专利号:US-2023313255-A1 优先权日:2020-07-15 标题:Massively Parallel Enzymatic Synthesis of Polynucleotides 发明人:HORGAN ADRIAN; LACHAIZE HENRI; VERARDO DOMIANO; GODRON XAVIER 权利人:DNA SCRIPT 摘要:The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.
专利号:US-2011195450-A1 优先权日:2005-09-27 标 题 :In Vitro Protein Synthesis Systems for Membrane Proteins that Include Adolipoproteins and Phospholipid Adolipoprotein Particles 发明人:KUDLICKI WIESLAW; FLETCHER JULIA; KATZEN FEDERICO 权利人:LIFE TECHNOLOGIES CORP 摘要:In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.
专利号:WO-2007038755-A1 优先权日:2005-09-27 标 题:In vitro protein synthesis systems for membrane proteins that include apolipoproteins and phospholipid-apolipoprotein particles 发明人:KUDLICKI WIESLAW; FLETCHER JULIA; KATZEN FEDERICO 权利人:INVITROGEN CORP; KUDLICKI WIESLAW; FLETCHER JULIA; KATZEN FEDERICO 摘要:In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.
专利号:WO-2025056767-A1 优先权日:2023-09-15 标题 :Process for the preparation of enantiomerically enriched aliphatic amines 发明人:BOU HAMDAN FARHAN; GODINEAU EDOUARD; SMEJKAL TOMAS; DUMEUNIER RAPHAEL; BOUSSEMGHOUNE MOHAMED ABDELOUAHAB; BLUM MATHIAS 权利人:SYNGENTA CROP PROTECTION AG 摘要:The present invention relates to a process for the preparation of enantiomerically enriched cyclobutylamines of formula (I) from α-tertiary cyclobutanones and reacting said enantiomerically enriched cyclobutylamines to enantiomerically enriched cyclobutylamides. Such compounds are useful intermediates in the synthesis of microbiocidal thiazole compounds.
专利号:WO-2024141628-A1 优先权日:2022-12-31 标 题:Variable viscosity inks for inkjet delivery of enzyme reagents 发明人:HORGAN ADRIAN; VERARDO DAMIANO; ADELIZZI BEATRICE 权利人:DNA SCRIPT 摘要:The invention is directed to formulations of terminal deoxynucleotidyl transferases (TdTs) inks employing variable viscosity modifiers and their use in inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate.
专利号:US-2010233782-A1 优先权日:2005-09-27 标 题:Isolated phospholipid-protein particles 发明人:KATZEN FEDERICO; FLETCHER JULIA; KUDLICKI WIESLAW; BEECHEM JOSEPH; WANG LILIN 权利人:LIFE TECHNOLOGIES CORP 摘要:Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHCâ€?) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.
1: Li ZY, Zhang ZC, Zhang L, Leng L. Enantioselective degradation and chiral stability of phenthoate in soil. Bull Environ Contam Toxicol. 2007 Aug;79(2):153-7. Epub 2007 May 18. Japanese.
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