CAS: 104809-16-7; 7-Methylguanosine 5'-Diphosphate Sodium Salt

专利信息

专利号:US-4710566-A
优先权日:1984-12-27
标题:Purification of the messenger RNA cap-binding protein
发明人：KOZARICH JOHN W; RHOADS ROBERT E
权利人:UNIV YALE
摘要:The synthesis of a new affinity medium, the p-aminophenyl-γ-ester of 7-methylguanosine-5'-triphosphate coupled to Sepharose, for use in affinity chromatography is claimed. An improved method in terms of speed and purity for the isolation of messenger ribonucleic acid cap-binding protein is claimed. The synthesis of the p-aminophenyl-γ-ester of 7-methylguanosine-5'-triphosphate is described.

专利号:US-8344118-B2
优先权日:2007-10-31
标 题 :Preparation and isolation of 5′ capped MRNA
发明人：KORE ANILKUMAR; MUTHIAN SHANMUGASUNDARAM; CHARLES IRUDAYA
权利人:APPLIED BIOSYSTEMS LLC; KORE ANILKUMAR; MUTHIAN SHANMUGASUNDARAM; CHARLES IRUDAYA
摘要:The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.

专利号:US-2023130423-A1
优先权日:2020-02-12
标题:Novel mrna 5'-end cap analogs, rna molecule incorporating the same, uses thereof and method of synthesizing rna molecule or peptide
发明人：WARMINSKI MACIN; SIKORSKI PAWEL; KOWALSKA JOANNA; JEMIELITY JACEK
权利人:UNIV WARSZAWSKI; EXPLORNA THERAPEUTICS SP Z O O
摘要:The invention relates to new mRNA 5′ end cap analogs, RNA molecules containing them, their uses and methods for their in vitro synthesis, as well as a method for protein or peptide synthesis in vitro or in cell cultures, which method encompasses translation of the RNA molecule.

专利号:WO-2023199261-A1
优先权日:2022-04-13
标 题:Rna molecule containing modified cap analogs at the 5 ' end, use of rna molecule in in vitro protein or peptide synthesis, rna molecule for use in medicine, and use of modified cap analogs for rna capping
发明人：JANKOWSKA-ANYSZKA MARZENA; PIECYK KAROLINA; GRZELA RENATA; DARZYNKIEWICZ EDWARD
权利人:UNIV WARSZAWSKI
摘要:The subject of the invention is a RNA molecule containing a compound defined by formula (II) at the 5' terminus. The invention also includes an in vitro method of protein or peptide synthesis in cell cultures, wherein said method involves translation of a RNA molecule, use of a RNA molecule in medicine and the use of new cap analogues in capping.

专利号:WO-9808865-A1
优先权日:1996-08-30
标 题:CAP-BINDING DOMAIN OF HUMAN EUKARYOTIC PROTEIN SYNTHESIS INITIATION FACTOR eIF-4E AND THE USE THEREOF
发明人：GOSS DIXIE J; FRIEDLAND DIANA
权利人:LIFE TECHNOLOGIES INC; GOSS DIXIE J; FRIEDLAND DIANA
摘要:Binding of eIF-4E to the 5'm7G cap structure of eukaryotic mRNA signals the initiation of protein synthesis. In the present invention, the binding site cap-binding domain of eIF-4E was identified as the region containing the sequence Trp?113 to Arg122¿. Thus, in a first preferred embodiment the present invention provides a truncated peptide of the cap binding protein consisting essentially of the mRNA cap-binding domain. Specifically, the invention concerns eIF-4E cap binding peptide and its variants. In additional preferred embodiments, the invention provides an isolated nucleic acid molecule which encodes a truncated peptide of the eIF-4E protein consisting essentially of the mRNA cap-binding domain of eIF-4E; a recombinant vector and host cell comprising the above-described nucleic acid molecule; and a recombinant peptide produced thereby. The invention further provides the truncated or recombinant peptides immobilized on a solid support, a resin comprising the truncated or recombinant peptides immobilized on a solid support, methods and kits for isolating a capped mRNA molecule, and methods and kits for producing a full-length cDNA molecule.

专利号:US-5872011-A
优先权日:1997-06-13
标 题:Crystal of a protein-ligand complex containing an N-terminal truncated eIF4E, and methods of use thereof
发明人：BURLEY STEPHEN K; SONENBERG NAHUM; MARCOTRIGIANO JOSEPH; GINGRAS ANNE-CLAUDE
权利人:UNIV ROCKEFELLER; UNIV MCGILL
摘要:A detailed three-dimensional structure for the least abundant of the general translation initiation factors in eukaryotes, eIF4E, complexed with a ligand is disclosed. The novel N-terminal truncated eIF4Es which were constructed so as to omit a significant portion of the flexible N-terminal tail of the eIF4E are also part of the present invention. In addition, the crystals of the protein-ligand complexes containing the N-terminal truncated eIF4Es are also included. Furthermore, methods of identifying antagonists of the eIF4E protein which can be used to regulate protein synthesis in cells are also disclosed.