CAS: 67-42-5; 3,12-Bis(Carboxymethyl)-6,9-Dioxa-3,12-Diazatetradecanedioic Acid

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13368-13-3 93-62-9 150-25-4

上下游产品

CAS号929-59-9 1,8-二氨基-3,6-二氧杂辛烷 | CAS号79-11-8 氯乙酸

1,8-二氨基-3,6-二氧杂辛烷,氯乙酸置于sodium Hydroxide体系中,化学反应生成 乙二醇双(2-氨基乙基醚)四乙酸
参考文献:Us2709178
标题:Us2709178

专利信息

专利号:WO-9624694-A1
优先权日:1995-02-10
标 题:Method and kit for fluorometric analysis of enzymes catalyzing synthesis of nucleic acids
发明人：CHAVAN SURENDRA J; PROCHASKA HANS J
权利人:SLOAN KETTERING INST CANCER
摘要:This invention provides a method for detecting in a sample an enzyme which catalyzes the synthesis of a double-stranded nucleic acid molecule from a nucleic acid template which comprises contacting the sample with a suitable fluorophore which selectively binds to double-stranded DNA. Also provided is a method for determining in a sample the activity of such an enzyme. This invention also provides a method for detecting an RNA-DNA heteroduplex in a sample which comprises contacting the sample with a suitable fluorophore which selectively binds to double-stranded DNA. This method for detecting an RNA-DNA heteroduplex may further comprise quantitatively determining detected RNA-DNA heteroduplex. This invention also provides a method for detecting in a sample en enzyme which catalyzes the synthesis of and RNA-DNA heteroduplex from a nucleic acid template, as well as a method of detecting the activity of such an enzyme in a sample. This invention further provides a method for detecting the viral load of HIV in a sample. Also provided is a method for diagnosing an HIV infection in a subject, and for monitoring the progression of an HIV infection in a subject. Also provided is a method for determining the viral load of HIV in a subject infected with HIV. This invention further provides a method for identifying whether a substance inhibits reverse transcriptase. Finally, this invention provides a kit for assaying an enzyme which catalyzes the synthesis of a double-stranded nucleic acid molecule from a nucleic acid template.

专利号:US-2012058520-A1
优先权日:2009-05-14
标 题:Method for synthesis of double-stranded dna corresponding to rna, and method for amplification of the dna
发明人：HAYASHIDA YUKINOBU
权利人:HAYASHIDA YUKINOBU; WAKO PURE CHEM IND LTD
摘要:An object of the present invention is to provide an inexpensive and simple method for synthesis of a double-stranded DNA corresponding to a particular RNA, and a method for amplification of the aforementioned double-stranded DNA. The present invention relates to a method for synthesis of a double-stranded DNA having a nucleotide sequence corresponding to template RNA having polyA, comprising step 1 in which reverse transcription reaction of template RNA is carried out employing oligo(dT)primer to which DNA fragment having a known sequence has been added at the 5′-terminal, to obtain a single-stranded DNA, and step 2 in which double strand formation reaction of single-stranded DNA obtained in step 1 is carried out employing a random primer to which DNA fragment having a known sequence has been added at the 5′-terminal, in the presence of polymerase which does not have 3′→5′ exonuclease activity nor strand displacement activity, to obtain a double-stranded DNA, as well as a method for amplification of a double-stranded DNA having a nucleotide sequence corresponding to template RNA having polyA, further comprising step 3 in which PCR reaction is carried out using the obtained double-stranded DNA.

专利号:US-6329543-B1
优先权日:1999-05-06
标题:Process for synthesis of isobornyl (Meth) acrylate
发明人：KNEBEL JOACHIM; SAAL DORIS
权利人:ROEHM GMBH
摘要:The present invention relates to processes for synthesis of isobornyl (meth)acrylate by reacting camphene with (meth)acrylic acid in the presence of sulfuric acid and at least one compound having inhibiting action. Aqueous sulfuric acid with an acid concentration in the range of 65 to 85 wt % is used. Isobornyl (meth)acrylate can advantageously be distilled from a mixture containing sulfuric acid in the presence of 2,6-di-tert-butyl-alpha-(dialkylamino)-p-cresol.

专利号:US-5386019-A
优先权日:1992-01-15
标 题 :Synthesis of inhibitors of calmodulin-mediated enzymes including KS-501, KS-502 and their enantiomers
发明人：DANISHEFSKY SAMUEL J; DUSHIN Russell; HAIT WILLIAM N
权利人:UNIV YALE
摘要:The total synthesis of a group of compounds with inhibitory effects on calmodulin-mediated enzyme activities has been accomplished. Among these synthesized compounds are KS-501 and KS-502. Other compounds that have been synthesized by the described scheme are ent-KS-501 and ent-KS-502 which are enantiomers of KS-501 and KS-502 and which also have inhibitory effects on calmodulin-mediated enzyme activities.

专利号:US-5674729-A
优先权日:1991-06-24
标 题 :De novo cell-free synthesis of picornavirus
发明人：WIMMER ECKARD; MOLLA AKHTERUZZAMAN; PAUL ANIKO V
权利人:UNIV NEW YORK STATE RES FOUND
摘要:A process for the de novo synthesis of a picornavirus using a cell-free medium has been developed. The cell-free medium is prepared from a lysate of mammalian cells from which the nuclei and mitochondria has been removed, endogenous mRNA deactivated and supplemented with nucleoside triphosphates, tRNA, amino acids and precursors necessary for generating proteins. The genomic RNA of the picornavirus or rhinovirus is added to the cell-free medium and after incubation at about 30° C. to 40° C. of from about 4 to 24 hours infectious, mature virus particles are formed.

专利号:US-6642367-B2
优先权日:1995-03-06
标 题:Process for the synthesis of 2′-O-substituted pyrimidines and oligomeric compounds therefrom
发明人：COOK PHILLIP DAN; SANGHVI YOGESH S; SPRANKLE KELLY G; ROSS BRUCE S; GRIFFEY RICH H; SPRINGER ROBERT H
权利人:ISIS PHARMACEUTICALS INC
摘要:Oligonucleotide analogs are disclosed having pyrimidine monomeric sub-units therein that are modified at the 2′ and 5′ positions. Monomeric sub-units having these modifications may be further modified at the 2′ position. Improved processes for the synthesis of 2′-O-substituted pyrimidine nucleosides are also provided. The processes feature alkylation of a 2,2′-anhydropyrimidine nucleoside or a 2S,2′-anhydropyrimidine nucleoside with a weak nucleophile in the presence of a Lewis acid.