专利号:US-5783685-A 优先权日:1988-12-16 标题 :Chemical preparation of high specific activity nucleotide probes 发明人:BAHL CHANDER; MENDOZA LEOPOLDO 权利人:ORTHO DIAGNOSTIC SYSTEMS INC 摘要:Methods of chemical synthesis of labeled nucleotide probes are described. The methods comprise providing a first target recognition moiety comprising a nucleotide sequence of at least about 15 nucleotide bases including at least one 5' end and 3' end. The first target recognition moiety is chemically altered to contain a carboxyl group. A second signal generating moiety comprising a hydroxyl group is provided. The carboxyl group of the first target recognition moiety is chemically reacted with hydroxyl group of the second signal generating moiety to chemically join the two moieties and produce said labeled nucleotide probe.
专利号:US-5538872-A 优先权日:1988-12-21 标题 :Method of preparing nucleotide probes using a bridging complement 发明人:BAHL CHANDER; MENDOZA LEOPOLDO; MCMAHON FRANK J 权利人:ORTHO DIAGNOSTIC SYSTEMS INC 摘要:The present invention provides labeled nucleotide probes and a procedure for synthesis of the labeled nucleotide probe by a coupling or joinder of two components of the probe, namely a target recognition sequence moiety and a signal generating moiety, through the use of a bridging complement that holds the two moieties in such a position as to allow formation of a sugar-phosphate chemical linkage between the 3' end of one moiety and the 5' end of the other moiety.
专利号:US-7851658-B2 优先权日:2004-08-17 标 题:Palladium-catalyzed carbon-carbon bond forming reactions 发明人:LIU DAVID R; KANAN MATTHEW W; ROZENMAN MARY M 权利人:HARVARD COLLEGE 摘要:A novel palladium-mediated carbon-carbon bond forming reaction has been discovered using DNA-templated chemistry. The inventive reaction involves the palladium-mediated coupling of a terminal alkyne with an alkene to form an enone. A catalytic amount of palladium may be used in the reaction if an oxidant is present. The reactions is also compatible with a variety of organic solvent as well as aqueous solution. Both intermolecular and intramolecular reactions have been demonstrated. This novel carbon-carbon bond forming reaction is particularly useful in the synthesis of macrocycles. Kits, reagents, catalysts, solvents, oxidants, salts, acids, instructions, and other materials useful in the practice of the inventive reaction are also provided.
专利号:US-6316669-B1 优先权日:1998-12-18 标 题:Bis-amido polybiguanides and the use thereof to disinfect contact lenses and preserve pharmaceutical compositions 发明人:PARK JOONSUP; MCQUEEN NATHANIEL D 权利人:ALCON MFG LTD 摘要:Bis-amido polybiguanides and their use as antimicrobial agents in pharmaceutical compositions are disclosed. A method of synthesis of bis-amido polybiguanides is also disclosed. The bis-amido polybiguanides are useful in the preservation of pharmaceutical compositions, particularly ophthalmic and otic pharmaceutical compositions and compositions for treating contact lenses. The compounds are especially useful for disinfecting/cleaning contact lenses.
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