CAS: 643-13-0; D-Fructose-6-Phosphate

相似化合物

17187-72-3 56-73-5 20479-58-7

上下游产品

D-葡萄糖-6-磷酸 D-Glucose 6-Phosphate 56-73-5
果糖 D-Fructose 57-48-7
(2,3-二羟基-4-氧代-丁氧基)膦酸 D-Erythrose 4-Phosphate 585-18-2

📜D-葡萄糖-6-磷酸置于escherichia Coli 5-Keto-4-Deoxyuronate Isomerase体系中,用 Aq. Buffer 作为反应溶剂,化学反应生成 果糖-6-磷酸
参考文献:大肠杆菌中的一种新型铜氨型磷酸葡萄糖异构酶.
标题:大肠杆菌中的一种新型铜氨型磷酸葡萄糖异构酶.
摘要:背景技术大肠杆菌细胞包含来自果胶降解土壤细菌的5-酮-4-脱氧尿酸异构酶(kdui)的同源物,但是从未证明大肠杆菌蛋白(o-Kdui)的催化活性.方法将已知的大肠杆菌o-Kdui三维结构与糖转化酶的可用结构进行比较.基于该分析的结果,针对一组d-糖及其衍生物测试了重组o-Kdui的糖异构化活性.结果o-Kdui的三维结构与激烈热球菌铜杯型磷酸葡萄糖异构酶具有相似性.根据这种相似性,发现o-Kdui催化6-磷酸葡萄糖和6-磷酸果糖的相互转化,并且催化葡萄糖醛酸酯转化为果糖酸酯的效率较低.o-Kdui在晶体中为六聚体,但代表溶液中非活性六聚体和活性二聚体的混合物,并包含紧密结合的zn2 +离子.稀释,底物结合和zn2 +的去除将六聚体⇆二聚体平衡移至二聚体.结论我们的发现将o-Kdui鉴定为大肠杆菌中一种新型的磷酸糖异构酶,其活性可能受寡聚结构的改变所调节.一般意义超过5700个蛋白质序列被标
DOI:10.1016/j.Bbagen.2020.129601

专利信息

专利号:US-5824472-A
优先权日:1986-03-24
标 题 :Process for the synthesis of sugar nucleotides using recombinant-DNA methods
发明人：BETLACH MICHAEL R; DOHERTY DANIEL H; VANDERSLICE REBECCA W
权利人:MONSANTO CO
摘要:A recombinant-DNA mediated method for the synthesis of sugar nucleotides is disclosed. This method utilizes portable DNA sequences capable of directing the microbial synthesis of various enzymes that catalyze the synthesis of sugar nucleotides, including UDP-glucose, UDP-glucuronic acid and GDP-mannose. The sugar moieties of these sugar nucleotides may subsequently be incorporated into industrially-useful polysaccharides such as xanthan gum. It has been found that vectors containing the portable DNA sequences described herein are capable both of causing sugar nucleotide production in microorganisms previously incapable of such synthesis and of causing increased sugar nucleotide production in organisms capable of synthesizing small quantities of these compounds. In particular, plasmids pAS7, pAS9 and pTS13 are disclosed. These plasmids are capable of directing sugar nucleotide synthesis in various hosts, including Xanthomonas sp. such as X. campestris and other organisms such as E. coli and various Pseudomonas sp.

专利号:US-2012190064-A1
优先权日:2004-10-01
标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
发明人：KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA
权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP
摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

专利号:US-7229797-B1
优先权日:1999-08-20
标 题:Enzymatic synthesis of deoxyribonucleosides
发明人：TISCHER WILHELM; IHLENFELDT HANS-GEORG; BARZU OCTAVIAN; SAKAMOTO HIROSHI; PISTOTNIK ELISABETH; MARLIERE PHILIPPE; POCHET SYLVIE
权利人:PASTEUR INSTITUT
摘要:The present invention relates to a method for the in vitro enzymatic synthesis of deoxyribonucleosides and enzymes suitable for this method.

专利号:US-2006211083-A1
优先权日:2005-01-21
标题:Products and processes for in vitro synthesis of biomolecules
发明人：KATZEN FEDERICO; KUDLICKI WIESLAW; FLETCHER JULIA
权利人:KATZEN FEDERICO; KUDLICKI WIESLAW; FLETCHER JULIA
摘要:Provided herein are products and processes for efficiently synthesizing biomolecules in vitro using cell-free extracts derived from mammalian cells and insect cells. In an embodiment, provided is a process for preparing a cell-free extract from insect cells and mammalian cells that efficiently synthesizes post-translationally modified target proteins (e.g., glycosylated target proteins). In some embodiments, a ribonucleic acid is synthesized in vitro that comprises a cap, a 5′ untranslated region comprising an 18S rRNA binding ribonucleotide sequence, and a target ribonucleotide sequence. It has been determined that such ribonucleic acids result in efficient in vitro synthesis of a target protein using cell-free extracts derived from non-rabbit mammalian cells and insect cells.

专利号:WO-2024165611-A1
优先权日:2023-02-07
标题:Production of a disaccharide and/or milk oligosaccharide by a cell with reduced synthesis of udp-glcnac
发明人：BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES
权利人:INBIOSE NV
摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide wherein synthesis of UDP-N-acetylglucosamine in said cell is rendered less functional. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide using said cell as well as the purification of said disaccharide and/or oligosaccharide like a milk oligosaccharide.

专利号:US-5302520-A
优先权日:1990-09-25
标 题:Enzymatic synthesis of isotopically labeled carbohydrates
发明人：GOUX WARREN J
权利人:UNIV TEXAS
摘要:The present invention relates to a general method of enzymatic synthesis of isotopically labeled carbohydrates, sugars and nucleosides. Labeled citric acid cycle intermediates, amino acids and ribose mononucleotides may be rapidly and conveniently synthesized from labeled pyruvate, lactate or L-alanine. The method employs a novel nicotinamide dinucleotide regeneration system which permits use of low NADH levels. The method may be manipulated to allow labeling at a variety of carbon/hydrogen sites.