专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-7282337-B1 优先权日:2006-04-14 标题:Methods for increasing accuracy of nucleic acid sequencing 发明人:HARRIS TIMOTHY D 权利人:HELICOS BIOSCIENCES CORP 摘要:The invention provides methods for improving the accuracy of a sequencing-by-synthesis reaction by sequencing at least a portion of a template and at least a portion of template complementary sequence.
专利号:US-2010203524-A1 优先权日:2008-10-31 标题:Polymerases and methods of use thereof 发明人:EFCAVITCH J WILLIAM; BOWERS JAYSON L; BUZBY PHILIP R; THOMPSON JOHN F 权利人:HELICOS BIOSCIENCES CORP 摘要:The invention generally relates to polymerases for efficient and controlled sequencing-by-synthesis reactions. In certain embodiments, the invention provides a polymerase enzyme including at least one mutation that enhances ability of the polymerase as compared to a wild-type polymerase to incorporate a nucleotide into a nascent strand of DNA or cDNA including at least one modified nucleotide.
专利号:US-2015159210-A1 优先权日:2006-04-14 标 题:Methods for Increasing Accuracy of Nucleic Acid Sequencing 发明人:HARRIS TIMOTHY D; LANDER ERIC S 权利人:HARRIS TIMOTHY D; LANDER ERIC S 摘要:The invention provides methods for improving the fidelity of a sequencing-by-synthesis reaction by resequencing at least a portion of a nucleic acid template.
专利号:US-2009305248-A1 优先权日:2005-12-15 标 题:Methods for increasing accuracy of nucleic acid sequencing 发明人:LANDER ERIC G; LAPIDUS STANLEY N 权利人:LANDER ERIC G; LAPIDUS STANLEY N 摘要:The invention provides methods for improving the fidelity of a sequencing-by-synthesis reaction by resequencing at least a portion of a nucleic acid template.
专利号:US-2009209032-A1 优先权日:2004-10-01 标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
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合成参考文献
参考文献:10.1007/s11743-000-0147-7 摘要:Smith DL. Prediction of cloth wetting times from diffusion constants of alcohol ethoxylate mixtures. J Surfact & Detergents. 2000 Oct;3(4):483–90. doi: 10.1007/s11743-000-0147-7. 参考文献:10.1016/s0076-6879(00)12909-x 摘要:Terstappen GC, Futerman AH, Schwarz A. Cloud-point extraction of gangliosides using nonionic detergent C14EO6. Methods Enzymol. 2000;312():187–96. doi: 10.1016/s0076-6879(00)12909-x.