专利号:US-2023235372-A1 优先权日:2020-06-12 标题:Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes 发明人:RANDRIANJATOVO-GBALOU IRINA; SAID AHMED; RAHIER RENAUD 权利人:SYNHELIX 摘要:The invention relates to the field of nucleic acid synthesis or sequencing, more specifically to methods for ab-initio synthesis of nucleic acids, comprising contacting a nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3′-hydroxyl group of the nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of both ab-initio single-stranded nucleic acid synthesis activity and template-independent terminal nucleotidyl transferase activity.
专利号:WO-2013057134-A1 优先权日:2011-10-19 标 题:Synthesis of semiconductor nanoparticles using metal precursors comprising non- or weakly coordinating anions 发明人:BAHNMUELLER STEFAN; TIAN SUSAN; TIAN SHIZHE; HOHEISEL WERNER 权利人:BAYER IP GMBH; BAYER SOUTH EAST ASIA PTE LTD 摘要:The present invention relates to a method for the synthesis of semiconductor nanoparticles comprising elements of the groups IB, IIB, IIIA, IVA, VA or VIA of the periodic classification wherein the composition of the core nanoparticle is selected from the group consisting of IB-VIA, IIB-VIA, IIIA-VIA, IB-IIIA-VIA or IB-IIB-TVA-VIA, IIB-IIIA-VIA, IIB-TVA-VA or mixtures thereof, and at least one shell comprises elements of the groups IIB and VIA,, wherein in case the core composition is IIB-VIA the shell comprises at least a further element selected from the groups IB, IIIA, IVA or VA, using metal precursors comprising non- or weakly coordinating anions. It further relates to the consecutive process for the synthesis that includes first the preparation of a binary nanoparticle system then modified to make a ternary system which again can be further modified to make a quaternary system.
专利号:US-2023235373-A1 优先权日:2020-06-12 标 题:Controlled template-independent synthesis of nucleic acids using thermostable enzymes 发明人:RANDRIANJATOVO-GBALOU IRINA; SAID AHMED; RAHIER RENAUD 权利人:SYNHELIX 摘要:The invention relates to methods for template-independent synthesis of nucleic acids, comprising iteratively contacting an initiator sequence comprising a 3′-end nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3-hydroxyl group of the 3-end nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of template-independent terminal nucleotidyl transferase activity but are devoid of a template-independent primase activity.
专利号:US-11447454-B2 优先权日:2015-08-07 标题:Synthesis of benzodiazepine derivatives 发明人:BOYCE MALCOLM JAMES; THOMSEN LIV; GILBERT DONALD ALAN; WOOD DAVID 权利人:TRIO MEDICINES LTD 摘要:The invention relates to processes for the synthesis of benzodiazepine derivatives of Formula I:
专利号:US-8853132-B2 优先权日:2008-11-27 标题 :Directed synthesis of oligophosphoramidate stereoisomers 发明人:HEINDL DIETER; KESSLER DIRK; ROESLER ANGELIKA; SEIDEL CHRISTOPH; THUER WILMA 权利人:ROCHE DIAGNOSTICS OPERATIONS 摘要:The trivalent phosphorous atom of a compound is reacted with a reagent in such a manner that a stable phosphate mimetic or a specifier is formed. Phosphoramidites with a phosphorous atom containing at least one hydroxyl residue which is provided with a protective group are reacted for this purpose with a free hydroxyl group: In the first synthesis cycle the hydroxyl group is linked to a solid support via a cleavable or non-cleavable linker. In further synthesis cycles the hydroxyl group is created by cleavage of the protective group from the growing oligomer. This results in formation of a phosphorous acid triester which is reacted with azides. By selecting suitable monomers for the synthesis which have a defined stereoconformation compounds of Formula 1 are produced in a stereocontrolled manner.
专利号:US-7541165-B2 优先权日:2001-10-30 标 题 :Molecular detection systems utilizing reiterative oligonucleotide synthesis 发明人:HANNA MICHELLE M 权利人:RIBOMED BIOTECHNOLOGIES INC 摘要:The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.
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