CAS: 31712-49-9; (S)-5-Hydroxy-2-(3-Hydroxy-4-Methoxyphenyl)-7-(((2S,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Oxy)Chroman-4-One

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hesperidin (S)-2,3-dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-benzopyroneS)-5-acetoxy-2-(3-acetoxy-4-methoxy-phenyl)-7-(tetra-O-acetyl-β-D-glucopyranosyloxy)-chroman-4-one(S)-5-acetoxy-2-(3-acetoxy-4-methoxy-phenyl)-7-(tetra-O-acetyl-β-D-glucopyranosyloxy)-chroman-4-one

📜橙皮甙置于α-L-Rhamnosidase体系中,用 Aq. Buffer 用作溶剂,化学反应 0.58H,反应生成橙皮素 7-O-葡萄糖甙
参考文献:Purification And Characterization Of A Naringinase From Aspergillus Aculeatus Jmudb058
标题:Purification And Characterization Of A Naringinase From Aspergillus Aculeatus Jmudb058
摘要:A Naringinase From Aspergillus Aculeatus Jmudb058 Was Purified,Identified,And Characterized. This Naringinase Had A Molecular Mass (Mw) Of 348 Kda And Contained Four Subunits With Mws Of 100,95,84,And 69 Kda. Mass Spectrometric Analysis Revealed That The Three Larger Subunits Were Beta-D-Glucosidases And That The Smallest Subunit Was An Alpha-L-Rhamnosidase. The Naringinase And Its Alpha-L-Rhamnosidase And Beta-D-Glucosidase Subunits All Had Optimal Activities At Approximately Ph 4 And 50 Degrees C,And They Were Stable Between Ph 3 And 6 And Below 50 Degrees C. This Naringinase Was Able To Hydrolyze Naringin,Aesculin,And Some Other Glycosides. The Enzyme Complex Had A K-M Value Of 0.11 Mm And A K(Cat)/k-M Ratio Of 14 034 S(-1) Mm(-1) For Total Naringinase. Its Alpha-L-Rhamnosidase And Beta-D-Glucosidase Subunits Had K-M Values Of 0.23 And 0.53 Mm,Respectively,And K(Cat)/k-M Ratios Of 14 146 And 7733 S(-1) M(-1),Respectively. These Results Provide In-Depth Insight Into The Structure Of The Naringinase Complex And The Hydrolyses Of Naringin And Other Glycosides.
Doi:10.1021/jf303512Q