CAS: 28542-78-1; Zeatin Riboside

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CAS号5451-40-1 2,6-二氯嘌呤 | CAS号13114-27-7 玉米素

📜4-[(2-Chloro-7H-Purin-6-yl)Amino]-2-Methylbut-2-En-1-Ol置于甲醇,二氯二茂钛,三氟甲磺酸三甲基硅酯,异丙基溴化镁,Sodium Methylate体系中,用 四氢呋喃,乙腈 用作溶剂,化学反应 48.0H,反应生成反式玉米素核糖甙
参考文献:Peroxide-Shunt Substrate-Specificity For The Salmonella Typhimurium O2-Dependent Trna Modifying Monooxygenase (Miae)
标题:Peroxide-Shunt Substrate-Specificity For The Salmonella Typhimurium O2-Dependent Trna Modifying Monooxygenase (Miae)
摘要:Post-Transcriptional Modifications Of Trna Are Made To Structurally Diversify Trna. These Modifications Alter Noncovalent Interactions Within The Ribosomal Machinery,Resulting In Phenotypic Changes Related To Cell Metabolism,Growth,And Virulence. Miae Is A Carboxylate Bridged,Nonheme Diiron Monooxygenase,Which Catalyzes The O-2-Dependent Hydroxylation Of A Hypermodified-Trna Nucleoside At Position 37 (2-Methylthio-N-6-Isopentenyl-Adenosine(37)-Trna) [designated Ms(2)I(6)A(37)]. In This Work,Recombinant Miae Was Cloned From Salmonella Typhimurium,Purified To Homogeneity,And Characterized By Uv-Visible And Dual-Mode X-Band Epr Spectroscopy For Comparison To Other Nonheme Diiron Enzymes. Additionally,Three Nucleoside Substrate-Surrogates (I(6)A,Cl(2)I(6)A,And Ms(2)I(6)A) And Their Corresponding Hydroxylated Products (Io(6)A,Cl(2)Io(6)A,And Ms(2)Io(6)A) Were Synthesized To Investigate The Chemo-And Stereospecificity Of This Enzyme. In The Absence Of The Native Electron Transport Chain,The Peroxide-Shunt Was Utilized To Monitor The Rate Of Substrate Hydroxylation. Remarkably,Regardless Of The Substrate (I(6)A,Cl(2)I(6)A,And Ms(2)I(6)A) Used In Peroxide-Shunt Assays,Hydroxylation Of The Terminal Isopentenyl-C4-Position Was Observed With >97% E-Stereoselectivity. No Other Nonspecific Hydroxylation Products Were Observed In Enzymatic Assays. Steady-State Kinetic Experiments Also Demonstrate That The Initial Rate Of Miae Hydroxylation Is Highly Influenced By The Substituent At The C2-Position Of The Nucleoside Base (V(0)/[e] For Ms(2)I(6)A > I(6)A > Cl(2)I(6)A). Indeed,The >3-Fold Rate Enhancement Exhibited By Miae For The Hydroxylation Of The Free Ms(2)I(6)A Nucleoside Relative To I(6)A Is Consistent With Previous Whole Cell Assays Reporting The Ms(2)Io(6)A And Io(6)A Product Distribution Within Native Trna-Substrates. This Observation Suggests That The Nucleoside C2-Substituent Is A Key Point Of Interaction Regulating Miae Substrate Specificity:
Doi:10.1021/bi4000832

专利信息

专利号:US-8759034-B2
优先权日:2009-12-22
标 题 :Thermostable biocatalyst combination for nucleoside synthesis
发明人：MONTILLA AREVALO RAFAEL; DERONCELÉTHOMAS VICTOR MANUEL; LÓPEZ GÓMEZ CRISTINA; PASCUAL GILABERT MARTA; ESTEVEZ COMPANY CARLOS; CASTELLS BOLIART JOSEP
权利人:PLASMIA BIOTECH S L
摘要:A recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterized in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNpase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.

专利号:US-2016271177-A1
优先权日:2015-03-18
标 题:Process for bio synthesis of nano arsenic trioxide and its use in treatment of diseases including cancer

专利号:RU-2569110-C2
优先权日:2009-12-22
标题:Combination of thermo stable biocatalysts for synthesis of nucleosides

专利号:EP-2897906-A1
优先权日:2012-09-20
标 题:Process for bio synthesis of nano arsenic trioxide and its use in treatment of diseases including cancers

专利号:EP-2897906-B1
优先权日:2012-09-20
标题 :Process for bio synthesis of nano arsenic trioxide and its use in treatment of diseases including cancers
发明人：BENDALE YOGESH NARAYAN; BENDALE VINEETA YOGESH
权利人:BENDALE YOGESH NARAYAN; BENDALE VINEETA YOGESH

专利号:WO-2014045083-A1
优先权日:2012-09-20
标题:Process for bio synthesis of nano arsenic trioxide and its use in treatment of diseases including cancers