专利号:US-8809023-B2 优先权日:2009-08-19 标题 :Variants of glucoamylase 发明人:DEGN PETER EDVARD; BOTT RICHARD; VROEMEN CASPER WILLEM; SCHEFFERS MARTIJN SILVAN; AEHLE WOLFGANG 权利人:DEGN PETER EDVARD; BOTT RICHARD; VROEMEN CASPER WILLEM; SCHEFFERS MARTIJN SILVAN; AEHLE WOLFGANG; DANISCO US INC 摘要:The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
专利号:US-6534637-B2 优先权日:2001-02-12 标 题 :Synthesis of chlorophenol red glucuronic acid 发明人:SHEN GENE G Y; OH CHAN S; YANCEY STEPHANIE D; ADAMS CRAIG W 权利人:BECKMAN COULTER INC 摘要:This invention provides beta-D-Glucuronidase substrates of the formula:whereinR1, R2, and R7-R12 are independently selected from the group consisting of:hydrogen, fluorine, chlorine, bromine, iodine, alkyl, hydroxyl, alkoxy, carboxyl and nitro groups;R3-R6 are independently selected from the group consisting of hydrogen, fluorine, chlorine, bromine, iodine, nitro and amino; andM+ is selected from the group consisting of: proton, lithium, sodium, potassium, magnesium, calcium, barium, and ammonium ion.
专利号:US-2006131160-A1 优先权日:2002-11-26 标题:Method of preparing dendrons and dendrimers with heterocyclic substructures of imino-ethers and derivatives using non-standard means and microwaves 发明人:MARRERO TERRERO ALMA L; CONTRERAS ALARCON ORESTES R 权利人:CNIC CT NAC INVESTIGACIONES 摘要:Dendrons and dendrimers are prepared by synthetic procedures under microwaves starting from heterocyclic substructures of C2 and C4 substituted iminoethers or from their boronic, sulfonic and phosphoric derivatives, or from their N-substituted derivatives, cyclic and acyclics, their products of hydrolysis or the transformations of them in other pentagonal nitrogenated heterocycles with incorporation in some cases, of structural units of chromogenic or fluorogenic glucuronides in their cavities or in the dendrimeric surface, in dependence of the functionality of the outlying groups. Starting from different reactions of the iminoetheres with carboxylic acids, anhydrides, esters, bases, aldehydes, alkyl halides, amines, isocyanates, aromatic thiols, by pyrolysis or hydrolysis, in sequences of selected reactions, alternated and iteratives, with the use of procedures and non classic methods of synthesis under microwaves at power between 30 and 300 Watt at 2450 MHz, to achieve the formation of basic substructures for the construction of dendrons, arms or ramifications of dendritic structures of different generations, from G0 to G4, by a convergent strategy, in presence or absence of solvents and/or polymeric or mineral solid supports.
专利号:EP-1368363-A2 优先权日:2001-02-12 标 题 :Synthesis of chlorophenol red glucoronic acid
专利号:CN-101337982-A 优先权日:2007-07-05 标 题 :Synthesis of p-nitrobenzene-beta-D- glucoside and use thereof
专利号:CN-108715875-B 优先权日:2018-05-30 标题:A method for enzymatic chemical synthesis of heparan sulfate oligosaccharide with clear structure
参考文献:10.1186/1476-511x-10-116 摘要:An HM, Park SY, Lee DK, Kim JR, Cha MK, Lee SW, Lim HT, Kim KJ, Ha NJ. Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats. Lipids in Health and Disease. 2011 Jul 12;10(1):116. doi: 10.1186/1476-511x-10-116.