CAS: 1991-87-3; (S)-2-Amino-3-(P-Tolyl)Propanoic Acid

上下游产品

CAS号93634-54-9 2-甲基-(4z)-(41-甲... | CAS号104-87-0 对甲基苯甲醛 | CAS号4599-47-7 DL-4-甲基苯丙氨酸 | CAS号104-81-4 4-甲基溴苄 | CAS号6955-13-1 2-acetamido-3-(... | CAS号74046-14-3 (S)-2-acetamido... | CAS号683809-83-8 4-fluoromethyl-... | CAS号479064-87-4 R-对甲基-Β-苯丙氨酸 | CAS号1866-39-3 对甲基肉桂酸 | CAS号4599-47-7 DL-4-甲基苯丙氨酸

4-Methylcinnamic Acid置于phenylalanine Ammonia Lyase From Lettuce (Lactuca Sativa L.) Lspal3,Ammonium Carbamate体系中,用 甲醇,水 用作溶剂,以99%的收率获得
参考文献:生菜 (Lactuca Sativa L.) 苯丙氨酸氨裂解酶作为生产 D-和 L-氨基酸的稳健生物催化剂的表征
标题:生菜 (Lactuca Sativa L.) 苯丙氨酸氨裂解酶作为生产 D-和 L-氨基酸的稳健生物催化剂的表征
摘要:苯丙氨酸解氨酶 (Pal) 催化l-苯丙氨酸可逆转化为相应的反式肉桂酸,从而提供了一条获得光学纯 α-氨基酸的途径.我们探索了生菜( Lactuca Sativa L.)基因组中编码的所有五种 Pal 的催化功能,此前已知这些 Pal 与伤口褐变有关.所有ls Pal 在氨消除反应中均对l-苯丙氨酸具有活性,并在 55-60 oc 和 Ph 9.0-9.5 时表现出最大活性.然而,其中的四种( Ls Pal1-Ls Pal4)表现出比ls Pal5 显着更高的活性和热稳定性,以及更广泛的底物谱,包括一些具有空间要求或给电子取代基的具有挑战性的底物.对最好的ls Pal3 进行了一组 21 种外消旋苯丙氨酸衍生物的动力学拆分,以及 21 种肉桂酸衍生物的氨加成.它在大多数情况下表现出优异的对映选择性,并且对于许多具有挑战性的非天然底物,其活性明显优于之前描述的 Pal,这证明了其在生物催化方面的巨大潜力.
Doi:10.1021/acs.Jafc.2C07890

专利信息

专利号:US-9938509-B2
优先权日:2010-12-08
标 题 :Biocatalysts and methods for the synthesis of armodafinil
发明人：ANG EE LUI; ALVIZO OSCAR; BEHROUZIAN BEHNAZ; CLAY MICHAEL D; COLLIER STEVEN J; EBERHARD ELLEN D; FAN FU; SONG SHIWEI; SMITH DEREK J; WIDEGREN MAGNUS; WILSON ROBERT; XU JUNYE; ZHU JUN
权利人:CODEXIS INC
摘要:The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (−)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.

专利号:US-2006211083-A1
优先权日:2005-01-21
标题:Products and processes for in vitro synthesis of biomolecules
发明人：KATZEN FEDERICO; KUDLICKI WIESLAW; FLETCHER JULIA
权利人:KATZEN FEDERICO; KUDLICKI WIESLAW; FLETCHER JULIA
摘要:Provided herein are products and processes for efficiently synthesizing biomolecules in vitro using cell-free extracts derived from mammalian cells and insect cells. In an embodiment, provided is a process for preparing a cell-free extract from insect cells and mammalian cells that efficiently synthesizes post-translationally modified target proteins (e.g., glycosylated target proteins). In some embodiments, a ribonucleic acid is synthesized in vitro that comprises a cap, a 5′ untranslated region comprising an 18S rRNA binding ribonucleotide sequence, and a target ribonucleotide sequence. It has been determined that such ribonucleic acids result in efficient in vitro synthesis of a target protein using cell-free extracts derived from non-rabbit mammalian cells and insect cells.

专利号:US-2024218014-A1
优先权日:2022-11-21
标 题:Synthesis of a cyclic peptide
发明人：BAUR PIUS BRUNO; CLEATOR EDWARD; DENIAU GILDAS; EISELE FRANK; FLÖGEL OLIVER; HUSTE ANJA; KEHL MARCEL ROMAN; LÖWENECK MARKUS; SILVA ROLANDO RAVELO; VORBERG RAFFAEL
权利人:JANSSEN PHARMACEUTICA NV
摘要:Processes for performing peptide synthesis, particularly for cyclic peptide synthesis are generally described. Reaction intermediates of the peptide synthesis are also described.

专利号:US-2023212788-A1
优先权日:2020-03-26
标 题:New method for automated on-demand biomolecular array synthesis
发明人：ZHANG YIXIN; LIN WEILIN
权利人:UNIV DRESDEN TECH
摘要:The invention provides an amphiphilic coating for the direct and rapid synthesis of an array of peptides and small molecular compounds on a planar surface of a solid support, comprising a hydrophilic chemical structure and a lipophilic group, wherein said peptides and small molecular compounds differ from spot to spot from each other in the chemical structure, characterized in that said amphiphilic coating possesses low wettability to polar aprotic solvents used in the array synthesis; said amphiphilic coating possessing low wettability is designed that it can be converted to a coating possessing high wettability by hydrolysis of the lipophilic group; and said amphiphilic coating comprises an amino group for the reaction with an electrophilic reagent. The invention further provides a solid support comprising said amphiphilic coating and a method for method for the direct and rapid synthesis of an array of peptides and small molecular compounds on a planar surface of a solid support, wherein said planar surface of a solid support comprises said amphiphilic coating. Said method includes the enhancing of the wettability of a glass surface to organic solvents to realize automated on-demand biomolecular array synthesis comprising both, peptides and small molecular compounds. The amphiphilic surface can be switched to a hydrophilic surface, resulting in high density arrays suitable for protein- and cell-based screening.

专利号:US-2022098155-A1
优先权日:2018-11-14
标 题 :Novel pathway for the synthesis of diazirines, that may or may not be enriched in nitrogen-15
发明人：REBOUL VINCENT; FRANCK XAVIER; GLACHET THOMAS; MARZAG HAMID
权利人:UNIV ROUEN NORMANDIE; UNIV CAEN; INSTITUT NAT DES SCIENCES APPLIQUEES DE ROUEN INSA; CENTRE NAT RECH SCIENT; ECOLE NAT SUPERIEURE DINGENIEURS DE CAEN
摘要:The present invention concerns a novel method for synthesising diazirines, that may or may not be enriched in nitro-gen-15, from amino acids or imines, via a one-pot synthesis method, comprising the reaction of the starting amino acid or imine with ammonia, which may or may not be enriched in nitrogen-15, and a hypervalent iodine oxidant. The present invention also relates to a method for synthesising ammonia enriched in nitrogen-15. The invention also concerns certain diazirines of formula (I) likely to be obtained by the claimed synthesis method, and also refers to the 15 N 2 -diazirines of formula (I′). The claimed diazirines can be used in photoaffinity labelling. The 15 N 2 -diazirines can also be used in hyperpolarisation, in particular in the medical imaging field.

专利号:US-9663771-B2
优先权日:2013-01-18
标题 :Engineered biocatalysts useful for carbapenem synthesis
发明人：SUKUMARAN JOLY; SMITH DEREK; YANG HONG; YEO WAN LIN; MOORE JEFFREY C
权利人:CODEXIS INC
摘要:The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem.