专利号:US-4617384-A 优先权日:1980-03-27 标 题 :Adaptor molecules for DNA and their application to synthesis of gene-derived products 发明人:NARANG SARAN A; WU RAY J 权利人:CORNELL RES FOUNDATION INC 摘要:Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endonucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.
专利号:US-8067204-B2 优先权日:2005-12-15 标 题 :Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin 发明人:SUGIURA NOBUO; SHIMOKATA SATOSHI; KIMATA KOJI 权利人:SUGIURA NOBUO; SHIMOKATA SATOSHI; KIMATA KOJI; SEIKAGAKU KOGYO CO LTD 摘要:A method for producing a chondroitin sugar chain comprises reacting a glucuronic acid donor, an N-acetyl galactosamine donor, a sugar receptor and a bacterial cell enzyme which synthesizes chondroitin in the presence of a surfactant. The surfactant is selected from polyoxyethylene octadecyl amine, n-decanoyl-N-methylglucamide, sodium cholate, n-octyl-β-D-thioglucopyranoside, n-nonyl-β-D-thiomaltopyranoside, sucrose monocholate, sucrose monocaprate, and sucrose monolaurate. The chondroitin sugar chain has all the following properties: a weight average molecular weight: 50,000 or more when measured by gel filtration chromatography; it is completely degraded to disaccharides with chondroitinase ABC; and when the sugar chain is decomposed with chondroitinase ABC and the decomposed products are subjected to a disaccharide analysis, substantially all of them correspond to an unsaturated disaccharide unit of chondroitin.
专利号:US-2006166315-A1 优先权日:2002-12-26 标题:Process for producing pnpase 发明人:MURAI MASATOSHI 权利人:MURAI MASATOSHI 摘要:The invention provides a process for producing PNPase, wherein PNPase can be produced easily with high efficiency, and problematic contamination with endotoxin in synthesis of a nucleic acid polymer as a raw material of pharmaceutical preparations can be reduced. PNPase is produced by Escherichia coil or the like having a T7 RNA polymerase gene, transformed with an expression vector having a PNPase gene and a T7 promoter ligated therein. For further facilitating the step of purifying PNPase, an expression vector having a tag gene is utilized and the culture time is prolonged.
专利号:US-2003215930-A1 优先权日:2000-12-08 标 题 :Genes involved in cyclododecanone degradation pathway 发明人:CHEN MARIO W; CHENG QIONG; GIBSON KATHARINE J; KOSTICHKA KRISTY N; THOMAS STUART M; NAGARAJAN VASANTHA 摘要:A 10 kb gene cluster has been isolated from Rhodococcus ruber SC1 comprising genes encoding enzymes useful for the synthesis of dodecanoic diacid from cyclododecanone and other cyclic intermediates. The six specific open reading frames have been identified that are associated with dodecanoic diacid biosynthesis. In addition to the expected substrates the enzymes of the instant invention have moderate specificity for C11-C15 compounds.
专利号:PL-246942-B1 优先权日:2021-12-02 标题 :Method of enzymatic synthesis of glochidon
专利号:PL-439727-A1 优先权日:2021-12-02 标 题:Method of enzymatic synthesis of glochidon
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合成参考文献
参考文献:10.1007/s00204-021-03204-y 摘要:Dionísio F, Araújo AM, Duarte-Araújo M, Bastos ML, Guedes de Pinho P, Carvalho F, Costa VM. Cardiotoxicity of cyclophosphamide's metabolites: an in vitro metabolomics approach in AC16 human cardiomyocytes. Arch Toxicol. 2022 Feb;96(2):653–71. doi: 10.1007/s00204-021-03204-y.