专利号:US-2023235372-A1 优先权日:2020-06-12 标题:Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes 发明人:RANDRIANJATOVO-GBALOU IRINA; SAID AHMED; RAHIER RENAUD 权利人:SYNHELIX 摘要:The invention relates to the field of nucleic acid synthesis or sequencing, more specifically to methods for ab-initio synthesis of nucleic acids, comprising contacting a nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3′-hydroxyl group of the nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of both ab-initio single-stranded nucleic acid synthesis activity and template-independent terminal nucleotidyl transferase activity.
专利号:US-2023235373-A1 优先权日:2020-06-12 标 题:Controlled template-independent synthesis of nucleic acids using thermostable enzymes 发明人:RANDRIANJATOVO-GBALOU IRINA; SAID AHMED; RAHIER RENAUD 权利人:SYNHELIX 摘要:The invention relates to methods for template-independent synthesis of nucleic acids, comprising iteratively contacting an initiator sequence comprising a 3′-end nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3-hydroxyl group of the 3-end nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of template-independent terminal nucleotidyl transferase activity but are devoid of a template-independent primase activity.
专利号:US-5043484-A 优先权日:1990-02-09 标 题 :Synthesis of hydroxyl-terminated polybutadienes using glycol ether solvents 发明人:KNIFTON JOHN F; MARQUIS EDWARD T 权利人:TEXACO CHEMICAL 摘要:Disclosed is an improvement in a process for polymerizing 1,3-butadiene selectively to form relatively low molecular weight hydroxyl-terminated polybutadiene oligomers in the presence of an aqueous solution of hydrogen peroxide which comprises the use of a glycol ether solvent to produce butadiene oligomers having a high degree of OH functionality and suppression of formation of solid or gel-type insoluble rubber by-products.
专利号:US-5349110-A 优先权日:1990-08-20 标题 :Synthesis of low molecular weight glycol ethers from oxiranes plus olefins 发明人:KNIFTON JOHN F 权利人:TEXACO CHEMICAL 摘要:Disclosed is a process for preparation of monoalkyl ethers by reacting low molecular weight olefins and the corresponding oxiranes, in the presence of a catalyst comprising an acidic heterogeneous or homogeneous catalyst, generally represented by the equation: ##STR1## where R, R', R' may be hydrogen or an alkyl radical.
专利号:WO-2009083739-A1 优先权日:2008-01-01 标 题:Method of synthesis of bosentan, its polymorphic forms and its salts
专利号:US-2022403435-A1 优先权日:2019-08-01 标 题 :Increasing Long-Sequence Yields In Template-Free Enzymatic Synthesis of Polynucleotides