📜辛酰叠氮化物 以 吡啶,甲苯 用作溶剂,化学反应 22.0H,反应生成甲基6-O(N-庚甲酰)-α-D-葡萄糖苷 参考文献:Chimie Des Sucres Sans Groupements Protecteurs-Ii Reactions Selectives D'Addition Du-Glucose,Du-Galactose Et De-Glycosylamines A Des Heterocumulenes 标题:Chimie Des Sucres Sans Groupements Protecteurs-Ii Reactions Selectives D'Addition Du-Glucose,Du-Galactose Et De-Glycosylamines A Des Heterocumulenes 摘要: Doi:10.1016/s0040-4020(01)85430-2
专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-2001049117-A1 优先权日:1998-08-20 标 题 :Analogs of udp-murnac peptides, assays, kits and related methods of their use 发明人:AXELROD HELENA R; BRANSTROM ARTHUR A 摘要:General compositions and methods for detecting Lipid I, Lipid II and peptidoglycan synthesis is disclosed. A method of screening for potential antibacterial agents is provided which requires bacterial membrane preparations or enriched enzyme preparations including at least one bacterial enzyme involved in the synthesis of Lipid I from UDP-MurNAc pentapeptide and undecaprenyl phosphate, at least one bacterial enzyme involved in the synthesis of Lipid II from Lipid I and UDP-GlcNAc and one or more bacterial enzymes involved in the further processing of Lipid II toward the downstream synthesis of peptidoglycan. The methods disclosed herein further provides a labeled UDP-MurNAc-peptide capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid I and a labeled UDP-GlcNAc capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid II. Conditions for further processing of Lipid II toward the downstream synthesis of peptidoglycan are also discribed.
专利号:US-6600029-B1 优先权日:1995-12-19 标题:Metabolic engineering of polyhydroxyalkanoate monomer synthases 发明人:SHERMAN DAVID H; WILLIAMS MARK D; XUE YONGQUAN 权利人:UNIV MINNESOTA 摘要:A novel pathway for the synthesis of polyhydroxyalkanoate is provided. A method of synthesizing a recombinant polyhydroxyalkanoate monomer synthase is also provided. These recombinant polyhydroxyalknoate synthases are derived from multifunctional fatty acid synthases or polyketide synthases and generate hydroxyacyl acids capable of polymerization by a polyhydroxyalknoate synthase.
专利号:US-2003194784-A1 优先权日:2001-04-17 标题:DNA encoding methymycin and pikromycin 发明人:SHERMAN DAVID H; LIU HUNG-WEN; XUE YONGQUAN; ZHAO LISHAN 摘要:A novel pathway for the synthesis of polyhydroxyalkanoates is provided. A method of synthesizing a recombinant polyhydroxyalkanoate monomer synthase is also provided. These recombinant polyhydroxyalkanoate synthases are derived from multifunctional fatty acid synthases or polyketide synthases and generate hydroxyacyl acids capable of polymerization by a polyhydroxyalkanoate synthase. Also provided is a biosynthetic gene cluster for methymycin and pikomycin as well as a biosynthetic gene cluster for desosamine.
专利号:US-6265202-B1 优先权日:1998-06-26 标 题 :DNA encoding methymycin and pikromycin 发明人:SHERMAN DAVID H; LIU HUNG-WEN; XUE YONGQUAN; ZHAO LISHAN 权利人:UNIV MINNESOTA 摘要:A novel pathway for the synthesis of polyhydroxyalkanoates is provided. A method of synthesizing a recombinant polyhydroxyalkanoate monomer synthase is also provided. These recombinant polyhydroxyalkanoate synthases are derived from multifunctional fatty acid synthases or polyketide synthases and generate hydroxyacyl acids capable of polymerization by a polyhydroxyalkanoate synthase. Also provided is a biosynthetic gene cluster for methymycin and pikomycin as well as a biosynthetic gene cluster for desosamine.
专利号:US-2009209032-A1 优先权日:2004-10-01 标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
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合成参考文献
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