CAS: 4178-93-2; (S)-2-Amino-4-Methyl-N-(4-Nitrophenyl)Pentanamide

该化合物是一种合成化合物,通常用于生物化学应用,特别是用于研究蛋白性酶,是一种氨酸衍生物,具体地说是一种在和p-nitroaniline之间形成的浸泡物联结物,其特点是它能够作为各种酶的基质,使研究人员能够调查酶活动和动能.Leucine p-nitroanilide通常是一种白色到非白晶状固体,并且可溶于有机溶剂,便于其在实验室环境中使用.p-notroanilide组的存在提供了一种染色性属性,通过色度分析分析检测出酶活动.该复合物在标准实验室条件下保持稳定,成为试验程序的可靠选择,因此也值得注意,与许多化学物质一样,应当注意适当的处理和安全防范措施,因为如果进行或管理不当,可能会对健康造成风险.

结构式图片

物理性质

MSDS等安全信息

欧盟法规

ECHA物质C&L通报REACH预注册

上下游产品

CAS号1174-27-2 z-leu-pna | CAS号114684-52-5 N-tert-butoxyca... | CAS号3303-55-7 L-苯丙氨酰-L-亮氨酸 | CAS号61-90-5 L-亮氨酸 | CAS号100-01-6 对硝基苯胺 | CAS号14486-16-9 H-MET-LEU-OH

合成工艺路线路线简述

    📜N-Tert-Butoxycarbonyl-L-Leucine P-Nitroanilide置于盐酸体系中,用 乙醇,水 作为反应溶剂,以78.3 %的收率获得产物l-亮氨酸-4-硝基苯胺
    参考文献:Cn115974752
    标题:Cn115974752

    海关参考信息

    专利信息


    专利号:US-6448058-B1
    优先权日:1997-09-12
    标 题:Methods for solid phase synthesis of mercapto compounds and derivatives, combinatorial libraries thereof and compositions obtained thereby
    发明人：PATEL DINESH V; NGU KHEHYONG; ZHOU JIANPING
    权利人:VERSICOR INC
    摘要:Methods of preparing combinatorial libraries of mercapto (thiol) compounds them and compositions obtained therefrom are disclosed. The compounds are synthesized on a solid support. Following synthesis, the compounds are optionally cleaved from the support. One such method of synthesis involves attack of an S-protected nucleophile on a resin functionalized with a leaving group. The invention also provides for screening the mercapto compounds for bioactive compounds; in particular, for inhibitors of MMPs.

    专利号:US-4634671-A
    优先权日:1982-09-18
    标 题 :Water-soluble cross-linked polymer of lysyl endopeptidase, process for preparing same and use of same
    发明人：SAKATA YOSHITSUGU; SHINTANI AKINORI; MATSUO TETSUYA; SUGIYAMA HARUHIKO; TOKIOKA NOBUYUKI
    权利人:WAKO PURE CHEM IND LTD
    摘要:A water-soluble cross-linked polymer of the enzyme lysyl endopeptidase produced by Achromobacter lyticus and a process for preparing the polymer as well as a semi-synthesis of human insulin using the polymer.

    专利号:CN-102936207-B
    优先权日:2011-08-15
    标 题:A new method for the synthesis of an important biochemical reagent L-leucine-4-nitroaniline hydrochloride

    专利号:EP-0367302-A2
    优先权日:1982-04-23
    标 题:Process for semi-synthesis of human insulin, water-soluble cross-linked Achromobacter protease I for use therein and a process for preparing the same

    专利号:EP-0092829-A2
    优先权日:1982-04-23
    标题 :Process for semi-synthesis of human insulin and alkaline protease for use therein

    专利号:EP-3027591-B1
    优先权日:2013-07-30
    标 题:Novel derivatives of a 3,4-dihydroisoquinoline-3-carboxylic acid having anti-cancer properties, a method for their synthesis, pharmaceutical compositions comprising said derivatives, and their use
    发明人：SOLECKA JOLANTA; KAWECKI ROBERT; POSTEK MAGDALENA; PYPOWSKI KRZYSZTOF; ZIEMSKA JOANNA; GUSPIEL ADAM
    权利人:NARODOWY INSTYTUT ZDROWIA PUBLICZNEGO PANSTWOWY ZAKL HIGIENY; UNIV PRZYRODNICZO HUMANISTYCZNY W SIEDLCACH

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    ✅ COA系统入驻 | 共享模式

    主要参考文献


    1: Lin SH, Van Wart HE. Effect of cryosolvents and subzero temperatures on the hydrolysis of L-leucine-p-nitroanilide by porcine kidney leucine aminopeptidase. Biochemistry. 1982 Oct 26;21(22):5528-33. Japanese. doi: 10.1007/s00775-011-0843-2. Epub 2011 Sep 15. doi: 10.1016/j.ijbiomac.2013.12.023. Epub 2013 Dec 22. doi: 10.1002/elps.201400283. Epub 2014 Dec 17. doi: 10.4061/2010/517283.
    8: Hegazi FZ, Abo-Elnaga IG. Proteolytic activity of crude cell-free extract of Lactobacillus casei and Lactobacillus plantarum. Nahrung. 1987;31(3):225-32. doi: 10.1016/j.jinsphys.2012.03.009. Epub 2012 Mar 20. doi: 10.3168/jds.2010-3738. doi: 10.1016/j.mvr.2011.05.008. Epub 2011 Jun 12. Review. doi: 10.1007/s10637-010-9391-9. Epub 2010 Jan 29. doi: 10.1021/am1005095.
    20: Cui SX, Qu XJ, Gao ZH, Zhang YS, Zhang XF, Zhao CR, Xu WF, Li QB, Han JX. Targeting aminopeptidase N (APN/CD13) with cyclic-imide peptidomimetics derivative CIP-13F inhibits the growth of human ovarian carcinoma cells. Cancer Lett. 2010 Jun 28;292(2):153-62. doi: 10.1016/j.canlet.2009.11.021. Epub 2009 Dec 29.

    合成参考文献


    参考文献:10.1074/jbc.m106950200
    摘要:Cahan R, Axelrad I, Safrin M, Ohman DE, Kessler E. A secreted aminopeptidase of Pseudomonas aeruginosa. Identification, primary structure, and relationship to other aminopeptidases. J Biol Chem. 2001 Nov 23;276(47):43645–52. doi: 10.1074/jbc.m106950200.
    参考文献:10.1007/s11356-018-2133-9
    摘要:Ansari FA, Khan AA, Mahmood R. Protective effect of carnosine and N-acetylcysteine against sodium nitrite-induced oxidative stress and DNA damage in rat intestine. Environmental Science and Pollution Research. 2018 May 04;25(20):19380–92. doi: 10.1007/s11356-018-2133-9.
    参考文献:10.1007/s00394-004-0466-2
    摘要:Zaiger G, Nur T, Barshack I, Berkovich Z, Goldberg I, Reifen R. Vitamin A exerts its activity at the transcriptional level in the small intestine. European Journal of Nutrition. 2004 Jan 28;43(5):259–66. doi: 10.1007/s00394-004-0466-2.
    参考文献:10.1007/978-1-60327-058-8_21
    摘要:Proudfoot M, Kuznetsova E, Sanders SA, Gonzalez CF, Brown G, Edwards AM, Arrowsmith CH, Yakunin AF. High throughput screening of purified proteins for enzymatic activity. Methods Mol Biol. 2008;426():331–41. doi: 10.1007/978-1-60327-058-8_21.
    摘要:Landau R, Laverrière A, Bischof P, Irion O, Morales M, Cohen M. Alteration of circulating Placental Leucine Aminopeptidase (P-LAP) activity in preeclampsia. Neuro Endocrinol Lett. 2010;31(1):63–6.
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