CAS: 84325-23-5; 7-(((2S,3R,4R,5S,6R)-3,4-Dihydroxy-6-(Hydroxymethyl)-5-(((2S,3R,4S,5R,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Oxy)Tetrahydro-2H-Pyran-2-yl)Oxy)-4-Methyl-2H-Chromen-2-One

该化合物是广泛用于酶实验的含氟基质,特别是用于检测β-伽勒actosidase 活动,其主要优势在于其高度敏感,因为酶水解释放荧光4-甲基苯丙酮(4-MU),即使在低酶浓度的情况下也能够进行精确的量化;该化合物因其稳定性,水溶性,与各种生物化学和微生物应用的兼容性,包括报告者基因测定和微生物识别,而具有价值;其用于β-伽勒活性的特点尽量减少了交叉活动,确保了研究和诊断环境中的可靠结果;该产品因其稳健的性能和可复制的结果,通常用于微生物学,分子生物学和临床诊断.

英文名称7-(((2S,3R,4R,5S,6R)-3,4-Dihydroxy-6-(Hydroxymethyl)-5-(((2S,3R,4S,5R,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Oxy)Tetrahydro-2H-Pyran-2-yl)Oxy)-4-Methyl-2H-Chromen-2-One
中文名称4-甲基伞形-β-D-乳吡喃糖苷
IUPAC名称7-(((2S,3R,4R,5S,6R)-3,4-dihydroxy-6-(hydroxymethyl)-5-(((2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)oxy)-4-methyl-2H-chromen-2-one
其它别名7-[(2S,3R,4R,5S,6R)-3,4-Dihydroxy-6-(Hydroxymethyl)-5-[(2S,3R,4S,5R,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Oxan-2-Yl]Oxyoxan-2-Yl]Oxy-4-Methylchromen-2-One; 4-Methylumbelliferyl Beta-D-Lactoside; 4-Methylumbelliferyl-Beta-D-Lactoside; Sch
CAS编号84325-23-5
MFCD编号:MFCD00083406
分子式:C22H28O13分子量:500.45
产品CID: 1831768
产品分类生物化工 → 糖类化合物 → 双糖

相似结构搜索
InChIKey: PRTGXBPFDYMIJH-KSFLKEQHSA-N
PRTGXBPFDYMIJH-LTRTYOHLNA-N
计算化学: 氢键受体数13.0氢键供体数7.0可旋转键数6.0

物理性质

熔点>178°C (分解)
沸点830.319 °C at 760 mmHg
闪点286.047 °C
密度1.658g/cm3
PSA:208.74
LogP:-2.8957
折射率1.677
蒸汽压0 mmHg at 25 °C
溶解性DMF: Soluble
敏感性按规定使用和贮存的不会分解
外观形态白色至类白色固体
储存条件-20°C
产品应用该化合物用于乙酰丙烯二酰乙醚(CE7A),异丁烯二烯酶I(CEL7B)和苯丙二烯二甲酰丙胺酯(Β-GLUCOSIDASE)的底质.

上下游产品

CAS号90-33-5 4-甲基伞形酮 | CAS号92052-38-5 α-D-Glucopyrano...

合成工艺路线路线简述

合成目标产物 4-Methylumbelliferyl-Beta-D-Lactoside 主要起始原料 2H-1-Benzopyran-2-One, 4-Methyl-7-[[2,3,6-Tri-O-Acetyl-4-O-(2,3,4,6-Tetra-O-Acetyl-β-D-Galactopyranosyl)-β-D-Glucopyranosyl]Oxy]-
178450-89-0 = 84325-23-5
反应条件:1.1 Reagents: Sodium Methoxide Solvents: Methanol
标题:A Fluorometric Assay Of Ceramide Glycanase With 4-Methylumbelliferyl β-D-Lactoside Derivatives
作者:Wang,Lai-Xi; Et Al
参考文献:Glycoconjugate Journal 日期:1996 卷标:13(3) 页码:359-365]

5980-33-6 + 4753-07-5 = 84325-23-5
反应条件:1.1 Reagents: Tetrabutylammonium Hydrogen Sulfate Solvents: Chloroform,Water2.1 Reagents: Sodium Methoxide Solvents: Methanol
标题:A Fluorometric Assay Of Ceramide Glycanase With 4-Methylumbelliferyl β-D-Lactoside Derivatives
作者:Wang,Lai-Xi; Et Al
参考文献:Glycoconjugate Journal 日期:1996 卷标:13(3) 页码:359-365]

178450-89-0 = 84325-23-5
反应条件:1.1 Reagents: Sodium Methoxide Solvents: Methanol; 1 H,Rt
标题:Synthesis And Fluorescence Properties Of Coumarin Glycosides And Triazoylglycosides
作者:Wu,Zheng; Et Al
参考文献:Chemical Research In Chinese Universities 日期:2013 卷标:29(3) 页码:460-465]

178450-89-0 = 84325-23-5
反应条件:1.1 Reagents: Sodium Methoxide Solvents: Methanol; 10 H,Rt
标题:A Multifunctional Pasteurella Multocida Sialyltransferase: A Powerful Tool For The Synthesis Of Sialoside Libraries
作者:Yu,Hai; Et Al
参考文献:Journal Of The American Chemical Society 日期:2005 卷标:127(50) 页码:17618-17619]

90-33-5 + 106256-81-9 = 84325-23-5
反应条件:1.1 Solvents: Dichloromethane; 30 Min,Rt1.2 Reagents: Boron Trifluoride Etherate; -10 °C; -10 °C -> 0 °C; Overnight,0 °C2.1 Reagents: Sodium Methoxide Solvents: Methanol; 10 H,Rt
标题:A Multifunctional Pasteurella Multocida Sialyltransferase: A Powerful Tool For The Synthesis Of Sialoside Libraries
作者:Yu,Hai; Et Al
参考文献:Journal Of The American Chemical Society 日期:2005 卷标:127(50) 页码:17618-17619]

90-33-5 + 4753-07-5 = 84325-23-5
反应条件:1.1 Reagents: Sodium Carbonate Catalysts: Tetrabutylammonium Bromide Solvents: Chloroform,Water; Rt1.2 Solvents: Chloroform; 50 - 55 °C; 16 H,50 - 55 °C2.1 Reagents: Sodium Methoxide Solvents: Methanol; 1 H,Rt
标题:Synthesis And Fluorescence Properties Of Coumarin Glycosides And Triazoylglycosides
作者:Wu,Zheng; Et Al
参考文献:Chemical Research In Chinese Universities 日期:2013 卷标:29(3) 页码:460-465]

141-97-9 = 84325-23-5
反应条件:1.1 Reagents: Sulfuric Acid Solvents: Water; 24 H,Rt2.1 Reagents: Sodium Carbonate Catalysts: Tetrabutylammonium Bromide Solvents: Chloroform,Water; Rt2.2 Solvents: Chloroform; 50 - 55 °C; 16 H,50 - 55 °C3.1 Reagents: Sodium Methoxide Solvents: Methanol; 1 H,Rt
标题:Synthesis And Fluorescence Properties Of Coumarin Glycosides And Triazoylglycosides
作者:Wu,Zheng; Et Al
参考文献:Chemical Research In Chinese Universities 日期:2013 卷标:29(3) 页码:460-465

📜羟甲香豆素置于4 A Molecular Sieve,三氟化硼乙醚,Sodium Methylate体系中,用甲醇,二氯甲烷作为反应溶剂,化学反应 10.0H,反应生成 4-甲基伞形-β-D-乳吡喃糖苷
参考文献:A Multifunctional Pasteurella Multocida Sialyltransferase: A Powerful Tool For The Synthesis Of Sialoside Libraries
标题:A Multifunctional Pasteurella Multocida Sialyltransferase: A Powerful Tool For The Synthesis Of Sialoside Libraries
摘要:A Multifunctional Sialyltransferase Has Been Cloned From Pasteurella Multocida Strain P-1059 And Expressed In E. Coli As A Truncated C-Terminal His6-Tagged Recombinant Protein (Tpm0188Ph). Biochemical Studies Indicate That The Obtained Protein Is (1) An Alpha2,3-Sialyltransferase (Main Function),(2) An Alpha2,6-Sialyltransferase,(3) An Alpha2,3-Sialidase,And (4) An Alpha2,3-Trans-Sialidase. The Recombinant Tpm0188Ph Is A Powerful Tool In The Synthesis Of Structurally Diverse Sialoside Libraries Due To Its Relaxed Substrate Specificity,High Solubility,High Expression Level,And Multifunctionality.
DOI:10.1021/ja0561690