents is provided which requires bacterial membrane preparations or enriched enzyme preparations including at least one bacterial enzyme involved in the synthesis of Lipid I from UDP-MurNAc pentapeptide and undecaprenyl phosphate, at least one bacterial enzyme involved in the synthesis of Lipid II from Lipid I and UDP-GlcNAc and one or more bacterial enzymes involved in the further processing of Lipid II toward the downstream synthesis of peptidoglycan. The methods disclosed herein further provides a labeled UDP-MurNAc-peptide capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid I and a labeled UDP-GlcNAc capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid II. Conditions for further processing of Lipid II toward the downstream synthesis of peptidoglycan are also discribed.