专利号:US-12071646-B2 优先权日:2014-09-11 标 题 :Cells comprising mogroside pathway enzymes and uses thereof 发明人:ITKIN MAXIM; DAVIDOVICH-RIKANATI RACHEL; COHEN SHAHAR; PORTNOY VITALY; DORON-FAIGENBOIM ADI; PETREIKOV MARINA; SHEN SHMUEL; TADMOR YAAKOV; BURGER YOSEF; LEWINSOHN EFRAIM; KATZIR NURIT; SCHAFFER ARTHUR A; OREN ELAD 权利人:THE STATE OF ISRAEL MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT AGRICULTURAL RES ORGANIZATION ARO VO 摘要:Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.
专利号:US-12285423-B2 优先权日:2017-06-28 标题 :Composition for enhancing mitochondrial function 发明人:ANDREEVA LARISA; POMYTKIN IGOR ANATOLIEVICH; SKLADTCHIKOVA GALINA NONINA 权利人:MITOCHOLINE LTD 摘要:A method and composition for enhancing synthesis of adenosine triphosphate (ATP) in mitochondria of brain cells of a human includes providing the composition including a synergistic compound of choline cation and succinate anion (2-) in a molar ratio of choline:succinate of 2:1 in the form of dicholine succinate (DiSu) in a daily dosage that synergistically increases ATP levels in brain cells. The method and composition are formulated to affect frequency and/or severity of various conditions by regulating ATP levels in brain cells. The method and composition are formulated to reduce rate of decline in the brain under conditions of cerebral hypoxia, e.g., due to aging, injury, illness, and the similar conditions.
专利号:US-2018237819-A1 优先权日:2011-11-23 标 题 :Methods and Materials for Enzymatic Synthesis of Mogroside Compounds
专利号:WO-2025209017-A1 优先权日:2024-04-02 标题 :Engineered yeast capable of producing mogrol, siamenoside i, mogroside iiix, mogroside iva, and/or mogroside v and use thereof 发明人:HUA JUN; LI WEI; PEI LIANG; LI ZHENGHONG; PAN YUE 权利人:SICHUAN INGIA BIOSYNTHETIC CO LTD 摘要:The present invention relates to an engineered yeast capable of producing mogrol, siamenoside I, mogroside IIIX, mogroside IVA, and/or mogroside V and the use thereof. The present invention provides an engineered yeast capable of producing mogrol, siamenoside I, mogroside IIIX, mogroside IVA, and/or mogroside V. By optimizing and modifying chassis cells, a metabolic pathway of microorganisms is changed, or an expression level of related genes is regulated, thereby enhancing the synthesis efficiency and yield of target products, while improving the production efficiency.
专利号:CA-2853677-C 优先权日:2011-11-23 标 题:Methods and materials for enzymatic synthesis of mogroside compounds
专利号:US-2014308698-A1 优先权日:2011-11-23 标题:Methods and Materials for Enzymatic Synthesis of Mogroside Compounds
摘要:Yan H, Tao L, Qu X, Zhou L, Zhang S. Quantitative determination of mogroside V in rat plasma by LC-MS/MS and its application to a pharmacokinetic study. Pak J Pharm Sci. 2018 May;31(3):867–73.